Streptococcus satellite phage Javan429
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZT03|A0A4D5ZT03_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan429 OX=2558697 GN=JavanS429_0014 PE=4 SV=1
MM1 pKa = 7.61 NNTQVYY7 pKa = 8.59 QAQTTNHH14 pKa = 6.71 GIARR18 pKa = 11.84 ALGEE22 pKa = 4.87 EE23 pKa = 3.69 IRR25 pKa = 11.84 MITDD29 pKa = 3.35 VEE31 pKa = 4.1 EE32 pKa = 4.15 TNYY35 pKa = 10.62 NGHH38 pKa = 6.17 GEE40 pKa = 4.18 IIVEE44 pKa = 4.21 YY45 pKa = 9.21 TSDD48 pKa = 3.37 VALTKK53 pKa = 10.85 LFDD56 pKa = 5.08 RR57 pKa = 11.84 IKK59 pKa = 10.53 SLYY62 pKa = 9.62 EE63 pKa = 3.72 KK64 pKa = 10.85 GEE66 pKa = 4.17 SYY68 pKa = 7.74 EE69 pKa = 4.29 TKK71 pKa = 10.16 PFLHH75 pKa = 6.55 FKK77 pKa = 10.57 VLEE80 pKa = 4.74 DD81 pKa = 4.36 DD82 pKa = 4.8 VEE84 pKa = 4.48 PNPNGVFPAKK94 pKa = 9.85 EE95 pKa = 3.85 VADD98 pKa = 4.09 FLGVV102 pKa = 3.18
Molecular weight: 11.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.632
IPC2_protein 4.673
IPC_protein 4.533
Toseland 4.406
ProMoST 4.635
Dawson 4.482
Bjellqvist 4.622
Wikipedia 4.329
Rodwell 4.393
Grimsley 4.329
Solomon 4.469
Lehninger 4.431
Nozaki 4.596
DTASelect 4.698
Thurlkill 4.406
EMBOSS 4.355
Sillero 4.66
Patrickios 3.757
IPC_peptide 4.482
IPC2_peptide 4.647
IPC2.peptide.svr19 4.598
Protein with the highest isoelectric point:
>tr|A0A4D5ZNA9|A0A4D5ZNA9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan429 OX=2558697 GN=JavanS429_0006 PE=4 SV=1
MM1 pKa = 7.37 TNYY4 pKa = 10.27 DD5 pKa = 3.45 RR6 pKa = 11.84 AKK8 pKa = 10.18 RR9 pKa = 11.84 FYY11 pKa = 11.32 SRR13 pKa = 11.84 ATYY16 pKa = 10.67 ALSDD20 pKa = 3.97 FLHH23 pKa = 6.24 GRR25 pKa = 11.84 IDD27 pKa = 3.42 EE28 pKa = 4.27 QEE30 pKa = 4.16 FRR32 pKa = 11.84 KK33 pKa = 9.32 QWQRR37 pKa = 11.84 LADD40 pKa = 3.64 KK41 pKa = 10.62 AVNEE45 pKa = 4.21 MNKK48 pKa = 9.98 NN49 pKa = 3.35
Molecular weight: 6.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.111
IPC2_protein 9.063
IPC_protein 9.209
Toseland 9.633
ProMoST 9.414
Dawson 9.897
Bjellqvist 9.589
Wikipedia 10.101
Rodwell 10.131
Grimsley 9.999
Solomon 9.999
Lehninger 9.955
Nozaki 9.575
DTASelect 9.604
Thurlkill 9.721
EMBOSS 10.043
Sillero 9.809
Patrickios 7.629
IPC_peptide 9.984
IPC2_peptide 8.2
IPC2.peptide.svr19 8.03
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16
0
16
2699
48
484
168.7
19.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.669 ± 0.408
0.63 ± 0.076
6.002 ± 0.619
8.707 ± 0.792
4.224 ± 0.447
5.039 ± 0.361
1.704 ± 0.203
7.003 ± 0.555
9.856 ± 0.52
9.633 ± 0.9
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.149 ± 0.31
5.372 ± 0.349
2.594 ± 0.258
4.446 ± 0.496
4.891 ± 0.539
5.15 ± 0.444
5.521 ± 0.553
5.78 ± 0.409
0.741 ± 0.116
4.891 ± 0.226
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here