Ralstonia phage Cimandef

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Cimandefvirus; Ralstonia virus Cimandef

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G5B8S1|A0A7G5B8S1_9CAUD Peptidase S74 domain-containing protein OS=Ralstonia phage Cimandef OX=2759720 GN=B2_00060 PE=4 SV=1
MM1 pKa = 7.53LGIVQKK7 pKa = 9.44TATEE11 pKa = 3.89QLDD14 pKa = 3.56YY15 pKa = 11.26DD16 pKa = 3.64IDD18 pKa = 3.85FARR21 pKa = 11.84WMPDD25 pKa = 2.52GDD27 pKa = 4.01VLQGAGVAITPDD39 pKa = 4.47DD40 pKa = 3.74GTLASPAYY48 pKa = 9.25EE49 pKa = 3.65IDD51 pKa = 3.57GTVVKK56 pKa = 10.55VWLAGGTAGASYY68 pKa = 11.0NVDD71 pKa = 3.27VTVATAAGRR80 pKa = 11.84IKK82 pKa = 9.03EE83 pKa = 4.4TCFKK87 pKa = 10.58IRR89 pKa = 11.84VRR91 pKa = 11.84SCC93 pKa = 2.82

Molecular weight: 9.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G5B8N2|A0A7G5B8N2_9CAUD KilA-N domain-containing protein OS=Ralstonia phage Cimandef OX=2759720 GN=B2_00021 PE=4 SV=1
MM1 pKa = 7.27KK2 pKa = 10.28RR3 pKa = 11.84SAPLVRR9 pKa = 11.84KK10 pKa = 9.81APISRR15 pKa = 11.84GTSQLKK21 pKa = 7.54RR22 pKa = 11.84TAMKK26 pKa = 10.13KK27 pKa = 9.33RR28 pKa = 11.84APKK31 pKa = 9.76KK32 pKa = 10.5RR33 pKa = 11.84PGHH36 pKa = 6.05DD37 pKa = 2.84KK38 pKa = 11.21RR39 pKa = 11.84MLDD42 pKa = 3.15ACRR45 pKa = 11.84GQHH48 pKa = 6.34CYY50 pKa = 10.67LRR52 pKa = 11.84IPGVCRR58 pKa = 11.84DD59 pKa = 3.87DD60 pKa = 3.72TATTVPAHH68 pKa = 6.14RR69 pKa = 11.84NEE71 pKa = 4.18GKK73 pKa = 10.69GVGLKK78 pKa = 10.63VPDD81 pKa = 4.71KK82 pKa = 9.48YY83 pKa = 9.52TLPACYY89 pKa = 8.5WCHH92 pKa = 6.62YY93 pKa = 10.36EE94 pKa = 3.76YY95 pKa = 11.16DD96 pKa = 3.16QGNRR100 pKa = 11.84FTRR103 pKa = 11.84EE104 pKa = 3.58EE105 pKa = 3.83KK106 pKa = 10.48RR107 pKa = 11.84GLFNAAYY114 pKa = 8.38PRR116 pKa = 11.84WAAYY120 pKa = 9.92RR121 pKa = 11.84EE122 pKa = 4.09QRR124 pKa = 11.84LGLAAA129 pKa = 5.03

Molecular weight: 14.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

17322

31

4545

266.5

29.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.064 ± 0.511

0.762 ± 0.234

6.131 ± 0.251

6.42 ± 0.381

3.135 ± 0.129

8.642 ± 0.405

1.784 ± 0.141

4.422 ± 0.249

4.884 ± 0.314

6.847 ± 0.185

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.731 ± 0.137

3.677 ± 0.221

4.774 ± 0.215

4.295 ± 0.235

7.008 ± 0.306

5.554 ± 0.253

5.45 ± 0.237

6.443 ± 0.195

1.31 ± 0.155

2.667 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski