Acinetobacter phage Ab11510-phi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vieuvirus; unclassified Vieuvirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3T121|A0A7G3T121_9CAUD Uncharacterized protein OS=Acinetobacter phage Ab11510-phi OX=2735531 GN=phiAb1151011551_00013 PE=4 SV=1
MM1 pKa = 7.32EE2 pKa = 5.95WISVDD7 pKa = 3.02EE8 pKa = 4.76RR9 pKa = 11.84MPEE12 pKa = 3.95LDD14 pKa = 3.55VLVLIFNPFTHH25 pKa = 6.09EE26 pKa = 4.03TMHH29 pKa = 5.85TAKK32 pKa = 10.43LVEE35 pKa = 4.25IEE37 pKa = 4.28GEE39 pKa = 4.12EE40 pKa = 3.76WWFFEE45 pKa = 5.34SDD47 pKa = 4.85DD48 pKa = 5.51DD49 pKa = 5.42NLDD52 pKa = 3.57PQYY55 pKa = 11.14ASHH58 pKa = 6.84WMPLPEE64 pKa = 4.49PPKK67 pKa = 10.64NN68 pKa = 3.44

Molecular weight: 8.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3T7X8|A0A7G3T7X8_9CAUD Uncharacterized protein OS=Acinetobacter phage Ab11510-phi OX=2735531 GN=phiAb1151011551_00051 PE=4 SV=1
MM1 pKa = 7.55GLNFRR6 pKa = 11.84KK7 pKa = 9.94SIKK10 pKa = 9.1IAPGIRR16 pKa = 11.84VNVSKK21 pKa = 10.83KK22 pKa = 9.4GLSSVSVGGKK32 pKa = 7.46GARR35 pKa = 11.84VNVSKK40 pKa = 10.88KK41 pKa = 7.74GTRR44 pKa = 11.84TTVGIPGTGLSYY56 pKa = 11.42SKK58 pKa = 10.65FSSHH62 pKa = 5.44TKK64 pKa = 7.64KK65 pKa = 6.86TTRR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84EE71 pKa = 3.74PDD73 pKa = 3.28FNNPDD78 pKa = 3.84NVWGYY83 pKa = 9.69PKK85 pKa = 10.26SEE87 pKa = 4.14WIICGIILFIALMIFIWIISS107 pKa = 3.6

Molecular weight: 11.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

15382

62

1643

202.4

22.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.957 ± 0.623

0.897 ± 0.125

5.851 ± 0.159

7.444 ± 0.298

4.057 ± 0.211

6.183 ± 0.312

1.762 ± 0.195

6.189 ± 0.236

7.886 ± 0.349

8.815 ± 0.265

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.542 ± 0.177

5.643 ± 0.214

3.355 ± 0.294

4.486 ± 0.393

4.486 ± 0.181

6.144 ± 0.282

5.227 ± 0.291

6.215 ± 0.265

1.19 ± 0.123

3.673 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski