Kineosphaera limosa NBRC 100340

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Dermatophilaceae; Kineosphaera; Kineosphaera limosa

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4422 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K6XEK7|K6XEK7_9MICO Uncharacterized protein (Fragment) OS=Kineosphaera limosa NBRC 100340 OX=1184609 GN=KILIM_062_00010 PE=4 SV=1
MM1 pKa = 7.18LTSPDD6 pKa = 3.92LPSPPEE12 pKa = 4.12PTSHH16 pKa = 6.81CPAALALFEE25 pKa = 4.92EE26 pKa = 5.22LTTLCPDD33 pKa = 3.21TGDD36 pKa = 4.18ARR38 pKa = 11.84TQAALEE44 pKa = 4.12EE45 pKa = 4.6VLTLACDD52 pKa = 4.22RR53 pKa = 11.84VTAWII58 pKa = 4.0

Molecular weight: 6.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K6WTJ5|K6WTJ5_9MICO NAD(+) diphosphatase OS=Kineosphaera limosa NBRC 100340 OX=1184609 GN=KILIM_058_00280 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84THH17 pKa = 5.69GFRR20 pKa = 11.84LRR22 pKa = 11.84MSTRR26 pKa = 11.84AGRR29 pKa = 11.84AILAGRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 10.08GRR40 pKa = 11.84EE41 pKa = 3.61KK42 pKa = 10.93LSAA45 pKa = 3.78

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4422

0

4422

1442572

37

2845

326.2

34.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.846 ± 0.054

0.753 ± 0.011

6.09 ± 0.03

5.275 ± 0.034

2.729 ± 0.023

9.226 ± 0.034

2.211 ± 0.016

3.784 ± 0.024

1.771 ± 0.028

10.258 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.911 ± 0.015

1.784 ± 0.018

5.96 ± 0.031

3.241 ± 0.021

7.765 ± 0.039

5.256 ± 0.023

5.995 ± 0.025

8.754 ± 0.033

1.504 ± 0.016

1.887 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski