Shigella phage POCJ13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Sepvirinae; Diegovirus; Shigella virus POCJ13

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088CC19|A0A088CC19_9CAUD Uncharacterized protein OS=Shigella phage POCJ13 OX=1498227 PE=4 SV=1
MM1 pKa = 7.03EE2 pKa = 4.85QYY4 pKa = 10.38TGIILPHH11 pKa = 7.85LEE13 pKa = 4.01EE14 pKa = 5.1FRR16 pKa = 11.84QQQHH20 pKa = 5.56QPQQPEE26 pKa = 4.04ADD28 pKa = 3.34WVNPEE33 pKa = 3.8IPGPSVKK40 pKa = 9.48MCSHH44 pKa = 6.9TNVQSHH50 pKa = 5.76QPQQPEE56 pKa = 4.1ADD58 pKa = 3.26WVNPGDD64 pKa = 3.69TGTVCGMEE72 pKa = 4.02

Molecular weight: 8.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088CBN9|A0A088CBN9_9CAUD Uncharacterized protein OS=Shigella phage POCJ13 OX=1498227 PE=4 SV=1
MM1 pKa = 7.54AKK3 pKa = 10.02VFTPEE8 pKa = 3.43QRR10 pKa = 11.84EE11 pKa = 4.0EE12 pKa = 3.81LKK14 pKa = 11.05ARR16 pKa = 11.84IVEE19 pKa = 4.05LVRR22 pKa = 11.84LNGRR26 pKa = 11.84GTFRR30 pKa = 11.84QLATEE35 pKa = 4.18TGVSKK40 pKa = 9.4TAVRR44 pKa = 11.84RR45 pKa = 11.84LSRR48 pKa = 11.84EE49 pKa = 3.93LVVSGAIFDD58 pKa = 3.61SGYY61 pKa = 11.45GLFPSDD67 pKa = 4.0QARR70 pKa = 11.84KK71 pKa = 9.39DD72 pKa = 3.31WQNARR77 pKa = 11.84KK78 pKa = 9.55KK79 pKa = 10.6LSRR82 pKa = 11.84AKK84 pKa = 10.2AKK86 pKa = 10.32KK87 pKa = 9.68PVVIDD92 pKa = 3.65PGLIRR97 pKa = 11.84ALPDD101 pKa = 3.36GEE103 pKa = 3.56IRR105 pKa = 11.84RR106 pKa = 11.84YY107 pKa = 9.44DD108 pKa = 3.09RR109 pKa = 11.84RR110 pKa = 11.84YY111 pKa = 10.6NIICRR116 pKa = 11.84EE117 pKa = 4.11CRR119 pKa = 11.84NSEE122 pKa = 3.75TMQRR126 pKa = 11.84VLAFWRR132 pKa = 11.84GSAEE136 pKa = 3.63GLMFSPSS143 pKa = 2.94

Molecular weight: 16.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

18392

37

2793

232.8

25.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.341 ± 0.372

1.245 ± 0.212

5.97 ± 0.223

6.965 ± 0.302

3.257 ± 0.172

7.329 ± 0.523

1.762 ± 0.134

5.154 ± 0.366

5.85 ± 0.286

7.699 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.784 ± 0.14

4.491 ± 0.175

4.475 ± 0.314

4.513 ± 0.308

6.699 ± 0.325

6.166 ± 0.206

5.611 ± 0.206

6.367 ± 0.207

1.386 ± 0.122

2.936 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski