Christensenella minuta
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3119 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A136Q5C9|A0A136Q5C9_9FIRM RND transporter HAE1/HME family permease protein OS=Christensenella minuta OX=626937 GN=HMPREF3293_01478 PE=4 SV=1
MM1 pKa = 7.9 DD2 pKa = 3.76 RR3 pKa = 11.84 RR4 pKa = 11.84 KK5 pKa = 10.34 RR6 pKa = 11.84 FLTMLVCFVLAAFMLTACAGGGAPAATEE34 pKa = 4.23 SVAAGQGAEE43 pKa = 4.34 TEE45 pKa = 4.57 TPAATDD51 pKa = 3.23 TAEE54 pKa = 4.65 AGTEE58 pKa = 3.96 SSPAILTSADD68 pKa = 3.36 VGDD71 pKa = 3.76 VSGIEE76 pKa = 4.19 GWDD79 pKa = 3.74 GPVEE83 pKa = 4.34 SQVMTAGEE91 pKa = 4.8 DD92 pKa = 3.56 GDD94 pKa = 3.91 QFGTAEE100 pKa = 4.12 STVVVGKK107 pKa = 10.35 GPKK110 pKa = 10.35 GEE112 pKa = 4.56 VSTPWTEE119 pKa = 3.47 IALTDD124 pKa = 3.71 EE125 pKa = 4.65 EE126 pKa = 4.81 KK127 pKa = 11.01 EE128 pKa = 4.11 QIRR131 pKa = 11.84 SGNYY135 pKa = 7.99 KK136 pKa = 10.26 AAICFHH142 pKa = 6.16 YY143 pKa = 8.22 TTDD146 pKa = 2.97 DD147 pKa = 3.57 WYY149 pKa = 10.78 VMQRR153 pKa = 11.84 KK154 pKa = 9.31 GIEE157 pKa = 3.81 EE158 pKa = 4.15 TFADD162 pKa = 4.01 LGIEE166 pKa = 4.34 VVAVTDD172 pKa = 3.76 AGFSAEE178 pKa = 4.03 QQVSDD183 pKa = 3.99 IEE185 pKa = 4.57 NVLEE189 pKa = 4.45 LNPDD193 pKa = 4.07 IIISIPVDD201 pKa = 3.47 EE202 pKa = 4.94 TSTAVAYY209 pKa = 10.11 QKK211 pKa = 11.12 AADD214 pKa = 3.96 AGVKK218 pKa = 9.87 LVFMDD223 pKa = 3.98 NCPSNMTAGKK233 pKa = 10.13 DD234 pKa = 3.61 YY235 pKa = 11.23 VSIVAADD242 pKa = 4.1 RR243 pKa = 11.84 YY244 pKa = 9.0 GCGMICADD252 pKa = 4.73 LMARR256 pKa = 11.84 EE257 pKa = 4.37 LNYY260 pKa = 10.45 EE261 pKa = 3.96 GTVGVIYY268 pKa = 10.68 YY269 pKa = 10.32 DD270 pKa = 3.34 ADD272 pKa = 3.74 FFVTTRR278 pKa = 11.84 DD279 pKa = 3.17 CAGFVDD285 pKa = 4.96 RR286 pKa = 11.84 MKK288 pKa = 10.94 LKK290 pKa = 10.39 YY291 pKa = 9.95 PNIEE295 pKa = 3.83 IVTEE299 pKa = 3.96 MGFADD304 pKa = 3.52 ATLSGEE310 pKa = 4.34 VADD313 pKa = 5.9 AMLAQYY319 pKa = 10.49 PDD321 pKa = 3.07 IDD323 pKa = 4.86 GIFGSWDD330 pKa = 3.15 VPAEE334 pKa = 4.16 YY335 pKa = 9.88 IASSASAVGRR345 pKa = 11.84 DD346 pKa = 3.7 DD347 pKa = 5.82 LVVNCVGLGTTTASMTAGDD366 pKa = 4.8 GIINTISTQRR376 pKa = 11.84 PYY378 pKa = 11.4 DD379 pKa = 3.52 QGCTEE384 pKa = 5.1 ALLACYY390 pKa = 10.26 GLLGKK395 pKa = 9.7 DD396 pKa = 2.91 APEE399 pKa = 4.25 YY400 pKa = 9.32 VTVPALGVTKK410 pKa = 10.44 EE411 pKa = 4.02 GSEE414 pKa = 4.06 NSASLKK420 pKa = 10.38 DD421 pKa = 3.85 AYY423 pKa = 11.06 QLFYY427 pKa = 11.02 QIDD430 pKa = 4.05 TLPDD434 pKa = 3.0 WLQQLMM440 pKa = 4.09
Molecular weight: 46.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.884
IPC_protein 3.884
Toseland 3.681
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.783
Rodwell 3.719
Grimsley 3.579
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.19
Thurlkill 3.719
EMBOSS 3.795
Sillero 4.012
Patrickios 1.392
IPC_peptide 3.859
IPC2_peptide 3.986
IPC2.peptide.svr19 3.881
Protein with the highest isoelectric point:
>tr|A0A136Q814|A0A136Q814_9FIRM DEAD-box ATP-dependent RNA helicase CshA family protein OS=Christensenella minuta OX=626937 GN=HMPREF3293_00367 PE=3 SV=1
MM1 pKa = 7.31 KK2 pKa = 10.07 RR3 pKa = 11.84 KK4 pKa = 9.67 KK5 pKa = 10.65 SPWRR9 pKa = 11.84 MLHH12 pKa = 5.97 GRR14 pKa = 11.84 GISNVKK20 pKa = 9.99 HH21 pKa = 5.61 IRR23 pKa = 11.84 KK24 pKa = 9.58 APGTLSGAFPRR35 pKa = 11.84 RR36 pKa = 11.84 SRR38 pKa = 11.84 RR39 pKa = 11.84 GTGQRR44 pKa = 11.84 LLTVAFFF51 pKa = 4.35
Molecular weight: 5.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.508
IPC2_protein 11.155
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.618
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.34
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.137
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3119
0
3119
890553
29
4909
285.5
31.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.169 ± 0.053
1.466 ± 0.021
5.478 ± 0.035
7.202 ± 0.049
4.079 ± 0.036
8.03 ± 0.042
1.628 ± 0.02
6.821 ± 0.045
6.143 ± 0.042
8.803 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.101 ± 0.027
3.807 ± 0.026
3.75 ± 0.028
3.136 ± 0.023
5.069 ± 0.044
5.406 ± 0.037
5.347 ± 0.051
7.093 ± 0.039
0.838 ± 0.014
3.636 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here