Jatropha leaf crumple virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; unclassified Begomovirus

Average proteome isoelectric point is 7.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A089VMH8|A0A089VMH8_9GEMI C4 protein OS=Jatropha leaf crumple virus OX=1547577 GN=C4 PE=3 SV=1
MM1 pKa = 8.06WDD3 pKa = 3.35PLLNEE8 pKa = 4.99FPDD11 pKa = 3.91SVHH14 pKa = 6.7GLRR17 pKa = 11.84CMFVVIYY24 pKa = 9.61VQLVEE29 pKa = 4.28GTYY32 pKa = 10.46FPDD35 pKa = 3.59TLGHH39 pKa = 5.81EE40 pKa = 5.29LIRR43 pKa = 11.84DD44 pKa = 4.64LISVIRR50 pKa = 11.84AKK52 pKa = 10.86NYY54 pKa = 9.48VEE56 pKa = 3.75ATSRR60 pKa = 11.84YY61 pKa = 7.89HH62 pKa = 6.53HH63 pKa = 5.85FHH65 pKa = 6.33SRR67 pKa = 11.84LEE69 pKa = 4.21GSSPSEE75 pKa = 3.96LGQPIYY81 pKa = 10.35QPCICPHH88 pKa = 6.38CPRR91 pKa = 11.84HH92 pKa = 5.95KK93 pKa = 10.51KK94 pKa = 10.55AGLDD98 pKa = 3.4QQAQEE103 pKa = 4.26SQAQVVQDD111 pKa = 3.92LQKK114 pKa = 10.45PRR116 pKa = 11.84CPP118 pKa = 3.73

Molecular weight: 13.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A089XBE4|A0A089XBE4_9GEMI Replication enhancer OS=Jatropha leaf crumple virus OX=1547577 GN=C3 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.95IIISTPGSKK16 pKa = 9.65VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNLDD24 pKa = 3.01SPYY27 pKa = 10.21TSRR30 pKa = 11.84ASAPTVRR37 pKa = 11.84VTRR40 pKa = 11.84RR41 pKa = 11.84QAWTNRR47 pKa = 11.84PRR49 pKa = 11.84NRR51 pKa = 11.84KK52 pKa = 7.72PKK54 pKa = 8.34WYY56 pKa = 9.78RR57 pKa = 11.84IYY59 pKa = 10.66RR60 pKa = 11.84SPDD63 pKa = 3.09VPKK66 pKa = 10.81GCEE69 pKa = 4.39GPCKK73 pKa = 10.05VQSFEE78 pKa = 4.02SRR80 pKa = 11.84HH81 pKa = 5.54DD82 pKa = 3.51VVHH85 pKa = 6.75IGKK88 pKa = 9.41VMCISDD94 pKa = 3.6VTRR97 pKa = 11.84GNGFTHH103 pKa = 7.34RR104 pKa = 11.84EE105 pKa = 3.9GKK107 pKa = 9.86RR108 pKa = 11.84FCVKK112 pKa = 9.86SVYY115 pKa = 9.68MGGKK119 pKa = 8.88PPPDD123 pKa = 3.29EE124 pKa = 4.47NIKK127 pKa = 9.33TKK129 pKa = 10.67NHH131 pKa = 6.03TNTVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.13KK148 pKa = 10.28PQDD151 pKa = 3.56FGEE154 pKa = 4.33VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.63NMYY172 pKa = 9.53RR173 pKa = 11.84DD174 pKa = 3.71RR175 pKa = 11.84YY176 pKa = 8.85QVLRR180 pKa = 11.84KK181 pKa = 9.18WSSTVTGGQYY191 pKa = 10.8ASKK194 pKa = 9.43EE195 pKa = 3.85QAIVRR200 pKa = 11.84RR201 pKa = 11.84FFRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.1YY219 pKa = 8.23EE220 pKa = 4.02NHH222 pKa = 6.47TEE224 pKa = 3.99NALMLYY230 pKa = 7.54MACTPPFNPVYY241 pKa = 9.35ATLKK245 pKa = 8.77IRR247 pKa = 11.84IYY249 pKa = 10.88FFDD252 pKa = 3.66SVSNN256 pKa = 3.94

Molecular weight: 29.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1197

100

352

171.0

19.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.845 ± 0.667

2.506 ± 0.629

5.013 ± 0.496

4.177 ± 0.348

4.511 ± 0.397

5.848 ± 0.383

3.843 ± 0.591

5.263 ± 0.691

4.261 ± 0.978

6.516 ± 0.99

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.005 ± 0.407

5.096 ± 0.587

7.185 ± 0.653

5.013 ± 0.595

7.937 ± 1.06

8.605 ± 0.694

5.931 ± 0.796

6.266 ± 1.01

1.337 ± 0.19

3.843 ± 0.349
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski