Lausannevirus
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 444 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F2WL52|F2WL52_9VIRU Putative nucleoside deaminase OS=Lausannevirus OX=999883 GN=LAU_0124 PE=4 SV=1
MM1 pKa = 7.49 SRR3 pKa = 11.84 LQGFFKK9 pKa = 10.51 DD10 pKa = 3.47 IPIVGSYY17 pKa = 10.37 SALPPVLPRR26 pKa = 11.84 GSLVFLTSDD35 pKa = 3.11 EE36 pKa = 4.21 TLYY39 pKa = 11.38 VSDD42 pKa = 3.59 GTTWNATGGDD52 pKa = 3.95 IGPLAAQVAQNTADD66 pKa = 3.22 IGVLQTQVGTLQGEE80 pKa = 4.73 VATNTLDD87 pKa = 3.32 IGTLQTQVSTNTSDD101 pKa = 3.09 IGTLQTQVAGNSTNISALQTDD122 pKa = 4.12 VATLEE127 pKa = 4.4 GQVATNTTDD136 pKa = 2.67 IGTLQSQVATNTTNIQQNSDD156 pKa = 4.12 SITALLINQSQGFSILDD173 pKa = 3.48 NTQTRR178 pKa = 11.84 AYY180 pKa = 6.68 TQPISRR186 pKa = 11.84 VDD188 pKa = 3.54 VASVSPTVIYY198 pKa = 10.0 TLGIPLAININAVYY212 pKa = 10.13 LIQWNVLAHH221 pKa = 6.05 YY222 pKa = 7.5 EE223 pKa = 4.06 TANVYY228 pKa = 9.77 FAVGQSSVLSNGVGSLVSVFGTTTNQTNNSGAANIAVTADD268 pKa = 3.8 FSTPNFRR275 pKa = 11.84 LIVTSDD281 pKa = 3.24 SAVNTTYY288 pKa = 10.59 SGYY291 pKa = 11.11 VIVTNILNIVPP302 pKa = 4.08
Molecular weight: 31.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.808
IPC_protein 3.783
Toseland 3.554
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.783
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.745
Nozaki 3.923
DTASelect 4.215
Thurlkill 3.643
EMBOSS 3.783
Sillero 3.923
Patrickios 0.604
IPC_peptide 3.783
IPC2_peptide 3.884
IPC2.peptide.svr19 3.794
Protein with the highest isoelectric point:
>tr|F2WM03|F2WM03_9VIRU Uncharacterized protein OS=Lausannevirus OX=999883 GN=LAU_0426 PE=4 SV=1
MM1 pKa = 7.46 LRR3 pKa = 11.84 FSHH6 pKa = 6.3 SKK8 pKa = 9.71 YY9 pKa = 10.26 FIRR12 pKa = 11.84 KK13 pKa = 8.02 ILVRR17 pKa = 11.84 CLCVNRR23 pKa = 11.84 EE24 pKa = 3.95 CSPLQKK30 pKa = 10.5 LRR32 pKa = 11.84 TATIGTRR39 pKa = 11.84 PQHH42 pKa = 6.33 QDD44 pKa = 3.23 TPCWASFLMGGTTVPSFCTHH64 pKa = 6.15 IVPRR68 pKa = 11.84 PLPQTCKK75 pKa = 9.2 ATRR78 pKa = 11.84 VLKK81 pKa = 10.66 EE82 pKa = 3.5 FTNRR86 pKa = 11.84 MLLILLHH93 pKa = 6.05 RR94 pKa = 11.84 QPARR98 pKa = 11.84 SVSYY102 pKa = 10.64 ISQEE106 pKa = 3.96 CPMVGLVVVARR117 pKa = 11.84 LIYY120 pKa = 9.32 PIQKK124 pKa = 8.99 NQPNVHH130 pKa = 6.99 FGRR133 pKa = 11.84 NTTFMRR139 pKa = 11.84 KK140 pKa = 8.1 MNQEE144 pKa = 4.49 KK145 pKa = 9.24 ISKK148 pKa = 10.2 YY149 pKa = 8.82 IFRR152 pKa = 11.84 NKK154 pKa = 8.91 RR155 pKa = 11.84 TFVDD159 pKa = 3.76 PFKK162 pKa = 10.84 KK163 pKa = 9.23 TFTCRR168 pKa = 11.84 LKK170 pKa = 10.44 KK171 pKa = 10.25 AFKK174 pKa = 9.94 IFCVNCLCTRR184 pKa = 11.84 TARR187 pKa = 11.84 RR188 pKa = 11.84 THH190 pKa = 6.88 RR191 pKa = 11.84 APFQLPSFCCRR202 pKa = 11.84 QNKK205 pKa = 10.07 ASMTSLPAGRR215 pKa = 11.84 KK216 pKa = 8.46 YY217 pKa = 11.01 AVV219 pKa = 3.11
Molecular weight: 25.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.823
IPC_protein 10.672
Toseland 10.994
ProMoST 10.73
Dawson 11.052
Bjellqvist 10.789
Wikipedia 11.272
Rodwell 11.286
Grimsley 11.082
Solomon 11.213
Lehninger 11.184
Nozaki 10.994
DTASelect 10.774
Thurlkill 10.979
EMBOSS 11.403
Sillero 10.994
Patrickios 11.008
IPC_peptide 11.228
IPC2_peptide 10.131
IPC2.peptide.svr19 8.527
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
444
0
444
106395
47
1525
239.6
27.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.226 ± 0.112
2.398 ± 0.094
4.891 ± 0.078
8.265 ± 0.17
5.534 ± 0.093
6.15 ± 0.127
1.742 ± 0.063
5.162 ± 0.085
8.754 ± 0.206
8.836 ± 0.128
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.09 ± 0.054
4.113 ± 0.078
3.871 ± 0.106
3.506 ± 0.088
5.42 ± 0.093
7.523 ± 0.157
5.262 ± 0.157
6.56 ± 0.086
1.466 ± 0.047
3.23 ± 0.074
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here