Streptococcus phage IPP9

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5S7Y6|A0A1S5S7Y6_9CAUD Uncharacterized protein OS=Streptococcus phage IPP9 OX=1916207 GN=IPP9_00015 PE=4 SV=1
MM1 pKa = 6.48TQQVYY6 pKa = 9.6FEE8 pKa = 4.86SGLAVEE14 pKa = 4.77RR15 pKa = 11.84DD16 pKa = 3.38IYY18 pKa = 11.22CYY20 pKa = 10.95DD21 pKa = 3.57FDD23 pKa = 7.47DD24 pKa = 4.65IEE26 pKa = 4.99LMQSTGLKK34 pKa = 10.14DD35 pKa = 3.56KK36 pKa = 10.8NGKK39 pKa = 9.57EE40 pKa = 3.8IFEE43 pKa = 4.1GDD45 pKa = 2.94ILKK48 pKa = 10.86FNDD51 pKa = 3.03EE52 pKa = 4.11WNEE55 pKa = 3.91YY56 pKa = 8.78CHH58 pKa = 6.66EE59 pKa = 4.76GYY61 pKa = 10.91VDD63 pKa = 4.12GSVEE67 pKa = 3.79GVNYY71 pKa = 10.4VEE73 pKa = 4.35VAKK76 pKa = 11.2GEE78 pKa = 4.1ACFEE82 pKa = 4.35FGKK85 pKa = 8.67TRR87 pKa = 11.84YY88 pKa = 9.13PEE90 pKa = 4.12SSLFIYY96 pKa = 10.17MEE98 pKa = 4.56DD99 pKa = 3.22EE100 pKa = 4.15HH101 pKa = 8.96LSFAEE106 pKa = 4.08LVKK109 pKa = 11.17DD110 pKa = 3.37KK111 pKa = 11.45DD112 pKa = 3.72FGFEE116 pKa = 4.1IIDD119 pKa = 3.5NVYY122 pKa = 10.63EE123 pKa = 4.17NPEE126 pKa = 4.17LLEE129 pKa = 4.3VTHH132 pKa = 6.38EE133 pKa = 4.03

Molecular weight: 15.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5S7W2|A0A1S5S7W2_9CAUD Uncharacterized protein OS=Streptococcus phage IPP9 OX=1916207 GN=IPP9_00017 PE=4 SV=1
MM1 pKa = 7.81RR2 pKa = 11.84PRR4 pKa = 11.84RR5 pKa = 11.84YY6 pKa = 9.18PYY8 pKa = 10.05SGKK11 pKa = 10.15KK12 pKa = 9.81EE13 pKa = 4.19STFVKK18 pKa = 10.4ADD20 pKa = 3.56PEE22 pKa = 4.22LMLNINKK29 pKa = 9.29IDD31 pKa = 3.66VGNIQAKK38 pKa = 10.48NIFGKK43 pKa = 10.24II44 pKa = 3.35

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

12813

34

2083

203.4

23.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.93 ± 0.548

0.57 ± 0.099

6.267 ± 0.268

8.101 ± 0.483

4.012 ± 0.254

6.181 ± 0.553

1.428 ± 0.165

6.283 ± 0.278

8.882 ± 0.426

8.164 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.49 ± 0.206

5.416 ± 0.269

2.654 ± 0.238

4.066 ± 0.216

4.441 ± 0.316

6.056 ± 0.348

6.197 ± 0.363

6.423 ± 0.261

1.335 ± 0.162

4.105 ± 0.355
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski