Yersinia phage phiYeO3-12
Average proteome isoelectric point is 7.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9FZX9|Q9FZX9_9CAUD Putative 0.45 protein OS=Yersinia phage phiYeO3-12 OX=110457 GN=0.45 PE=4 SV=1
MM1 pKa = 7.73 IMPKK5 pKa = 10.08 SDD7 pKa = 3.6 TVTMTRR13 pKa = 11.84 DD14 pKa = 2.64 AWNDD18 pKa = 2.85 VSAYY22 pKa = 9.32 IDD24 pKa = 3.7 KK25 pKa = 11.04 LEE27 pKa = 4.85 KK28 pKa = 10.7 DD29 pKa = 4.0 LEE31 pKa = 4.14 FLNALKK37 pKa = 10.74 ACGVDD42 pKa = 3.06 NWDD45 pKa = 3.98 GYY47 pKa = 10.81 SDD49 pKa = 4.02 AVEE52 pKa = 4.1 MVYY55 pKa = 11.01 GEE57 pKa = 4.71 DD58 pKa = 4.8 DD59 pKa = 3.52 EE60 pKa = 5.21
Molecular weight: 6.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.144
IPC2_protein 3.795
IPC_protein 3.745
Toseland 3.528
ProMoST 3.884
Dawson 3.745
Bjellqvist 3.973
Wikipedia 3.706
Rodwell 3.567
Grimsley 3.439
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.605
EMBOSS 3.719
Sillero 3.872
Patrickios 3.35
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.808
Protein with the highest isoelectric point:
>tr|Q9T0Z4|Q9T0Z4_9CAUD Terminase large subunit OS=Yersinia phage phiYeO3-12 OX=110457 GN=19 PE=3 SV=1
MM1 pKa = 7.39 GTPQSSGLRR10 pKa = 11.84 SIRR13 pKa = 11.84 VAVRR17 pKa = 11.84 KK18 pKa = 7.11 TCTMATVWPRR28 pKa = 11.84 CSAKK32 pKa = 10.38 SSTMGSRR39 pKa = 11.84 CSKK42 pKa = 10.41 VNRR45 pKa = 11.84 LTPCASIWKK54 pKa = 7.91 TCVSVSWNTVRR65 pKa = 11.84 LASLCSSCSTRR76 pKa = 11.84 TT77 pKa = 3.28
Molecular weight: 8.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.292
IPC2_protein 9.56
IPC_protein 10.35
Toseland 11.052
ProMoST 11.008
Dawson 11.082
Bjellqvist 10.877
Wikipedia 11.345
Rodwell 11.199
Grimsley 11.096
Solomon 11.345
Lehninger 11.301
Nozaki 11.082
DTASelect 10.847
Thurlkill 11.038
EMBOSS 11.477
Sillero 11.052
Patrickios 10.994
IPC_peptide 11.359
IPC2_peptide 10.452
IPC2.peptide.svr19 9.108
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
59
0
59
13951
25
1320
236.5
26.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.354 ± 0.472
1.19 ± 0.229
6.107 ± 0.29
6.924 ± 0.335
3.512 ± 0.213
7.648 ± 0.321
2.014 ± 0.177
5.096 ± 0.176
6.645 ± 0.359
8.128 ± 0.313
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.874 ± 0.173
4.322 ± 0.313
3.598 ± 0.225
3.957 ± 0.3
5.964 ± 0.254
5.878 ± 0.345
5.512 ± 0.277
6.573 ± 0.272
1.555 ± 0.172
3.147 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here