Enterococcus phage EFAP-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C0M000|C0M000_9CAUD Terminase large subunit OS=Enterococcus phage EFAP-1 OX=627087 GN=EFAP1_gp20 PE=4 SV=1
MM1 pKa = 7.2QNAVWLVVIFYY12 pKa = 10.43VLGMLSLVTSMFFTGLVAGLVALAVALLIPSVILYY47 pKa = 10.26KK48 pKa = 10.7EE49 pKa = 4.13LGEE52 pKa = 4.6GEE54 pKa = 4.17

Molecular weight: 5.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C0M003|C0M003_9CAUD HNH endonuclease OS=Enterococcus phage EFAP-1 OX=627087 GN=EFAP1_gp23 PE=4 SV=1
MM1 pKa = 7.3VKK3 pKa = 10.27DD4 pKa = 3.82LHH6 pKa = 5.55TQQTRR11 pKa = 11.84AFKK14 pKa = 10.84SQTEE18 pKa = 3.57ADD20 pKa = 3.03KK21 pKa = 10.92FYY23 pKa = 10.87NKK25 pKa = 10.0KK26 pKa = 10.04SGYY29 pKa = 9.89FKK31 pKa = 10.78DD32 pKa = 3.44VRR34 pKa = 11.84TKK36 pKa = 9.85LGGRR40 pKa = 11.84NRR42 pKa = 11.84HH43 pKa = 4.73YY44 pKa = 10.75EE45 pKa = 3.97IIEE48 pKa = 4.19VVV50 pKa = 3.03

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

6325

50

1456

263.5

29.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.609 ± 0.51

0.395 ± 0.162

6.119 ± 0.389

7.32 ± 0.774

4.079 ± 0.25

7.526 ± 1.076

1.265 ± 0.225

6.435 ± 0.397

7.921 ± 0.53

7.953 ± 0.354

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.893 ± 0.266

6.071 ± 0.467

3.178 ± 0.339

4.253 ± 0.255

3.652 ± 0.291

6.15 ± 0.299

6.466 ± 0.561

7.036 ± 0.395

1.344 ± 0.157

3.336 ± 0.549
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski