Mobilicoccus pelagius NBRC 104925
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3112 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H5UW50|H5UW50_9MICO Putative ABC transporter permease/ATP-binding protein OS=Mobilicoccus pelagius NBRC 104925 OX=1089455 GN=MOPEL_135_01960 PE=4 SV=1
MM1 pKa = 7.9 SDD3 pKa = 3.07 GCGSGDD9 pKa = 3.24 GGGCGGCGGACGGPYY24 pKa = 9.97 VPEE27 pKa = 5.19 LDD29 pKa = 4.48 ARR31 pKa = 11.84 EE32 pKa = 4.57 LDD34 pKa = 3.46 PMIRR38 pKa = 11.84 QSAIFGVLMGLPPQGVVRR56 pKa = 11.84 IVVNSDD62 pKa = 2.97 PSGIADD68 pKa = 4.01 LLEE71 pKa = 4.13 EE72 pKa = 4.65 RR73 pKa = 11.84 LPGQYY78 pKa = 10.21 SADD81 pKa = 3.37 VDD83 pKa = 4.1 TVSEE87 pKa = 4.07 DD88 pKa = 3.28 EE89 pKa = 4.16 YY90 pKa = 11.32 RR91 pKa = 11.84 VLFSRR96 pKa = 11.84 AA97 pKa = 3.1
Molecular weight: 9.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.774
IPC2_protein 3.973
IPC_protein 3.91
Toseland 3.694
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.834
Rodwell 3.732
Grimsley 3.617
Solomon 3.872
Lehninger 3.834
Nozaki 4.024
DTASelect 4.228
Thurlkill 3.757
EMBOSS 3.846
Sillero 4.024
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.919
Protein with the highest isoelectric point:
>tr|H5USY4|H5USY4_9MICO Ribosomal RNA small subunit methyltransferase G OS=Mobilicoccus pelagius NBRC 104925 OX=1089455 GN=rsmG PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.16 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MSTRR26 pKa = 11.84 AGRR29 pKa = 11.84 SIIASRR35 pKa = 11.84 RR36 pKa = 11.84 LKK38 pKa = 10.57 GRR40 pKa = 11.84 KK41 pKa = 9.08 KK42 pKa = 9.61 LTAA45 pKa = 4.2
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3112
0
3112
1040358
29
1835
334.3
35.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.234 ± 0.061
0.767 ± 0.012
6.546 ± 0.041
5.802 ± 0.047
2.635 ± 0.024
9.365 ± 0.038
2.352 ± 0.02
3.291 ± 0.033
1.88 ± 0.037
9.836 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.925 ± 0.02
1.581 ± 0.021
6.054 ± 0.04
2.379 ± 0.024
8.158 ± 0.051
5.076 ± 0.03
6.503 ± 0.038
9.383 ± 0.045
1.412 ± 0.019
1.823 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here