Oscillatoria acuminata PCC 6304

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Oscillatoriaceae; Oscillatoria; Oscillatoria acuminata

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5755 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9TMM7|K9TMM7_9CYAN Site-specific DNA-methyltransferase (adenine-specific) OS=Oscillatoria acuminata PCC 6304 OX=56110 GN=Oscil6304_4139 PE=3 SV=1
MM1 pKa = 7.18TVFIALNDD9 pKa = 3.72NNTLVAFNANNPTQTSSVGVTGIEE33 pKa = 4.2GTLLGIDD40 pKa = 3.44TRR42 pKa = 11.84PANGLIYY49 pKa = 10.53GITTTNNIYY58 pKa = 10.77SIDD61 pKa = 3.88PQSLGFTIDD70 pKa = 3.98ANGALNNTMTPATVSATLVSTLSQPFEE97 pKa = 4.45GGTVSGVDD105 pKa = 3.99FNPVPDD111 pKa = 3.71RR112 pKa = 11.84LRR114 pKa = 11.84LVGEE118 pKa = 3.9NNQNFRR124 pKa = 11.84INVDD128 pKa = 3.17TGAVIVDD135 pKa = 3.86GTLAFAADD143 pKa = 4.23DD144 pKa = 4.15PNGAVNPTISASGYY158 pKa = 7.43TNSFAGTTSTQLYY171 pKa = 10.76NIDD174 pKa = 3.72TQLNTLLLQNPPNDD188 pKa = 3.77GTLVTIGNLGVDD200 pKa = 4.69FGNLAGFDD208 pKa = 3.52IVSGGMAGEE217 pKa = 3.99NAAFAVANSMLYY229 pKa = 10.63SIDD232 pKa = 3.59LTNGQASSLGMIGGNTGLNVQGLATVPTSTFRR264 pKa = 11.84DD265 pKa = 4.01TITGEE270 pKa = 4.36TEE272 pKa = 3.93FNPAQYY278 pKa = 10.38LASHH282 pKa = 7.79PDD284 pKa = 3.92LIAAFGYY291 pKa = 10.39NLAAAAQHH299 pKa = 6.07YY300 pKa = 10.93DD301 pKa = 3.37MFGMAEE307 pKa = 3.86NRR309 pKa = 11.84ALDD312 pKa = 3.49TFDD315 pKa = 3.25EE316 pKa = 4.27VRR318 pKa = 11.84YY319 pKa = 8.76LASYY323 pKa = 10.75ADD325 pKa = 5.24LIQVIGFNPEE335 pKa = 3.28LATEE339 pKa = 4.17HH340 pKa = 6.18FVVFGADD347 pKa = 3.1EE348 pKa = 4.33GRR350 pKa = 11.84VTNLFNPVNYY360 pKa = 10.28LNTYY364 pKa = 10.04ADD366 pKa = 3.39LRR368 pKa = 11.84AAFGNDD374 pKa = 2.71LMAATQHH381 pKa = 5.94YY382 pKa = 8.65IQNGFDD388 pKa = 3.38EE389 pKa = 4.95GRR391 pKa = 11.84VFF393 pKa = 4.87

Molecular weight: 41.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9TGF8|K9TGF8_9CYAN Quinolinate phosphoribosyltransferase [decarboxylating] OS=Oscillatoria acuminata PCC 6304 OX=56110 GN=Oscil6304_1800 PE=3 SV=1
MM1 pKa = 6.63TQRR4 pKa = 11.84TLHH7 pKa = 4.82GTSRR11 pKa = 11.84KK12 pKa = 8.94RR13 pKa = 11.84KK14 pKa = 7.72RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84TANGRR29 pKa = 11.84RR30 pKa = 11.84VIKK33 pKa = 10.2SRR35 pKa = 11.84RR36 pKa = 11.84NKK38 pKa = 9.36GRR40 pKa = 11.84YY41 pKa = 8.64RR42 pKa = 11.84LAVV45 pKa = 3.33

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5755

0

5755

2048953

23

3704

356.0

39.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.1 ± 0.032

0.97 ± 0.011

5.022 ± 0.03

6.881 ± 0.037

3.801 ± 0.02

7.114 ± 0.049

1.818 ± 0.015

6.373 ± 0.027

3.976 ± 0.033

11.058 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.896 ± 0.017

4.075 ± 0.029

5.295 ± 0.031

5.471 ± 0.033

5.441 ± 0.036

6.396 ± 0.024

5.719 ± 0.039

6.272 ± 0.026

1.467 ± 0.013

2.856 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski