Chitinophaga eiseniae
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6844 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T4U6K6|A0A1T4U6K6_9BACT Amino acid adenylation domain-containing protein (Fragment) OS=Chitinophaga eiseniae OX=634771 GN=SAMN04488128_11125 PE=4 SV=1
MM1 pKa = 7.87 YY2 pKa = 10.04 EE3 pKa = 4.47 IIPFKK8 pKa = 10.63 RR9 pKa = 11.84 VGQFEE14 pKa = 4.83 FNTSISRR21 pKa = 11.84 YY22 pKa = 9.76 LDD24 pKa = 3.28 GDD26 pKa = 3.5 NFNFYY31 pKa = 10.34 PKK33 pKa = 10.72 GDD35 pKa = 3.77 GEE37 pKa = 4.41 SWDD40 pKa = 3.76 TYY42 pKa = 9.64 SYY44 pKa = 10.98 RR45 pKa = 11.84 GGGIDD50 pKa = 4.27 IYY52 pKa = 10.63 TSDD55 pKa = 4.84 SIIEE59 pKa = 4.44 SISCRR64 pKa = 11.84 SDD66 pKa = 3.28 CFLEE70 pKa = 4.37 GQMLIGMNIEE80 pKa = 4.39 DD81 pKa = 4.23 FWAVFKK87 pKa = 10.9 IAVSNIKK94 pKa = 8.81 MEE96 pKa = 4.04 KK97 pKa = 10.25 VYY99 pKa = 10.56 FFDD102 pKa = 5.34 GSEE105 pKa = 3.85 QDD107 pKa = 4.49 VYY109 pKa = 11.69 DD110 pKa = 3.94 VDD112 pKa = 4.93 CLGLQLWVDD121 pKa = 3.6 QYY123 pKa = 10.62 NTIVTVFVSII133 pKa = 4.65
Molecular weight: 15.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.809
IPC2_protein 3.986
IPC_protein 3.948
Toseland 3.732
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.872
Rodwell 3.77
Grimsley 3.643
Solomon 3.923
Lehninger 3.884
Nozaki 4.05
DTASelect 4.279
Thurlkill 3.783
EMBOSS 3.884
Sillero 4.062
Patrickios 0.693
IPC_peptide 3.923
IPC2_peptide 4.037
IPC2.peptide.svr19 3.954
Protein with the highest isoelectric point:
>tr|A0A1T4U666|A0A1T4U666_9BACT Alkylated DNA repair dioxygenase AlkB OS=Chitinophaga eiseniae OX=634771 GN=SAMN04488128_1106 PE=4 SV=1
MM1 pKa = 7.54 PKK3 pKa = 9.97 VRR5 pKa = 11.84 THH7 pKa = 5.71 SRR9 pKa = 11.84 AKK11 pKa = 9.49 KK12 pKa = 7.2 TFKK15 pKa = 10.87 VGGNGQIKK23 pKa = 10.01 RR24 pKa = 11.84 FKK26 pKa = 10.59 AFKK29 pKa = 10.48 SHH31 pKa = 7.28 LLTKK35 pKa = 10.39 KK36 pKa = 7.92 ATKK39 pKa = 10.01 RR40 pKa = 11.84 KK41 pKa = 8.82 RR42 pKa = 11.84 SLRR45 pKa = 11.84 GSTLVHH51 pKa = 6.08 EE52 pKa = 5.21 ANLNLVKK59 pKa = 10.71 RR60 pKa = 11.84 MLGLRR65 pKa = 3.82
Molecular weight: 7.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.359
IPC2_protein 10.804
IPC_protein 12.325
Toseland 12.515
ProMoST 12.983
Dawson 12.515
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.457
Grimsley 12.544
Solomon 12.983
Lehninger 12.896
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.179
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.983
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6844
0
6844
2579896
29
8700
377.0
42.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.548 ± 0.035
0.852 ± 0.011
5.228 ± 0.018
5.273 ± 0.031
4.471 ± 0.023
7.121 ± 0.032
2.092 ± 0.018
6.15 ± 0.023
5.689 ± 0.033
9.711 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.45 ± 0.018
4.883 ± 0.035
4.396 ± 0.019
4.241 ± 0.02
4.829 ± 0.023
5.851 ± 0.025
6.11 ± 0.047
6.636 ± 0.027
1.318 ± 0.011
4.15 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here