Chitinophaga eiseniae 
Average proteome isoelectric point is 6.97 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 6844 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A1T4U6K6|A0A1T4U6K6_9BACT Amino acid adenylation domain-containing protein (Fragment) OS=Chitinophaga eiseniae OX=634771 GN=SAMN04488128_11125 PE=4 SV=1MM1 pKa = 7.87  YY2 pKa = 10.04  EE3 pKa = 4.47  IIPFKK8 pKa = 10.63  RR9 pKa = 11.84  VGQFEE14 pKa = 4.83  FNTSISRR21 pKa = 11.84  YY22 pKa = 9.76  LDD24 pKa = 3.28  GDD26 pKa = 3.5  NFNFYY31 pKa = 10.34  PKK33 pKa = 10.72  GDD35 pKa = 3.77  GEE37 pKa = 4.41  SWDD40 pKa = 3.76  TYY42 pKa = 9.64  SYY44 pKa = 10.98  RR45 pKa = 11.84  GGGIDD50 pKa = 4.27  IYY52 pKa = 10.63  TSDD55 pKa = 4.84  SIIEE59 pKa = 4.44  SISCRR64 pKa = 11.84  SDD66 pKa = 3.28  CFLEE70 pKa = 4.37  GQMLIGMNIEE80 pKa = 4.39  DD81 pKa = 4.23  FWAVFKK87 pKa = 10.9  IAVSNIKK94 pKa = 8.81  MEE96 pKa = 4.04  KK97 pKa = 10.25  VYY99 pKa = 10.56  FFDD102 pKa = 5.34  GSEE105 pKa = 3.85  QDD107 pKa = 4.49  VYY109 pKa = 11.69  DD110 pKa = 3.94  VDD112 pKa = 4.93  CLGLQLWVDD121 pKa = 3.6  QYY123 pKa = 10.62  NTIVTVFVSII133 pKa = 4.65  
 15.39 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.809 
IPC2_protein 3.986 
IPC_protein 3.948 
Toseland    3.732 
ProMoST     4.101 
Dawson      3.935 
Bjellqvist  4.088 
Wikipedia   3.872 
Rodwell     3.77 
Grimsley    3.643 
Solomon     3.923 
Lehninger   3.884 
Nozaki      4.05 
DTASelect   4.279 
Thurlkill   3.783 
EMBOSS      3.884 
Sillero     4.062 
Patrickios  0.693 
IPC_peptide 3.923 
IPC2_peptide  4.037 
IPC2.peptide.svr19  3.954 
 Protein with the highest isoelectric point: 
>tr|A0A1T4U666|A0A1T4U666_9BACT Alkylated DNA repair dioxygenase AlkB OS=Chitinophaga eiseniae OX=634771 GN=SAMN04488128_1106 PE=4 SV=1MM1 pKa = 7.54  PKK3 pKa = 9.97  VRR5 pKa = 11.84  THH7 pKa = 5.71  SRR9 pKa = 11.84  AKK11 pKa = 9.49  KK12 pKa = 7.2  TFKK15 pKa = 10.87  VGGNGQIKK23 pKa = 10.01  RR24 pKa = 11.84  FKK26 pKa = 10.59  AFKK29 pKa = 10.48  SHH31 pKa = 7.28  LLTKK35 pKa = 10.39  KK36 pKa = 7.92  ATKK39 pKa = 10.01  RR40 pKa = 11.84  KK41 pKa = 8.82  RR42 pKa = 11.84  SLRR45 pKa = 11.84  GSTLVHH51 pKa = 6.08  EE52 pKa = 5.21  ANLNLVKK59 pKa = 10.71  RR60 pKa = 11.84  MLGLRR65 pKa = 3.82  
 7.46 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.359 
IPC2_protein 10.804 
IPC_protein 12.325 
Toseland    12.515 
ProMoST     12.983 
Dawson      12.515 
Bjellqvist  12.486 
Wikipedia   12.969 
Rodwell     12.457 
Grimsley    12.544 
Solomon     12.983 
Lehninger   12.896 
Nozaki      12.501 
DTASelect   12.486 
Thurlkill   12.501 
EMBOSS      12.998 
Sillero     12.501 
Patrickios  12.179 
IPC_peptide 12.998 
IPC2_peptide  11.974 
IPC2.peptide.svr19  8.983 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        6844 
0
6844 
2579896
29
8700
377.0
42.1
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        8.548 ± 0.035
0.852 ± 0.011
5.228 ± 0.018
5.273 ± 0.031
4.471 ± 0.023
7.121 ± 0.032
2.092 ± 0.018
6.15 ± 0.023
5.689 ± 0.033
9.711 ± 0.041
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.45 ± 0.018
4.883 ± 0.035
4.396 ± 0.019
4.241 ± 0.02
4.829 ± 0.023
5.851 ± 0.025
6.11 ± 0.047
6.636 ± 0.027
1.318 ± 0.011
4.15 ± 0.021
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here