Hubei narna-like virus 22

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KIM3|A0A1L3KIM3_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 22 OX=1922953 PE=4 SV=1
MM1 pKa = 7.58EE2 pKa = 6.53KK3 pKa = 10.29DD4 pKa = 5.69LKK6 pKa = 10.76MKK8 pKa = 8.65WQQRR12 pKa = 11.84EE13 pKa = 3.99WFNPSHH19 pKa = 6.25LTGSSIPGSGKK30 pKa = 10.49AMSIRR35 pKa = 11.84NRR37 pKa = 11.84NGNISYY43 pKa = 10.95ASIVSAYY50 pKa = 10.42SFSLSTAPLFIWKK63 pKa = 9.46FLDD66 pKa = 4.36DD67 pKa = 3.49IKK69 pKa = 10.87YY70 pKa = 9.71PEE72 pKa = 4.25MVYY75 pKa = 10.18EE76 pKa = 4.07VQGNLEE82 pKa = 3.94QSIKK86 pKa = 10.3LSQIIEE92 pKa = 3.9EE93 pKa = 4.09RR94 pKa = 11.84LQNEE98 pKa = 4.15RR99 pKa = 11.84LLDD102 pKa = 3.84EE103 pKa = 4.98ALSGNNFLDD112 pKa = 4.36DD113 pKa = 4.69DD114 pKa = 4.92SFVDD118 pKa = 3.58YY119 pKa = 11.15QKK121 pKa = 10.93FVTPILGLHH130 pKa = 6.28HH131 pKa = 6.75SVGNIGFLQQGSGKK145 pKa = 9.72LRR147 pKa = 11.84AVANPNRR154 pKa = 11.84FVQYY158 pKa = 10.26CNSPLGEE165 pKa = 4.24VLSSHH170 pKa = 5.58QQGLDD175 pKa = 2.93GCFVYY180 pKa = 10.64NQEE183 pKa = 5.88AGIEE187 pKa = 4.13WVKK190 pKa = 11.01RR191 pKa = 11.84KK192 pKa = 9.74LKK194 pKa = 10.35QGNTLASFDD203 pKa = 3.75MSAATDD209 pKa = 3.29RR210 pKa = 11.84LDD212 pKa = 3.33FQKK215 pKa = 10.84FINEE219 pKa = 3.95YY220 pKa = 9.53FVNISDD226 pKa = 3.74EE227 pKa = 4.17RR228 pKa = 11.84HH229 pKa = 5.83PLLSRR234 pKa = 11.84SIEE237 pKa = 4.13LFKK240 pKa = 10.79DD241 pKa = 3.51TSLSPWSLPGFVADD255 pKa = 4.02MLQMPSNEE263 pKa = 4.03ISWKK267 pKa = 9.82IGQPLGLRR275 pKa = 11.84PSFPLLTIMNCAMASQAVRR294 pKa = 11.84NLDD297 pKa = 3.32GYY299 pKa = 9.15GTYY302 pKa = 8.93NTFACVGDD310 pKa = 4.05DD311 pKa = 4.14LVIQAEE317 pKa = 4.23YY318 pKa = 11.0ADD320 pKa = 4.99AYY322 pKa = 8.82VQVVSEE328 pKa = 4.37FSGLINRR335 pKa = 11.84DD336 pKa = 2.98KK337 pKa = 11.6GMTSSKK343 pKa = 10.32YY344 pKa = 10.79AEE346 pKa = 4.51FCSQLITADD355 pKa = 2.9SSYY358 pKa = 11.38ALKK361 pKa = 10.37PRR363 pKa = 11.84WIDD366 pKa = 3.61SIEE369 pKa = 4.02GSLNNIEE376 pKa = 4.5KK377 pKa = 10.11FQTSGLHH384 pKa = 5.53PKK386 pKa = 9.52VPEE389 pKa = 4.56WIQRR393 pKa = 11.84LYY395 pKa = 10.92NAIAKK400 pKa = 10.04RR401 pKa = 11.84SIKK404 pKa = 10.64GFTKK408 pKa = 10.53YY409 pKa = 10.79GYY411 pKa = 8.5TKK413 pKa = 9.78TDD415 pKa = 3.73CYY417 pKa = 11.48DD418 pKa = 3.42SLKK421 pKa = 11.04DD422 pKa = 3.36RR423 pKa = 11.84VAIHH427 pKa = 7.15DD428 pKa = 4.85FIQSTRR434 pKa = 11.84KK435 pKa = 9.38APRR438 pKa = 11.84DD439 pKa = 3.33IEE441 pKa = 4.79EE442 pKa = 4.01ITLQTLYY449 pKa = 9.94MRR451 pKa = 11.84AVSEE455 pKa = 4.32QDD457 pKa = 3.2EE458 pKa = 4.63LNQKK462 pKa = 8.05VKK464 pKa = 10.84CFDD467 pKa = 4.8LSTSWAMPKK476 pKa = 10.36LGQNKK481 pKa = 8.6QEE483 pKa = 3.54ISTRR487 pKa = 11.84HH488 pKa = 5.62KK489 pKa = 11.03DD490 pKa = 2.94IVQKK494 pKa = 11.06YY495 pKa = 9.57DD496 pKa = 3.16IEE498 pKa = 6.22LGDD501 pKa = 4.35LPSDD505 pKa = 3.52RR506 pKa = 11.84ATSVAVPGIQQWNYY520 pKa = 10.97KK521 pKa = 8.18EE522 pKa = 5.08DD523 pKa = 3.92CYY525 pKa = 11.3SPKK528 pKa = 10.19VSEE531 pKa = 4.71LNTAKK536 pKa = 10.84KK537 pKa = 10.02LLKK540 pKa = 9.46TIEE543 pKa = 4.33SIKK546 pKa = 10.57VEE548 pKa = 4.15SAGTLISASQEE559 pKa = 4.03TKK561 pKa = 9.89HH562 pKa = 6.31CRR564 pKa = 11.84TEE566 pKa = 3.64ILTDD570 pKa = 4.06LSNHH574 pKa = 5.96EE575 pKa = 4.36SVIVHH580 pKa = 5.85TNKK583 pKa = 9.09ATNTTAIQTKK593 pKa = 9.81HH594 pKa = 4.41NQKK597 pKa = 10.34SKK599 pKa = 10.76RR600 pKa = 11.84LEE602 pKa = 4.12DD603 pKa = 3.54LLANLKK609 pKa = 9.93IDD611 pKa = 4.47DD612 pKa = 4.63PTDD615 pKa = 3.35EE616 pKa = 5.11AQTSMEE622 pKa = 3.87TSYY625 pKa = 11.91

Molecular weight: 70.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KIM3|A0A1L3KIM3_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 22 OX=1922953 PE=4 SV=1
MM1 pKa = 7.58EE2 pKa = 6.53KK3 pKa = 10.29DD4 pKa = 5.69LKK6 pKa = 10.76MKK8 pKa = 8.65WQQRR12 pKa = 11.84EE13 pKa = 3.99WFNPSHH19 pKa = 6.25LTGSSIPGSGKK30 pKa = 10.49AMSIRR35 pKa = 11.84NRR37 pKa = 11.84NGNISYY43 pKa = 10.95ASIVSAYY50 pKa = 10.42SFSLSTAPLFIWKK63 pKa = 9.46FLDD66 pKa = 4.36DD67 pKa = 3.49IKK69 pKa = 10.87YY70 pKa = 9.71PEE72 pKa = 4.25MVYY75 pKa = 10.18EE76 pKa = 4.07VQGNLEE82 pKa = 3.94QSIKK86 pKa = 10.3LSQIIEE92 pKa = 3.9EE93 pKa = 4.09RR94 pKa = 11.84LQNEE98 pKa = 4.15RR99 pKa = 11.84LLDD102 pKa = 3.84EE103 pKa = 4.98ALSGNNFLDD112 pKa = 4.36DD113 pKa = 4.69DD114 pKa = 4.92SFVDD118 pKa = 3.58YY119 pKa = 11.15QKK121 pKa = 10.93FVTPILGLHH130 pKa = 6.28HH131 pKa = 6.75SVGNIGFLQQGSGKK145 pKa = 9.72LRR147 pKa = 11.84AVANPNRR154 pKa = 11.84FVQYY158 pKa = 10.26CNSPLGEE165 pKa = 4.24VLSSHH170 pKa = 5.58QQGLDD175 pKa = 2.93GCFVYY180 pKa = 10.64NQEE183 pKa = 5.88AGIEE187 pKa = 4.13WVKK190 pKa = 11.01RR191 pKa = 11.84KK192 pKa = 9.74LKK194 pKa = 10.35QGNTLASFDD203 pKa = 3.75MSAATDD209 pKa = 3.29RR210 pKa = 11.84LDD212 pKa = 3.33FQKK215 pKa = 10.84FINEE219 pKa = 3.95YY220 pKa = 9.53FVNISDD226 pKa = 3.74EE227 pKa = 4.17RR228 pKa = 11.84HH229 pKa = 5.83PLLSRR234 pKa = 11.84SIEE237 pKa = 4.13LFKK240 pKa = 10.79DD241 pKa = 3.51TSLSPWSLPGFVADD255 pKa = 4.02MLQMPSNEE263 pKa = 4.03ISWKK267 pKa = 9.82IGQPLGLRR275 pKa = 11.84PSFPLLTIMNCAMASQAVRR294 pKa = 11.84NLDD297 pKa = 3.32GYY299 pKa = 9.15GTYY302 pKa = 8.93NTFACVGDD310 pKa = 4.05DD311 pKa = 4.14LVIQAEE317 pKa = 4.23YY318 pKa = 11.0ADD320 pKa = 4.99AYY322 pKa = 8.82VQVVSEE328 pKa = 4.37FSGLINRR335 pKa = 11.84DD336 pKa = 2.98KK337 pKa = 11.6GMTSSKK343 pKa = 10.32YY344 pKa = 10.79AEE346 pKa = 4.51FCSQLITADD355 pKa = 2.9SSYY358 pKa = 11.38ALKK361 pKa = 10.37PRR363 pKa = 11.84WIDD366 pKa = 3.61SIEE369 pKa = 4.02GSLNNIEE376 pKa = 4.5KK377 pKa = 10.11FQTSGLHH384 pKa = 5.53PKK386 pKa = 9.52VPEE389 pKa = 4.56WIQRR393 pKa = 11.84LYY395 pKa = 10.92NAIAKK400 pKa = 10.04RR401 pKa = 11.84SIKK404 pKa = 10.64GFTKK408 pKa = 10.53YY409 pKa = 10.79GYY411 pKa = 8.5TKK413 pKa = 9.78TDD415 pKa = 3.73CYY417 pKa = 11.48DD418 pKa = 3.42SLKK421 pKa = 11.04DD422 pKa = 3.36RR423 pKa = 11.84VAIHH427 pKa = 7.15DD428 pKa = 4.85FIQSTRR434 pKa = 11.84KK435 pKa = 9.38APRR438 pKa = 11.84DD439 pKa = 3.33IEE441 pKa = 4.79EE442 pKa = 4.01ITLQTLYY449 pKa = 9.94MRR451 pKa = 11.84AVSEE455 pKa = 4.32QDD457 pKa = 3.2EE458 pKa = 4.63LNQKK462 pKa = 8.05VKK464 pKa = 10.84CFDD467 pKa = 4.8LSTSWAMPKK476 pKa = 10.36LGQNKK481 pKa = 8.6QEE483 pKa = 3.54ISTRR487 pKa = 11.84HH488 pKa = 5.62KK489 pKa = 11.03DD490 pKa = 2.94IVQKK494 pKa = 11.06YY495 pKa = 9.57DD496 pKa = 3.16IEE498 pKa = 6.22LGDD501 pKa = 4.35LPSDD505 pKa = 3.52RR506 pKa = 11.84ATSVAVPGIQQWNYY520 pKa = 10.97KK521 pKa = 8.18EE522 pKa = 5.08DD523 pKa = 3.92CYY525 pKa = 11.3SPKK528 pKa = 10.19VSEE531 pKa = 4.71LNTAKK536 pKa = 10.84KK537 pKa = 10.02LLKK540 pKa = 9.46TIEE543 pKa = 4.33SIKK546 pKa = 10.57VEE548 pKa = 4.15SAGTLISASQEE559 pKa = 4.03TKK561 pKa = 9.89HH562 pKa = 6.31CRR564 pKa = 11.84TEE566 pKa = 3.64ILTDD570 pKa = 4.06LSNHH574 pKa = 5.96EE575 pKa = 4.36SVIVHH580 pKa = 5.85TNKK583 pKa = 9.09ATNTTAIQTKK593 pKa = 9.81HH594 pKa = 4.41NQKK597 pKa = 10.34SKK599 pKa = 10.76RR600 pKa = 11.84LEE602 pKa = 4.12DD603 pKa = 3.54LLANLKK609 pKa = 9.93IDD611 pKa = 4.47DD612 pKa = 4.63PTDD615 pKa = 3.35EE616 pKa = 5.11AQTSMEE622 pKa = 3.87TSYY625 pKa = 11.91

Molecular weight: 70.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

625

625

625

625.0

70.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.92 ± 0.0

1.44 ± 0.0

6.24 ± 0.0

6.08 ± 0.0

3.84 ± 0.0

5.12 ± 0.0

1.92 ± 0.0

6.72 ± 0.0

7.04 ± 0.0

9.28 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.08 ± 0.0

5.28 ± 0.0

3.68 ± 0.0

5.76 ± 0.0

4.0 ± 0.0

9.92 ± 0.0

5.6 ± 0.0

4.8 ± 0.0

1.6 ± 0.0

3.68 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski