Amino acid dipepetide frequency for Bacillus phage Anath

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.0AlaAla: 0.0 ± 0.0
0.697AlaCys: 0.697 ± 0.214
4.053AlaAsp: 4.053 ± 0.564
4.75AlaGlu: 4.75 ± 0.551
3.42AlaPhe: 3.42 ± 0.449
6.777AlaGly: 6.777 ± 0.839
1.583AlaHis: 1.583 ± 0.35
5.257AlaIle: 5.257 ± 0.572
5.383AlaLys: 5.383 ± 0.625
6.08AlaLeu: 6.08 ± 0.677
2.407AlaMet: 2.407 ± 0.449
3.103AlaAsn: 3.103 ± 0.474
2.217AlaPro: 2.217 ± 0.369
2.47AlaGln: 2.47 ± 0.437
3.863AlaArg: 3.863 ± 0.534
4.687AlaSer: 4.687 ± 0.65
4.433AlaThr: 4.433 ± 0.595
5.447AlaVal: 5.447 ± 0.869
1.077AlaTrp: 1.077 ± 0.291
2.66AlaTyr: 2.66 ± 0.473
0.0AlaXaa: 0.0 ± 0.0
Cys
0.507CysAla: 0.507 ± 0.143
0.19CysCys: 0.19 ± 0.174
0.443CysAsp: 0.443 ± 0.168
1.013CysGlu: 1.013 ± 0.269
0.697CysPhe: 0.697 ± 0.221
0.443CysGly: 0.443 ± 0.145
0.38CysHis: 0.38 ± 0.149
0.57CysIle: 0.57 ± 0.188
0.633CysLys: 0.633 ± 0.226
0.697CysLeu: 0.697 ± 0.271
0.127CysMet: 0.127 ± 0.094
0.633CysAsn: 0.633 ± 0.185
0.253CysPro: 0.253 ± 0.142
0.317CysGln: 0.317 ± 0.142
0.38CysArg: 0.38 ± 0.138
0.507CysSer: 0.507 ± 0.194
0.507CysThr: 0.507 ± 0.185
0.507CysVal: 0.507 ± 0.245
0.0CysTrp: 0.0 ± 0.0
0.38CysTyr: 0.38 ± 0.137
0.0CysXaa: 0.0 ± 0.0
Asp
3.61AspAla: 3.61 ± 0.479
0.57AspCys: 0.57 ± 0.195
2.217AspAsp: 2.217 ± 0.432
4.307AspGlu: 4.307 ± 0.638
3.04AspPhe: 3.04 ± 0.464
4.37AspGly: 4.37 ± 0.581
1.14AspHis: 1.14 ± 0.336
3.23AspIle: 3.23 ± 0.724
5.827AspLys: 5.827 ± 0.609
4.243AspLeu: 4.243 ± 0.563
1.9AspMet: 1.9 ± 0.395
2.913AspAsn: 2.913 ± 0.448
1.773AspPro: 1.773 ± 0.383
1.583AspGln: 1.583 ± 0.32
2.85AspArg: 2.85 ± 0.432
3.8AspSer: 3.8 ± 0.46
3.23AspThr: 3.23 ± 0.386
4.56AspVal: 4.56 ± 0.567
0.76AspTrp: 0.76 ± 0.183
3.103AspTyr: 3.103 ± 0.384
0.0AspXaa: 0.0 ± 0.0
Glu
5.13GluAla: 5.13 ± 0.537
0.95GluCys: 0.95 ± 0.27
3.737GluAsp: 3.737 ± 0.538
6.587GluGlu: 6.587 ± 0.809
3.293GluPhe: 3.293 ± 0.42
5.51GluGly: 5.51 ± 0.63
1.71GluHis: 1.71 ± 0.402
4.687GluIle: 4.687 ± 0.617
5.827GluLys: 5.827 ± 0.692
7.79GluLeu: 7.79 ± 0.657
2.217GluMet: 2.217 ± 0.362
2.85GluAsn: 2.85 ± 0.426
0.887GluPro: 0.887 ± 0.217
3.737GluGln: 3.737 ± 0.47
4.243GluArg: 4.243 ± 0.595
2.85GluSer: 2.85 ± 0.405
3.737GluThr: 3.737 ± 0.521
6.397GluVal: 6.397 ± 0.755
1.33GluTrp: 1.33 ± 0.38
2.66GluTyr: 2.66 ± 0.359
0.0GluXaa: 0.0 ± 0.0
Phe
2.723PheAla: 2.723 ± 0.462
0.507PheCys: 0.507 ± 0.171
3.04PheAsp: 3.04 ± 0.459
3.483PheGlu: 3.483 ± 0.376
1.52PhePhe: 1.52 ± 0.295
2.597PheGly: 2.597 ± 0.408
1.013PheHis: 1.013 ± 0.257
2.217PheIle: 2.217 ± 0.445
3.547PheLys: 3.547 ± 0.44
3.04PheLeu: 3.04 ± 0.36
1.457PheMet: 1.457 ± 0.317
2.407PheAsn: 2.407 ± 0.299
1.647PhePro: 1.647 ± 0.34
1.773PheGln: 1.773 ± 0.347
1.393PheArg: 1.393 ± 0.275
3.04PheSer: 3.04 ± 0.367
3.357PheThr: 3.357 ± 0.371
3.167PheVal: 3.167 ± 0.39
0.507PheTrp: 0.507 ± 0.146
1.71PheTyr: 1.71 ± 0.377
0.0PheXaa: 0.0 ± 0.0
Gly
4.813GlyAla: 4.813 ± 0.572
0.887GlyCys: 0.887 ± 0.261
3.99GlyAsp: 3.99 ± 0.536
5.193GlyGlu: 5.193 ± 0.55
3.357GlyPhe: 3.357 ± 0.391
5.13GlyGly: 5.13 ± 0.547
1.013GlyHis: 1.013 ± 0.277
3.863GlyIle: 3.863 ± 0.564
5.51GlyLys: 5.51 ± 0.696
5.574GlyLeu: 5.574 ± 0.704
2.027GlyMet: 2.027 ± 0.347
3.04GlyAsn: 3.04 ± 0.511
0.127GlyPro: 0.127 ± 0.079
1.9GlyGln: 1.9 ± 0.501
3.103GlyArg: 3.103 ± 0.534
3.483GlySer: 3.483 ± 0.496
4.117GlyThr: 4.117 ± 0.524
5.637GlyVal: 5.637 ± 0.599
1.393GlyTrp: 1.393 ± 0.355
3.167GlyTyr: 3.167 ± 0.458
0.0GlyXaa: 0.0 ± 0.0
His
1.013HisAla: 1.013 ± 0.241
0.19HisCys: 0.19 ± 0.119
0.95HisAsp: 0.95 ± 0.243
1.9HisGlu: 1.9 ± 0.419
1.203HisPhe: 1.203 ± 0.304
1.583HisGly: 1.583 ± 0.331
0.507HisHis: 0.507 ± 0.162
1.393HisIle: 1.393 ± 0.273
1.013HisLys: 1.013 ± 0.242
1.583HisLeu: 1.583 ± 0.301
0.38HisMet: 0.38 ± 0.146
1.71HisAsn: 1.71 ± 0.335
0.633HisPro: 0.633 ± 0.169
0.95HisGln: 0.95 ± 0.228
0.95HisArg: 0.95 ± 0.257
0.823HisSer: 0.823 ± 0.19
1.077HisThr: 1.077 ± 0.254
1.013HisVal: 1.013 ± 0.259
0.127HisTrp: 0.127 ± 0.088
0.633HisTyr: 0.633 ± 0.193
0.0HisXaa: 0.0 ± 0.0
Ile
5.32IleAla: 5.32 ± 0.496
0.697IleCys: 0.697 ± 0.274
5.13IleAsp: 5.13 ± 0.577
4.94IleGlu: 4.94 ± 0.458
1.963IlePhe: 1.963 ± 0.299
3.99IleGly: 3.99 ± 0.643
0.95IleHis: 0.95 ± 0.267
3.483IleIle: 3.483 ± 0.63
5.447IleLys: 5.447 ± 0.603
3.42IleLeu: 3.42 ± 0.428
0.95IleMet: 0.95 ± 0.202
3.357IleAsn: 3.357 ± 0.476
2.407IlePro: 2.407 ± 0.424
3.04IleGln: 3.04 ± 0.296
3.23IleArg: 3.23 ± 0.489
3.04IleSer: 3.04 ± 0.54
3.61IleThr: 3.61 ± 0.414
4.053IleVal: 4.053 ± 0.521
0.317IleTrp: 0.317 ± 0.136
2.027IleTyr: 2.027 ± 0.365
0.0IleXaa: 0.0 ± 0.0
Lys
7.917LysAla: 7.917 ± 0.715
0.317LysCys: 0.317 ± 0.168
4.243LysAsp: 4.243 ± 0.524
5.13LysGlu: 5.13 ± 0.799
3.99LysPhe: 3.99 ± 0.615
4.75LysGly: 4.75 ± 0.562
1.837LysHis: 1.837 ± 0.322
4.117LysIle: 4.117 ± 0.722
6.27LysLys: 6.27 ± 0.895
6.334LysLeu: 6.334 ± 0.582
3.103LysMet: 3.103 ± 0.43
2.66LysAsn: 2.66 ± 0.367
1.9LysPro: 1.9 ± 0.373
3.483LysGln: 3.483 ± 0.391
4.433LysArg: 4.433 ± 0.582
4.053LysSer: 4.053 ± 0.541
3.673LysThr: 3.673 ± 0.505
6.46LysVal: 6.46 ± 0.737
0.887LysTrp: 0.887 ± 0.214
2.66LysTyr: 2.66 ± 0.483
0.0LysXaa: 0.0 ± 0.0
Leu
5.637LeuAla: 5.637 ± 0.697
1.013LeuCys: 1.013 ± 0.237
5.7LeuAsp: 5.7 ± 0.506
6.207LeuGlu: 6.207 ± 0.763
3.103LeuPhe: 3.103 ± 0.447
5.954LeuGly: 5.954 ± 0.605
1.773LeuHis: 1.773 ± 0.367
4.813LeuIle: 4.813 ± 0.602
5.954LeuLys: 5.954 ± 0.734
6.27LeuLeu: 6.27 ± 0.696
2.153LeuMet: 2.153 ± 0.448
4.053LeuAsn: 4.053 ± 0.512
2.407LeuPro: 2.407 ± 0.351
3.547LeuGln: 3.547 ± 0.442
3.673LeuArg: 3.673 ± 0.448
4.75LeuSer: 4.75 ± 0.539
5.067LeuThr: 5.067 ± 0.658
4.56LeuVal: 4.56 ± 0.58
1.013LeuTrp: 1.013 ± 0.426
3.103LeuTyr: 3.103 ± 0.42
0.0LeuXaa: 0.0 ± 0.0
Met
2.533MetAla: 2.533 ± 0.506
0.127MetCys: 0.127 ± 0.085
1.52MetAsp: 1.52 ± 0.289
1.647MetGlu: 1.647 ± 0.338
1.33MetPhe: 1.33 ± 0.257
2.027MetGly: 2.027 ± 0.304
0.507MetHis: 0.507 ± 0.173
1.9MetIle: 1.9 ± 0.384
2.28MetLys: 2.28 ± 0.377
1.963MetLeu: 1.963 ± 0.409
0.887MetMet: 0.887 ± 0.226
2.28MetAsn: 2.28 ± 0.414
0.76MetPro: 0.76 ± 0.245
1.33MetGln: 1.33 ± 0.279
1.52MetArg: 1.52 ± 0.273
2.343MetSer: 2.343 ± 0.371
2.47MetThr: 2.47 ± 0.383
1.457MetVal: 1.457 ± 0.334
0.823MetTrp: 0.823 ± 0.252
0.823MetTyr: 0.823 ± 0.25
0.0MetXaa: 0.0 ± 0.0
Asn
3.61AsnAla: 3.61 ± 0.582
0.443AsnCys: 0.443 ± 0.155
2.977AsnAsp: 2.977 ± 0.33
3.167AsnGlu: 3.167 ± 0.51
2.407AsnPhe: 2.407 ± 0.365
4.243AsnGly: 4.243 ± 0.45
1.013AsnHis: 1.013 ± 0.271
2.66AsnIle: 2.66 ± 0.536
3.42AsnLys: 3.42 ± 0.485
4.18AsnLeu: 4.18 ± 0.471
1.583AsnMet: 1.583 ± 0.285
2.85AsnAsn: 2.85 ± 0.382
1.71AsnPro: 1.71 ± 0.337
1.393AsnGln: 1.393 ± 0.273
2.09AsnArg: 2.09 ± 0.334
3.23AsnSer: 3.23 ± 0.629
2.977AsnThr: 2.977 ± 0.55
3.863AsnVal: 3.863 ± 0.544
1.077AsnTrp: 1.077 ± 0.283
1.647AsnTyr: 1.647 ± 0.391
0.0AsnXaa: 0.0 ± 0.0
Pro
2.977ProAla: 2.977 ± 0.525
0.063ProCys: 0.063 ± 0.058
1.647ProAsp: 1.647 ± 0.399
2.28ProGlu: 2.28 ± 0.437
1.457ProPhe: 1.457 ± 0.281
0.063ProGly: 0.063 ± 0.059
0.76ProHis: 0.76 ± 0.195
2.09ProIle: 2.09 ± 0.348
1.773ProLys: 1.773 ± 0.406
2.597ProLeu: 2.597 ± 0.466
0.887ProMet: 0.887 ± 0.217
1.647ProAsn: 1.647 ± 0.356
1.14ProPro: 1.14 ± 0.26
1.393ProGln: 1.393 ± 0.268
0.887ProArg: 0.887 ± 0.217
1.393ProSer: 1.393 ± 0.353
2.153ProThr: 2.153 ± 0.41
1.773ProVal: 1.773 ± 0.373
0.57ProTrp: 0.57 ± 0.142
1.14ProTyr: 1.14 ± 0.251
0.0ProXaa: 0.0 ± 0.0
Gln
3.23GlnAla: 3.23 ± 0.476
0.19GlnCys: 0.19 ± 0.116
2.153GlnAsp: 2.153 ± 0.356
2.977GlnGlu: 2.977 ± 0.401
1.773GlnPhe: 1.773 ± 0.269
2.85GlnGly: 2.85 ± 0.417
0.95GlnHis: 0.95 ± 0.249
1.52GlnIle: 1.52 ± 0.33
3.103GlnLys: 3.103 ± 0.418
4.37GlnLeu: 4.37 ± 0.513
1.33GlnMet: 1.33 ± 0.31
1.52GlnAsn: 1.52 ± 0.276
1.203GlnPro: 1.203 ± 0.323
1.14GlnGln: 1.14 ± 0.223
2.09GlnArg: 2.09 ± 0.347
1.9GlnSer: 1.9 ± 0.366
1.963GlnThr: 1.963 ± 0.308
3.23GlnVal: 3.23 ± 0.523
0.253GlnTrp: 0.253 ± 0.104
1.267GlnTyr: 1.267 ± 0.245
0.0GlnXaa: 0.0 ± 0.0
Arg
3.61ArgAla: 3.61 ± 0.388
0.38ArgCys: 0.38 ± 0.155
2.09ArgAsp: 2.09 ± 0.419
3.927ArgGlu: 3.927 ± 0.514
1.963ArgPhe: 1.963 ± 0.363
2.343ArgGly: 2.343 ± 0.379
0.697ArgHis: 0.697 ± 0.222
3.04ArgIle: 3.04 ± 0.378
4.75ArgLys: 4.75 ± 0.52
3.863ArgLeu: 3.863 ± 0.458
1.773ArgMet: 1.773 ± 0.351
2.66ArgAsn: 2.66 ± 0.423
1.203ArgPro: 1.203 ± 0.291
1.583ArgGln: 1.583 ± 0.352
1.52ArgArg: 1.52 ± 0.295
2.027ArgSer: 2.027 ± 0.318
3.04ArgThr: 3.04 ± 0.499
2.217ArgVal: 2.217 ± 0.376
0.57ArgTrp: 0.57 ± 0.167
2.343ArgTyr: 2.343 ± 0.422
0.0ArgXaa: 0.0 ± 0.0
Ser
4.56SerAla: 4.56 ± 0.802
0.57SerCys: 0.57 ± 0.201
2.977SerAsp: 2.977 ± 0.48
3.927SerGlu: 3.927 ± 0.412
2.153SerPhe: 2.153 ± 0.474
3.293SerGly: 3.293 ± 0.512
1.013SerHis: 1.013 ± 0.377
3.673SerIle: 3.673 ± 0.588
4.307SerLys: 4.307 ± 0.564
4.18SerLeu: 4.18 ± 0.47
1.963SerMet: 1.963 ± 0.289
3.103SerAsn: 3.103 ± 0.53
1.963SerPro: 1.963 ± 0.413
2.533SerGln: 2.533 ± 0.342
1.267SerArg: 1.267 ± 0.247
2.85SerSer: 2.85 ± 0.656
3.863SerThr: 3.863 ± 0.874
3.8SerVal: 3.8 ± 0.553
1.203SerTrp: 1.203 ± 0.28
1.9SerTyr: 1.9 ± 0.415
0.0SerXaa: 0.0 ± 0.0
Thr
4.243ThrAla: 4.243 ± 0.63
0.253ThrCys: 0.253 ± 0.165
3.8ThrAsp: 3.8 ± 0.335
4.497ThrGlu: 4.497 ± 0.491
2.597ThrPhe: 2.597 ± 0.337
3.547ThrGly: 3.547 ± 0.453
0.76ThrHis: 0.76 ± 0.207
3.863ThrIle: 3.863 ± 0.527
3.927ThrLys: 3.927 ± 0.505
5.003ThrLeu: 5.003 ± 0.478
1.963ThrMet: 1.963 ± 0.293
3.167ThrAsn: 3.167 ± 0.42
3.167ThrPro: 3.167 ± 0.395
2.153ThrGln: 2.153 ± 0.401
2.09ThrArg: 2.09 ± 0.276
2.597ThrSer: 2.597 ± 0.481
3.547ThrThr: 3.547 ± 0.495
5.193ThrVal: 5.193 ± 0.725
1.013ThrTrp: 1.013 ± 0.352
2.597ThrTyr: 2.597 ± 0.34
0.0ThrXaa: 0.0 ± 0.0
Val
6.27ValAla: 6.27 ± 0.591
0.633ValCys: 0.633 ± 0.163
4.813ValAsp: 4.813 ± 0.436
5.574ValGlu: 5.574 ± 0.613
2.343ValPhe: 2.343 ± 0.392
4.37ValGly: 4.37 ± 0.572
0.823ValHis: 0.823 ± 0.243
5.32ValIle: 5.32 ± 0.664
5.89ValLys: 5.89 ± 0.652
5.32ValLeu: 5.32 ± 0.673
1.203ValMet: 1.203 ± 0.285
3.04ValAsn: 3.04 ± 0.513
2.47ValPro: 2.47 ± 0.374
2.47ValGln: 2.47 ± 0.355
3.673ValArg: 3.673 ± 0.449
4.117ValSer: 4.117 ± 0.666
3.8ValThr: 3.8 ± 0.547
4.877ValVal: 4.877 ± 0.7
0.507ValTrp: 0.507 ± 0.143
3.483ValTyr: 3.483 ± 0.607
0.0ValXaa: 0.0 ± 0.0
Trp
0.443TrpAla: 0.443 ± 0.168
0.063TrpCys: 0.063 ± 0.07
0.823TrpAsp: 0.823 ± 0.193
0.95TrpGlu: 0.95 ± 0.261
0.317TrpPhe: 0.317 ± 0.139
0.823TrpGly: 0.823 ± 0.219
0.443TrpHis: 0.443 ± 0.175
1.14TrpIle: 1.14 ± 0.228
1.203TrpLys: 1.203 ± 0.203
1.393TrpLeu: 1.393 ± 0.32
0.507TrpMet: 0.507 ± 0.155
1.393TrpAsn: 1.393 ± 0.502
0.0TrpPro: 0.0 ± 0.0
0.697TrpGln: 0.697 ± 0.186
0.57TrpArg: 0.57 ± 0.146
0.95TrpSer: 0.95 ± 0.335
0.697TrpThr: 0.697 ± 0.189
0.76TrpVal: 0.76 ± 0.183
0.317TrpTrp: 0.317 ± 0.141
0.443TrpTyr: 0.443 ± 0.149
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.217TyrAla: 2.217 ± 0.421
0.443TyrCys: 0.443 ± 0.195
2.787TyrAsp: 2.787 ± 0.423
3.673TyrGlu: 3.673 ± 0.511
2.217TyrPhe: 2.217 ± 0.339
2.66TyrGly: 2.66 ± 0.391
0.76TyrHis: 0.76 ± 0.214
2.533TyrIle: 2.533 ± 0.457
2.153TyrLys: 2.153 ± 0.397
2.85TyrLeu: 2.85 ± 0.414
1.647TyrMet: 1.647 ± 0.327
2.153TyrAsn: 2.153 ± 0.377
0.823TyrPro: 0.823 ± 0.208
1.52TyrGln: 1.52 ± 0.269
1.773TyrArg: 1.773 ± 0.305
2.66TyrSer: 2.66 ± 0.439
2.66TyrThr: 2.66 ± 0.445
2.027TyrVal: 2.027 ± 0.364
0.19TyrTrp: 0.19 ± 0.109
1.71TyrTyr: 1.71 ± 0.362
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (15790 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski