Veillonella criceti
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1926 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A380NKL7|A0A380NKL7_9FIRM Uncharacterized protein OS=Veillonella criceti OX=103891 GN=NCTC12020_00733 PE=4 SV=1
MM1 pKa = 7.44 QLKK4 pKa = 9.25 QWLRR8 pKa = 11.84 PFILGFTLQLFIVAVPHH25 pKa = 5.5 TAFANWLSFGDD36 pKa = 4.13 MYY38 pKa = 11.48 AGATSEE44 pKa = 4.35 GLILSDD50 pKa = 5.32 SLLSLNGQTVTMQGYY65 pKa = 7.43 MAPPLKK71 pKa = 10.34 PSINFFVLTEE81 pKa = 4.25 TPMSICPFCSTDD93 pKa = 3.78 ADD95 pKa = 3.83 WPSDD99 pKa = 3.33 IVVVYY104 pKa = 10.86 LDD106 pKa = 4.21 DD107 pKa = 4.8 AVTALPYY114 pKa = 10.82 DD115 pKa = 3.36 QDD117 pKa = 3.44 ITVTGQLDD125 pKa = 3.35 VGSYY129 pKa = 11.08 VDD131 pKa = 4.13 GEE133 pKa = 4.57 TGFVSLVRR141 pKa = 11.84 LLGSEE146 pKa = 4.76 VYY148 pKa = 10.52
Molecular weight: 16.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.801
IPC2_protein 3.745
IPC_protein 3.706
Toseland 3.49
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.706
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.656
Nozaki 3.859
DTASelect 4.113
Thurlkill 3.567
EMBOSS 3.706
Sillero 3.834
Patrickios 0.299
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.768
Protein with the highest isoelectric point:
>tr|A0A380NM51|A0A380NM51_9FIRM Response regulator ArlR OS=Veillonella criceti OX=103891 GN=arlR PE=4 SV=1
MM1 pKa = 6.79 YY2 pKa = 9.92 RR3 pKa = 11.84 KK4 pKa = 10.09 AILDD8 pKa = 3.79 SQTSIHH14 pKa = 6.52 APRR17 pKa = 11.84 MRR19 pKa = 11.84 SDD21 pKa = 3.6 CNRR24 pKa = 11.84 ARR26 pKa = 11.84 RR27 pKa = 11.84 LTSQGDD33 pKa = 3.6 FNPRR37 pKa = 11.84 SPNEE41 pKa = 3.76 EE42 pKa = 3.68 RR43 pKa = 11.84 QGHH46 pKa = 5.05 HH47 pKa = 6.52 RR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 APRR53 pKa = 11.84 GDD55 pKa = 3.59 TSIHH59 pKa = 5.83 APRR62 pKa = 11.84 MRR64 pKa = 11.84 SDD66 pKa = 3.47 KK67 pKa = 10.96 EE68 pKa = 3.97 EE69 pKa = 4.46 KK70 pKa = 10.3 EE71 pKa = 4.02 LLKK74 pKa = 11.03 DD75 pKa = 4.45 FINFNPRR82 pKa = 11.84 SPNEE86 pKa = 3.59 EE87 pKa = 3.79 RR88 pKa = 11.84 LEE90 pKa = 4.02 KK91 pKa = 10.36 FLNDD95 pKa = 3.57 GEE97 pKa = 4.71 VTFTSIHH104 pKa = 5.95 APRR107 pKa = 11.84 MRR109 pKa = 11.84 SDD111 pKa = 3.4 NPPIIRR117 pKa = 11.84 ICTHH121 pKa = 6.55 KK122 pKa = 10.63 YY123 pKa = 9.66 FNPRR127 pKa = 11.84 SPNEE131 pKa = 3.68 EE132 pKa = 3.55 RR133 pKa = 11.84 RR134 pKa = 11.84 PRR136 pKa = 11.84 LPHH139 pKa = 4.75 EE140 pKa = 4.3 HH141 pKa = 6.78 RR142 pKa = 4.84
Molecular weight: 16.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.443
IPC_protein 10.379
Toseland 10.73
ProMoST 10.833
Dawson 10.789
Bjellqvist 10.555
Wikipedia 11.038
Rodwell 10.833
Grimsley 10.833
Solomon 10.994
Lehninger 10.95
Nozaki 10.716
DTASelect 10.54
Thurlkill 10.716
EMBOSS 11.155
Sillero 10.73
Patrickios 10.584
IPC_peptide 10.994
IPC2_peptide 9.692
IPC2.peptide.svr19 8.901
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1926
0
1926
638175
37
6487
331.3
36.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.693 ± 0.067
0.969 ± 0.024
5.329 ± 0.06
6.511 ± 0.068
3.758 ± 0.052
7.753 ± 0.101
1.988 ± 0.029
7.132 ± 0.057
5.982 ± 0.044
9.362 ± 0.08
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.784 ± 0.041
4.284 ± 0.071
3.671 ± 0.035
3.595 ± 0.037
4.292 ± 0.059
5.492 ± 0.044
6.247 ± 0.08
7.722 ± 0.056
0.959 ± 0.02
3.476 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here