Lepus americanus faeces associated genomovirus SHP7

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; unclassified Genomoviridae

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5CL77|A0A2Z5CL77_9VIRU Capsid protein OS=Lepus americanus faeces associated genomovirus SHP7 OX=2219120 PE=4 SV=1
MM1 pKa = 7.73PFVCNARR8 pKa = 11.84YY9 pKa = 8.99FLVTYY14 pKa = 9.3SHH16 pKa = 7.29VEE18 pKa = 3.84EE19 pKa = 5.21LDD21 pKa = 3.32PFALVNFFGSLGAEE35 pKa = 4.22CIVGLEE41 pKa = 4.54PYY43 pKa = 10.16NATLGVHH50 pKa = 5.51FHH52 pKa = 6.04VFADD56 pKa = 4.64FGRR59 pKa = 11.84KK60 pKa = 8.16FRR62 pKa = 11.84SRR64 pKa = 11.84RR65 pKa = 11.84TDD67 pKa = 2.68IFDD70 pKa = 3.27VDD72 pKa = 3.91GFHH75 pKa = 7.48PNISPSRR82 pKa = 11.84GTPEE86 pKa = 3.98AGYY89 pKa = 10.67DD90 pKa = 3.63YY91 pKa = 10.72AIKK94 pKa = 10.92NGDD97 pKa = 3.51VVAGGLARR105 pKa = 11.84PSRR108 pKa = 11.84VGDD111 pKa = 3.35SGRR114 pKa = 11.84AAKK117 pKa = 8.64WHH119 pKa = 6.19QILDD123 pKa = 3.47AEE125 pKa = 4.47TRR127 pKa = 11.84DD128 pKa = 3.66EE129 pKa = 5.25FFALCEE135 pKa = 3.97EE136 pKa = 5.06LDD138 pKa = 3.88PEE140 pKa = 4.71RR141 pKa = 11.84LVCSFGQIQKK151 pKa = 9.89FADD154 pKa = 2.8WRR156 pKa = 11.84FAVEE160 pKa = 4.24PKK162 pKa = 10.32PYY164 pKa = 10.41ANPDD168 pKa = 3.33GVFDD172 pKa = 4.41FADD175 pKa = 3.77HH176 pKa = 7.25GDD178 pKa = 3.94LEE180 pKa = 4.68VSKK183 pKa = 11.04SLILWGPSRR192 pKa = 11.84MGKK195 pKa = 6.86TVWARR200 pKa = 11.84SLGNHH205 pKa = 7.1LYY207 pKa = 10.71FGGIFSARR215 pKa = 11.84DD216 pKa = 2.97IGRR219 pKa = 11.84AGVEE223 pKa = 3.85YY224 pKa = 10.77AVFDD228 pKa = 5.39DD229 pKa = 3.46IAGGIKK235 pKa = 10.03FFPRR239 pKa = 11.84FKK241 pKa = 10.79DD242 pKa = 3.12WLGCQMEE249 pKa = 4.36FMVKK253 pKa = 9.32EE254 pKa = 4.5MYY256 pKa = 10.15RR257 pKa = 11.84DD258 pKa = 3.31PHH260 pKa = 6.37LFRR263 pKa = 11.84WGRR266 pKa = 11.84PAIWIANSDD275 pKa = 3.56PRR277 pKa = 11.84HH278 pKa = 6.41DD279 pKa = 3.93MTHH282 pKa = 6.58EE283 pKa = 4.84DD284 pKa = 4.16IVWLEE289 pKa = 4.01ANCMFVEE296 pKa = 4.52ISSAIFHH303 pKa = 6.76ANIEE307 pKa = 4.2

Molecular weight: 34.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5CL77|A0A2Z5CL77_9VIRU Capsid protein OS=Lepus americanus faeces associated genomovirus SHP7 OX=2219120 PE=4 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84SRR5 pKa = 11.84YY6 pKa = 8.86YY7 pKa = 10.7GGRR10 pKa = 11.84KK11 pKa = 4.59TTGWANSKK19 pKa = 9.58RR20 pKa = 11.84RR21 pKa = 11.84KK22 pKa = 9.36GRR24 pKa = 11.84SKK26 pKa = 10.76RR27 pKa = 11.84RR28 pKa = 11.84VSYY31 pKa = 10.58AKK33 pKa = 9.91KK34 pKa = 8.41RR35 pKa = 11.84TYY37 pKa = 10.46RR38 pKa = 11.84KK39 pKa = 9.01PRR41 pKa = 11.84MSNRR45 pKa = 11.84RR46 pKa = 11.84ILNVTSIKK54 pKa = 10.58KK55 pKa = 9.75RR56 pKa = 11.84DD57 pKa = 3.59TMMCWSNSTSASQKK71 pKa = 10.55GSTTYY76 pKa = 7.6TTNAAIVNGSTPDD89 pKa = 3.47SDD91 pKa = 3.59TQTFLWNATARR102 pKa = 11.84DD103 pKa = 3.59NTIAFGGALGNKK115 pKa = 8.36FNPSTRR121 pKa = 11.84TSTSCYY127 pKa = 9.23MVGLAEE133 pKa = 5.39KK134 pKa = 10.48IEE136 pKa = 4.2IQCNTGMPWQWRR148 pKa = 11.84RR149 pKa = 11.84ICFTMKK155 pKa = 10.4GSQLVPNSSASGTAFSNAIEE175 pKa = 4.13TSAGYY180 pKa = 9.74VRR182 pKa = 11.84VMNQVLGNAGQDD194 pKa = 3.48PQYY197 pKa = 11.59SLMGVLFKK205 pKa = 11.27GQVGTDD211 pKa = 3.35WIDD214 pKa = 3.48PMTAPTDD221 pKa = 3.47NSRR224 pKa = 11.84ITIKK228 pKa = 10.35YY229 pKa = 9.61DD230 pKa = 3.27KK231 pKa = 10.6VVSLASGNEE240 pKa = 3.8DD241 pKa = 3.51GFIRR245 pKa = 11.84TYY247 pKa = 10.94KK248 pKa = 9.53RR249 pKa = 11.84WHH251 pKa = 6.23PMKK254 pKa = 10.21STLVYY259 pKa = 10.9DD260 pKa = 4.32DD261 pKa = 5.51DD262 pKa = 4.27EE263 pKa = 6.51LGGGEE268 pKa = 4.46KK269 pKa = 9.48ATSYY273 pKa = 9.62STLGKK278 pKa = 10.45AGMGDD283 pKa = 4.35YY284 pKa = 11.18YY285 pKa = 11.41VMDD288 pKa = 4.25MFRR291 pKa = 11.84SRR293 pKa = 11.84QGAATTDD300 pKa = 3.3QLTIRR305 pKa = 11.84PEE307 pKa = 3.67ATLYY311 pKa = 9.2WHH313 pKa = 7.08EE314 pKa = 4.26KK315 pKa = 9.3

Molecular weight: 35.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

622

307

315

311.0

35.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.039 ± 0.538

1.608 ± 0.247

6.431 ± 0.987

4.341 ± 1.315

5.788 ± 2.371

8.842 ± 0.034

2.09 ± 1.062

4.823 ± 0.508

4.984 ± 1.229

5.305 ± 0.629

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.376 ± 0.78

4.341 ± 0.771

4.18 ± 0.734

2.412 ± 0.789

7.395 ± 0.395

7.395 ± 1.554

6.592 ± 3.068

5.627 ± 0.863

2.572 ± 0.024

3.859 ± 0.66
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski