Sulfurirhabdus autotrophica
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3698 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R3Y5J1|A0A4R3Y5J1_9PROT Peptidyl-prolyl cis-trans isomerase C OS=Sulfurirhabdus autotrophica OX=1706046 GN=EDC63_10672 PE=4 SV=1
MM1 pKa = 7.36 YY2 pKa = 10.38 NQNCLLAAIDD12 pKa = 5.37 VVLTWDD18 pKa = 4.18 LPDD21 pKa = 3.57 EE22 pKa = 4.68 AFTDD26 pKa = 3.86 AVNDD30 pKa = 3.73 QVRR33 pKa = 11.84 LMAAIDD39 pKa = 5.08 HH40 pKa = 6.6 EE41 pKa = 4.79 DD42 pKa = 2.73 TWEE45 pKa = 3.83 FHH47 pKa = 6.6 TDD49 pKa = 3.59 FSS51 pKa = 4.61
Molecular weight: 5.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.775
IPC2_protein 3.668
IPC_protein 3.592
Toseland 3.389
ProMoST 3.808
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.617
Rodwell 3.439
Grimsley 3.312
Solomon 3.579
Lehninger 3.541
Nozaki 3.77
DTASelect 3.999
Thurlkill 3.49
EMBOSS 3.617
Sillero 3.732
Patrickios 1.863
IPC_peptide 3.567
IPC2_peptide 3.694
IPC2.peptide.svr19 3.714
Protein with the highest isoelectric point:
>tr|A0A4R3XRS3|A0A4R3XRS3_9PROT Putative alpha-E superfamily protein OS=Sulfurirhabdus autotrophica OX=1706046 GN=EDC63_13310 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.0 RR14 pKa = 11.84 THH16 pKa = 5.76 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 8.68 TPGGRR28 pKa = 11.84 GVIRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.08 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LAVV44 pKa = 3.42
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3698
0
3698
1141177
26
3343
308.6
34.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.317 ± 0.051
0.958 ± 0.019
5.129 ± 0.032
6.009 ± 0.046
4.002 ± 0.025
7.283 ± 0.041
2.339 ± 0.023
6.183 ± 0.036
5.152 ± 0.036
10.52 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.687 ± 0.021
3.979 ± 0.034
4.459 ± 0.026
4.142 ± 0.028
5.191 ± 0.034
6.274 ± 0.037
5.368 ± 0.042
6.948 ± 0.038
1.281 ± 0.015
2.779 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here