Kyrpidia tusciae (strain DSM 2912 / NBRC 15312 / T2) (Bacillus tusciae)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Alicyclobacillaceae; Kyrpidia; Kyrpidia tusciae

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3135 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5WPY9|D5WPY9_KYRT2 Polynucleotide adenylyltransferase region OS=Kyrpidia tusciae (strain DSM 2912 / NBRC 15312 / T2) OX=562970 GN=Btus_1696 PE=3 SV=1
MM1 pKa = 7.52AFVITSPCIDD11 pKa = 3.56EE12 pKa = 4.63KK13 pKa = 11.12AAEE16 pKa = 4.27CVEE19 pKa = 4.29VCPVDD24 pKa = 5.2AIHH27 pKa = 6.78EE28 pKa = 4.73GEE30 pKa = 4.16DD31 pKa = 3.39QYY33 pKa = 11.98YY34 pKa = 9.9IDD36 pKa = 5.97PDD38 pKa = 3.82TCIDD42 pKa = 3.96CGACEE47 pKa = 4.06PVCPVSAIYY56 pKa = 10.44QEE58 pKa = 5.35DD59 pKa = 4.38FVPDD63 pKa = 3.77DD64 pKa = 3.54QKK66 pKa = 11.83EE67 pKa = 4.33FIQKK71 pKa = 9.51NRR73 pKa = 11.84DD74 pKa = 3.38FFKK77 pKa = 10.87KK78 pKa = 10.44

Molecular weight: 8.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5WQQ5|D5WQQ5_KYRT2 TVP38/TMEM64 family membrane protein OS=Kyrpidia tusciae (strain DSM 2912 / NBRC 15312 / T2) OX=562970 GN=Btus_1969 PE=3 SV=1
MM1 pKa = 7.4RR2 pKa = 11.84TLSTVLGIIGGIMTVRR18 pKa = 11.84SALLRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84QSPAQAWIGRR35 pKa = 11.84LRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84IMM41 pKa = 3.57

Molecular weight: 4.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3135

0

3135

950009

30

2077

303.0

33.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.911 ± 0.048

0.806 ± 0.015

5.106 ± 0.034

6.841 ± 0.049

3.496 ± 0.027

8.804 ± 0.047

2.194 ± 0.02

5.018 ± 0.029

3.468 ± 0.031

10.056 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.47 ± 0.017

2.365 ± 0.025

5.219 ± 0.036

3.505 ± 0.029

7.944 ± 0.053

4.972 ± 0.03

4.846 ± 0.028

8.661 ± 0.036

1.644 ± 0.024

2.674 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski