Clostridium nexile CAG:348

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2321 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6QLP2|R6QLP2_9CLOT 4-alpha-glucanotransferase OS=Clostridium nexile CAG:348 OX=1263069 GN=BN618_00662 PE=3 SV=1
MM1 pKa = 7.76GFDD4 pKa = 3.69CNKK7 pKa = 9.06WALEE11 pKa = 3.85EE12 pKa = 5.08LLAQNWNYY20 pKa = 11.12DD21 pKa = 3.67GQCNPFQASYY31 pKa = 10.4IDD33 pKa = 4.21EE34 pKa = 4.73VIQKK38 pKa = 10.22LEE40 pKa = 4.02AGEE43 pKa = 4.27EE44 pKa = 4.18IEE46 pKa = 4.43EE47 pKa = 4.15KK48 pKa = 10.17TIIMDD53 pKa = 3.69EE54 pKa = 4.23KK55 pKa = 11.09GFDD58 pKa = 3.42ATTITQDD65 pKa = 4.86DD66 pKa = 3.74VDD68 pKa = 3.66QYY70 pKa = 11.94GII72 pKa = 3.78

Molecular weight: 8.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6PVD2|R6PVD2_9CLOT Uncharacterized protein OS=Clostridium nexile CAG:348 OX=1263069 GN=BN618_02266 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2321

0

2321

753615

31

2606

324.7

36.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.11 ± 0.055

1.343 ± 0.022

5.404 ± 0.041

8.415 ± 0.059

4.052 ± 0.035

6.961 ± 0.046

1.706 ± 0.02

7.581 ± 0.059

7.655 ± 0.049

8.786 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.101 ± 0.03

4.373 ± 0.036

3.014 ± 0.024

3.351 ± 0.031

3.981 ± 0.04

5.467 ± 0.038

5.482 ± 0.045

7.233 ± 0.044

0.863 ± 0.018

4.123 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski