Shewanella sp. phage 1/4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 235 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088C314|A0A088C314_9CAUD Uncharacterized protein OS=Shewanella sp. phage 1/4 OX=1458859 GN=S14_56 PE=4 SV=1
MM1 pKa = 7.61NIKK4 pKa = 10.2DD5 pKa = 4.26LQTLDD10 pKa = 3.9EE11 pKa = 4.14VLKK14 pKa = 10.62YY15 pKa = 10.73GSLGEE20 pKa = 4.25VVISTPEE27 pKa = 3.35DD28 pKa = 2.83WFYY31 pKa = 11.66LDD33 pKa = 5.29KK34 pKa = 11.07IVDD37 pKa = 3.56MEE39 pKa = 4.77LNNKK43 pKa = 7.29YY44 pKa = 8.21TLGFDD49 pKa = 3.6AGQEE53 pKa = 3.99EE54 pKa = 4.87SSIEE58 pKa = 3.87SEE60 pKa = 4.53YY61 pKa = 10.99EE62 pKa = 3.87FEE64 pKa = 4.9CGYY67 pKa = 10.48EE68 pKa = 3.65QGLRR72 pKa = 11.84QGGVII77 pKa = 4.14

Molecular weight: 8.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088C327|A0A088C327_9CAUD Uncharacterized protein OS=Shewanella sp. phage 1/4 OX=1458859 GN=S14_63 PE=4 SV=1
MM1 pKa = 7.12VRR3 pKa = 11.84SLNGKK8 pKa = 7.47QCWLAQITSVAAKK21 pKa = 10.47GFVYY25 pKa = 10.35WSRR28 pKa = 11.84LKK30 pKa = 10.78GGRR33 pKa = 11.84IQHH36 pKa = 5.98LTNNTSDD43 pKa = 3.35VSKK46 pKa = 11.05LKK48 pKa = 10.01TYY50 pKa = 11.12SKK52 pKa = 11.41

Molecular weight: 5.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

235

0

235

38953

22

765

165.8

18.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.381 ± 0.21

1.569 ± 0.085

6.549 ± 0.128

6.364 ± 0.176

3.936 ± 0.104

6.626 ± 0.173

2.041 ± 0.083

6.469 ± 0.158

7.152 ± 0.298

8.603 ± 0.171

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.834 ± 0.111

5.679 ± 0.145

3.029 ± 0.139

3.227 ± 0.108

3.912 ± 0.119

6.898 ± 0.163

6.798 ± 0.241

7.114 ± 0.141

1.592 ± 0.085

4.228 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski