Cnuella takakiae
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4753 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M5BP89|A0A1M5BP89_9BACT Membrane-associated protein OS=Cnuella takakiae OX=1302690 GN=SAMN05444008_10833 PE=3 SV=1
MM1 pKa = 7.44 NFIDD5 pKa = 5.76 FEE7 pKa = 4.51 KK8 pKa = 10.83 KK9 pKa = 9.98 LNQRR13 pKa = 11.84 AAQLSYY19 pKa = 10.83 EE20 pKa = 4.08 EE21 pKa = 5.63 RR22 pKa = 11.84 IAQGTNICKK31 pKa = 10.43 GLFPYY36 pKa = 10.61 YY37 pKa = 10.54 KK38 pKa = 9.81 EE39 pKa = 4.16 FANEE43 pKa = 3.72 ASFGNPDD50 pKa = 3.35 VLLDD54 pKa = 3.61 SIRR57 pKa = 11.84 FVEE60 pKa = 4.98 SGEE63 pKa = 3.86 QDD65 pKa = 2.52 VDD67 pKa = 3.72 QIYY70 pKa = 10.67 EE71 pKa = 3.95 FLDD74 pKa = 3.54 NLEE77 pKa = 4.31 EE78 pKa = 4.2 VCPDD82 pKa = 3.08 AEE84 pKa = 4.26 EE85 pKa = 4.55 YY86 pKa = 11.02 EE87 pKa = 4.27 EE88 pKa = 6.1 GEE90 pKa = 4.45 YY91 pKa = 11.25 ALNACGAVNALLLQVAEE108 pKa = 4.29 PDD110 pKa = 3.68 EE111 pKa = 4.78 PEE113 pKa = 4.35 HH114 pKa = 6.22 FVEE117 pKa = 4.95 VALSYY122 pKa = 11.43 YY123 pKa = 8.12 EE124 pKa = 4.33 TIEE127 pKa = 4.45 ATIQDD132 pKa = 4.15 DD133 pKa = 4.19 AEE135 pKa = 4.21 EE136 pKa = 4.48 DD137 pKa = 3.52 MSDD140 pKa = 4.28 EE141 pKa = 4.23 EE142 pKa = 5.91 LEE144 pKa = 4.14 MHH146 pKa = 6.94 PMLAEE151 pKa = 3.83 ARR153 pKa = 11.84 RR154 pKa = 11.84 FLLASS159 pKa = 3.71
Molecular weight: 18.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.872
IPC_protein 3.821
Toseland 3.643
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.63
Rodwell 3.656
Grimsley 3.554
Solomon 3.757
Lehninger 3.706
Nozaki 3.884
DTASelect 3.999
Thurlkill 3.668
EMBOSS 3.656
Sillero 3.923
Patrickios 1.062
IPC_peptide 3.757
IPC2_peptide 3.91
IPC2.peptide.svr19 3.817
Protein with the highest isoelectric point:
>tr|A0A1M4Z496|A0A1M4Z496_9BACT Transposase domain OS=Cnuella takakiae OX=1302690 GN=SAMN05444008_10580 PE=4 SV=1
MM1 pKa = 7.06 TASFLISYY9 pKa = 8.6 LEE11 pKa = 3.86 RR12 pKa = 11.84 AGWQQVRR19 pKa = 11.84 QQGIHH24 pKa = 7.06 CILSHH29 pKa = 6.79 PNHH32 pKa = 7.04 PNLLSIPDD40 pKa = 4.04 LGEE43 pKa = 3.8 QALKK47 pKa = 10.4 PSLVNDD53 pKa = 3.89 ICRR56 pKa = 11.84 EE57 pKa = 3.9 AGLKK61 pKa = 10.35 GRR63 pKa = 11.84 VHH65 pKa = 7.04 KK66 pKa = 10.87 VQFSPRR72 pKa = 11.84 GIVTMVRR79 pKa = 11.84 NVLRR83 pKa = 11.84 LSRR86 pKa = 3.97
Molecular weight: 9.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.458
IPC_protein 10.131
Toseland 10.613
ProMoST 10.833
Dawson 10.701
Bjellqvist 10.409
Wikipedia 10.891
Rodwell 10.877
Grimsley 10.73
Solomon 10.833
Lehninger 10.804
Nozaki 10.628
DTASelect 10.394
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.643
Patrickios 10.716
IPC_peptide 10.833
IPC2_peptide 9.663
IPC2.peptide.svr19 8.746
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4753
0
4753
1616750
24
3118
340.2
37.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.8 ± 0.037
0.832 ± 0.013
4.971 ± 0.023
5.513 ± 0.037
4.673 ± 0.022
7.468 ± 0.032
2.035 ± 0.018
5.807 ± 0.029
5.662 ± 0.034
9.861 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.357 ± 0.018
4.901 ± 0.033
4.309 ± 0.021
4.539 ± 0.024
4.913 ± 0.027
5.79 ± 0.026
5.736 ± 0.036
6.734 ± 0.025
1.33 ± 0.015
3.77 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here