Rattail cactus necrosis-associated virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Tobamovirus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9CU46|G9CU46_9VIRU Capsid protein OS=Rattail cactus necrosis-associated virus OX=1123754 GN=CP PE=3 SV=1
MM1 pKa = 7.94PYY3 pKa = 10.46INVQPKK9 pKa = 10.47DD10 pKa = 3.58FVYY13 pKa = 9.42LTRR16 pKa = 11.84SWVDD20 pKa = 3.28PQRR23 pKa = 11.84LIQFLSDD30 pKa = 3.18MQTQAFQAQQSRR42 pKa = 11.84TQLLNEE48 pKa = 4.59LSTMVVYY55 pKa = 10.75GPTKK59 pKa = 10.32SDD61 pKa = 3.31RR62 pKa = 11.84FPIDD66 pKa = 3.04TYY68 pKa = 11.11LINITKK74 pKa = 10.31GGLSAYY80 pKa = 9.15WFGLTQSADD89 pKa = 3.14TKK91 pKa = 11.25DD92 pKa = 3.25RR93 pKa = 11.84VFEE96 pKa = 4.11VSEE99 pKa = 3.87ARR101 pKa = 11.84AVTNAEE107 pKa = 4.32SKK109 pKa = 10.74LATQRR114 pKa = 11.84VDD116 pKa = 3.29DD117 pKa = 3.91ATVAIRR123 pKa = 11.84NAIKK127 pKa = 9.53STLSYY132 pKa = 10.63LIAGEE137 pKa = 4.72DD138 pKa = 3.28IYY140 pKa = 11.67SRR142 pKa = 11.84TSFEE146 pKa = 5.14AALGWIWQEE155 pKa = 3.94NLPPPPQTAASDD167 pKa = 3.58SRR169 pKa = 11.84TT170 pKa = 3.45

Molecular weight: 19.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9CU46|G9CU46_9VIRU Capsid protein OS=Rattail cactus necrosis-associated virus OX=1123754 GN=CP PE=3 SV=1
MM1 pKa = 7.82SSLCVSDD8 pKa = 3.95PAVLLSPEE16 pKa = 4.19LHH18 pKa = 6.63LKK20 pKa = 10.05VPQLKK25 pKa = 10.57SLVQFWKK32 pKa = 10.7KK33 pKa = 10.02PFKK36 pKa = 9.84TCSISMSDD44 pKa = 3.84VIKK47 pKa = 10.71VASSNPLSIAVDD59 pKa = 4.6FSSAMPSGPRR69 pKa = 11.84NFKK72 pKa = 10.0YY73 pKa = 10.74VYY75 pKa = 9.66ILAVVLTGRR84 pKa = 11.84WHH86 pKa = 7.06LSEE89 pKa = 4.59SCPGGATFVLYY100 pKa = 10.41DD101 pKa = 3.47KK102 pKa = 10.31RR103 pKa = 11.84LCGRR107 pKa = 11.84AEE109 pKa = 4.4GIYY112 pKa = 10.69GSFFSKK118 pKa = 10.41VSASKK123 pKa = 9.27FQVRR127 pKa = 11.84FSVGHH132 pKa = 5.88SMTVNDD138 pKa = 4.67LLRR141 pKa = 11.84NPLHH145 pKa = 6.66LCFSLEE151 pKa = 4.15SVPCQEE157 pKa = 4.5GYY159 pKa = 10.84EE160 pKa = 4.07PLSVEE165 pKa = 4.25VSSLQLFTDD174 pKa = 5.29CILEE178 pKa = 4.11EE179 pKa = 4.21SLSAKK184 pKa = 9.2LVKK187 pKa = 10.65YY188 pKa = 9.06PALCTDD194 pKa = 3.17NMSVISNVDD203 pKa = 3.21DD204 pKa = 4.61VIFKK208 pKa = 10.53FNSVLNVDD216 pKa = 3.91NVPNSVKK223 pKa = 10.74NVVKK227 pKa = 10.49CNKK230 pKa = 8.47VFKK233 pKa = 10.35GRR235 pKa = 11.84RR236 pKa = 11.84SRR238 pKa = 11.84GKK240 pKa = 8.77WVGSQNEE247 pKa = 4.35VVVKK251 pKa = 10.65DD252 pKa = 3.73GTNVTPHH259 pKa = 6.86RR260 pKa = 11.84LIAKK264 pKa = 8.7DD265 pKa = 3.66ALHH268 pKa = 6.07QRR270 pKa = 11.84TTEE273 pKa = 3.96RR274 pKa = 11.84LRR276 pKa = 11.84LPDD279 pKa = 3.38QVMGRR284 pKa = 11.84PPASDD289 pKa = 3.1TVSEE293 pKa = 4.97RR294 pKa = 11.84YY295 pKa = 9.64ADD297 pKa = 3.59SGFPSTAVSHH307 pKa = 5.25TTSEE311 pKa = 4.11RR312 pKa = 11.84TVDD315 pKa = 3.85DD316 pKa = 4.01GGLRR320 pKa = 11.84SDD322 pKa = 3.99QIGPFPYY329 pKa = 9.72RR330 pKa = 11.84YY331 pKa = 9.8VPYY334 pKa = 10.81

Molecular weight: 36.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3292

170

1642

823.0

92.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.652 ± 0.459

2.309 ± 0.34

6.713 ± 0.38

4.465 ± 0.157

5.437 ± 0.296

5.498 ± 0.357

2.552 ± 0.454

4.253 ± 0.33

5.65 ± 0.319

9.022 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.853 ± 0.072

4.587 ± 0.248

4.617 ± 0.39

3.645 ± 0.578

5.437 ± 0.083

7.442 ± 1.148

5.68 ± 0.542

9.721 ± 0.625

1.397 ± 0.142

3.038 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski