Deinococcus irradiatisoli
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2903 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z3JFY9|A0A2Z3JFY9_9DEIO Carboxylic ester hydrolase OS=Deinococcus irradiatisoli OX=2202254 GN=DKM44_13425 PE=3 SV=1
MM1 pKa = 6.96 NTPSEE6 pKa = 4.03 LRR8 pKa = 11.84 YY9 pKa = 10.14 APSHH13 pKa = 5.94 EE14 pKa = 4.17 WLSDD18 pKa = 3.35 DD19 pKa = 3.67 GTVGISDD26 pKa = 4.46 FAQDD30 pKa = 3.29 QLGDD34 pKa = 3.68 VVYY37 pKa = 11.21 VEE39 pKa = 4.37 LPEE42 pKa = 4.24 VGRR45 pKa = 11.84 EE46 pKa = 4.01 VQAGEE51 pKa = 4.07 TVAVVEE57 pKa = 4.56 SVKK60 pKa = 10.0 TASDD64 pKa = 3.45 IYY66 pKa = 11.02 APASGTITAVNEE78 pKa = 3.96 ALAGSPEE85 pKa = 4.46 LVNGDD90 pKa = 4.1 CYY92 pKa = 11.57 GEE94 pKa = 4.15 GWLFKK99 pKa = 10.85 LDD101 pKa = 3.56 VTAEE105 pKa = 4.1 SGDD108 pKa = 3.99 LLDD111 pKa = 4.11 AASYY115 pKa = 9.39 EE116 pKa = 4.35 AANSS120 pKa = 3.58
Molecular weight: 12.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.767
IPC2_protein 3.668
IPC_protein 3.605
Toseland 3.414
ProMoST 3.77
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.516
Rodwell 3.439
Grimsley 3.338
Solomon 3.567
Lehninger 3.528
Nozaki 3.719
DTASelect 3.884
Thurlkill 3.478
EMBOSS 3.528
Sillero 3.732
Patrickios 0.769
IPC_peptide 3.567
IPC2_peptide 3.706
IPC2.peptide.svr19 3.684
Protein with the highest isoelectric point:
>tr|A0A2Z3JEN9|A0A2Z3JEN9_9DEIO Uncharacterized protein OS=Deinococcus irradiatisoli OX=2202254 GN=DKM44_09505 PE=4 SV=1
MM1 pKa = 7.51 SAPHH5 pKa = 6.7 LADD8 pKa = 3.14 ATLRR12 pKa = 11.84 AYY14 pKa = 10.48 ALGDD18 pKa = 3.76 LPLPAQQAAEE28 pKa = 4.15 GHH30 pKa = 5.82 VLGCPLCRR38 pKa = 11.84 AQVIRR43 pKa = 11.84 WRR45 pKa = 11.84 DD46 pKa = 3.3 DD47 pKa = 3.04 FAAQVDD53 pKa = 4.2 ALPSAGRR60 pKa = 11.84 LPPLRR65 pKa = 11.84 RR66 pKa = 11.84 PHH68 pKa = 6.21 GAPAKK73 pKa = 9.95 RR74 pKa = 11.84 HH75 pKa = 5.43 LPPRR79 pKa = 11.84 WSALTLPGLLMLSVLALGWGGWQQHH104 pKa = 4.94 QASALRR110 pKa = 11.84 AEE112 pKa = 4.39 QRR114 pKa = 11.84 QVAGWLAQPQLKK126 pKa = 9.38 VLAMQDD132 pKa = 3.25 TKK134 pKa = 10.92 RR135 pKa = 11.84 RR136 pKa = 11.84 PTGQLLLLPSRR147 pKa = 11.84 EE148 pKa = 4.09 VLFVLPPPAPGKK160 pKa = 10.52 VYY162 pKa = 9.6 QVWVAAHH169 pKa = 5.47 WHH171 pKa = 6.47 RR172 pKa = 11.84 GDD174 pKa = 3.73 KK175 pKa = 9.21 LTPQLSSRR183 pKa = 11.84 RR184 pKa = 11.84 GTFSASVGEE193 pKa = 3.91 NDD195 pKa = 3.9 YY196 pKa = 11.56 VCVSLEE202 pKa = 3.97 DD203 pKa = 4.18 AGRR206 pKa = 11.84 QPTKK210 pKa = 9.95 PSRR213 pKa = 11.84 ILGWSMVV220 pKa = 3.33
Molecular weight: 24.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.516
IPC_protein 10.365
Toseland 10.613
ProMoST 10.335
Dawson 10.701
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 10.833
Grimsley 10.76
Solomon 10.833
Lehninger 10.804
Nozaki 10.613
DTASelect 10.423
Thurlkill 10.613
EMBOSS 11.023
Sillero 10.643
Patrickios 10.57
IPC_peptide 10.847
IPC2_peptide 9.604
IPC2.peptide.svr19 8.617
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2903
0
2903
896936
33
4079
309.0
33.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.258 ± 0.065
0.555 ± 0.013
4.887 ± 0.033
5.633 ± 0.05
3.189 ± 0.027
8.982 ± 0.041
2.082 ± 0.023
3.61 ± 0.038
2.849 ± 0.044
12.047 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.84 ± 0.021
2.516 ± 0.031
5.697 ± 0.042
4.271 ± 0.039
6.941 ± 0.052
5.694 ± 0.039
5.495 ± 0.037
7.625 ± 0.031
1.389 ± 0.022
2.439 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here