Paenibacillus baekrokdamisoli

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6378 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G9ISX1|A0A3G9ISX1_9BACL Germination protein OS=Paenibacillus baekrokdamisoli OX=1712516 GN=Back11_04700 PE=3 SV=1
MM1 pKa = 7.68LDD3 pKa = 3.05EE4 pKa = 4.42TALLFQFSDD13 pKa = 3.68DD14 pKa = 3.65KK15 pKa = 11.57SAVLASQMLQEE26 pKa = 4.9LGYY29 pKa = 10.87EE30 pKa = 4.03PVIQQGQNVHH40 pKa = 5.17IHH42 pKa = 6.92LDD44 pKa = 3.77GSDD47 pKa = 3.34LTSALEE53 pKa = 3.99IAQSHH58 pKa = 6.14GGWLTTQSPITDD70 pKa = 3.16ACITDD75 pKa = 4.1DD76 pKa = 4.69AYY78 pKa = 11.29HH79 pKa = 7.01MDD81 pKa = 4.95TIPIPAHH88 pKa = 5.56VVNEE92 pKa = 4.0DD93 pKa = 4.44LIASEE98 pKa = 6.05DD99 pKa = 3.49IFDD102 pKa = 5.07PDD104 pKa = 3.82DD105 pKa = 4.11RR106 pKa = 11.84SYY108 pKa = 11.6GYY110 pKa = 10.7FSGDD114 pKa = 2.86VHH116 pKa = 5.92TT117 pKa = 5.42

Molecular weight: 12.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G9J5E2|A0A3G9J5E2_9BACL Beta-phosphoglucomutase-like phosphatase (HAD superfamily) OS=Paenibacillus baekrokdamisoli OX=1712516 GN=Back11_49300 PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84PTFRR6 pKa = 11.84PNVSKK11 pKa = 10.72RR12 pKa = 11.84KK13 pKa = 8.96KK14 pKa = 8.25VHH16 pKa = 5.48GFRR19 pKa = 11.84KK20 pKa = 10.03RR21 pKa = 11.84MASANGRR28 pKa = 11.84KK29 pKa = 9.15VLSARR34 pKa = 11.84RR35 pKa = 11.84QRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.84VLSAA44 pKa = 4.11

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6378

0

6378

2067881

39

3568

324.2

36.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.871 ± 0.045

0.753 ± 0.008

5.212 ± 0.022

6.423 ± 0.036

4.235 ± 0.027

7.211 ± 0.042

2.104 ± 0.015

7.133 ± 0.033

5.552 ± 0.029

9.938 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.811 ± 0.016

4.18 ± 0.025

3.924 ± 0.019

3.872 ± 0.022

4.716 ± 0.027

6.631 ± 0.028

5.595 ± 0.04

6.956 ± 0.031

1.358 ± 0.013

3.525 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski