Megasphaera sp. AM44-1BH

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Veillonellales; Veillonellaceae; Megasphaera; unclassified Megasphaera

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2578 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A413QPG3|A0A413QPG3_9FIRM Uncharacterized protein OS=Megasphaera sp. AM44-1BH OX=2292358 GN=DW949_13150 PE=4 SV=1
DDD2 pKa = 3.0ALAVGKKK9 pKa = 10.19AASYYY14 pKa = 10.71NAAGVEEE21 pKa = 4.34EEE23 pKa = 4.56LQSDDD28 pKa = 4.4LYYY31 pKa = 10.52EE32 pKa = 3.82CEEE35 pKa = 4.14ANSSDDD41 pKa = 3.05DDD43 pKa = 3.38FVSIHHH49 pKa = 6.01NAAEEE54 pKa = 3.99EEE56 pKa = 4.3EE57 pKa = 4.53NGTEEE62 pKa = 4.2WACTGSYYY70 pKa = 9.3GSMLANCIQSQLVDDD85 pKa = 3.53LGTTDDD91 pKa = 4.08GVKKK95 pKa = 9.55ATPGVNGLYYY105 pKa = 10.73LTNTDDD111 pKa = 3.12PAVLVEEE118 pKa = 4.36AFITNPSDDD127 pKa = 3.65EE128 pKa = 5.27EE129 pKa = 4.13LASAQDDD136 pKa = 3.59LARR139 pKa = 11.84AVARR143 pKa = 11.84GVTDDD148 pKa = 3.36YY149 pKa = 11.08EE150 pKa = 3.99LILGGK

Molecular weight: 16.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A413QZI8|A0A413QZI8_9FIRM Regulatory protein RecX OS=Megasphaera sp. AM44-1BH OX=2292358 GN=recX PE=3 SV=1
MM1 pKa = 7.39ISVRR5 pKa = 11.84QTRR8 pKa = 11.84DD9 pKa = 2.82LPRR12 pKa = 11.84PSSRR16 pKa = 11.84FHH18 pKa = 6.3LTMDD22 pKa = 4.09TLGSGYY28 pKa = 10.57GLPSTGRR35 pKa = 11.84PRR37 pKa = 11.84DD38 pKa = 3.7FHH40 pKa = 7.62PLEE43 pKa = 4.61CAHH46 pKa = 7.39AGRR49 pKa = 11.84TKK51 pKa = 10.71KK52 pKa = 10.01PVAILTVTGFLSVYY66 pKa = 10.37LL67 pKa = 4.09

Molecular weight: 7.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2578

0

2578

835493

27

2942

324.1

35.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.803 ± 0.051

1.431 ± 0.023

5.862 ± 0.048

6.023 ± 0.046

3.858 ± 0.038

7.621 ± 0.052

2.396 ± 0.022

6.886 ± 0.04

5.617 ± 0.04

9.15 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.043 ± 0.028

3.613 ± 0.049

4.003 ± 0.035

3.695 ± 0.029

5.01 ± 0.047

5.537 ± 0.036

5.683 ± 0.062

7.043 ± 0.044

1.023 ± 0.017

3.706 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski