Amino acid dipepetide frequency for Mycobacterium phage Cueylyss

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.826AlaAla: 12.826 ± 1.178
0.529AlaCys: 0.529 ± 0.16
6.545AlaAsp: 6.545 ± 0.683
6.083AlaGlu: 6.083 ± 0.808
3.107AlaPhe: 3.107 ± 0.504
8.331AlaGly: 8.331 ± 0.86
1.322AlaHis: 1.322 ± 0.336
4.298AlaIle: 4.298 ± 0.569
4.298AlaLys: 4.298 ± 0.605
8.926AlaLeu: 8.926 ± 0.989
2.05AlaMet: 2.05 ± 0.353
2.446AlaAsn: 2.446 ± 0.427
5.157AlaPro: 5.157 ± 0.693
3.438AlaGln: 3.438 ± 0.483
6.479AlaArg: 6.479 ± 0.609
4.628AlaSer: 4.628 ± 0.6
5.884AlaThr: 5.884 ± 0.544
8.661AlaVal: 8.661 ± 0.85
2.05AlaTrp: 2.05 ± 0.41
2.512AlaTyr: 2.512 ± 0.368
0.0AlaXaa: 0.0 ± 0.0
Cys
0.86CysAla: 0.86 ± 0.236
0.0CysCys: 0.0 ± 0.0
0.397CysAsp: 0.397 ± 0.147
0.463CysGlu: 0.463 ± 0.147
0.132CysPhe: 0.132 ± 0.091
0.463CysGly: 0.463 ± 0.195
0.132CysHis: 0.132 ± 0.086
0.264CysIle: 0.264 ± 0.128
0.198CysLys: 0.198 ± 0.118
0.264CysLeu: 0.264 ± 0.156
0.066CysMet: 0.066 ± 0.065
0.264CysAsn: 0.264 ± 0.111
0.264CysPro: 0.264 ± 0.128
0.331CysGln: 0.331 ± 0.134
0.331CysArg: 0.331 ± 0.13
0.331CysSer: 0.331 ± 0.138
0.198CysThr: 0.198 ± 0.112
0.331CysVal: 0.331 ± 0.129
0.198CysTrp: 0.198 ± 0.114
0.066CysTyr: 0.066 ± 0.055
0.0CysXaa: 0.0 ± 0.0
Asp
6.281AspAla: 6.281 ± 0.546
0.463AspCys: 0.463 ± 0.152
4.628AspAsp: 4.628 ± 0.458
3.57AspGlu: 3.57 ± 0.507
2.512AspPhe: 2.512 ± 0.392
6.347AspGly: 6.347 ± 0.683
1.124AspHis: 1.124 ± 0.276
2.512AspIle: 2.512 ± 0.431
2.512AspLys: 2.512 ± 0.476
6.942AspLeu: 6.942 ± 0.656
1.256AspMet: 1.256 ± 0.259
1.917AspAsn: 1.917 ± 0.357
5.025AspPro: 5.025 ± 0.538
1.521AspGln: 1.521 ± 0.327
3.901AspArg: 3.901 ± 0.476
3.107AspSer: 3.107 ± 0.55
4.364AspThr: 4.364 ± 0.54
5.223AspVal: 5.223 ± 0.621
1.983AspTrp: 1.983 ± 0.297
2.182AspTyr: 2.182 ± 0.361
0.0AspXaa: 0.0 ± 0.0
Glu
6.347GluAla: 6.347 ± 0.817
0.198GluCys: 0.198 ± 0.145
4.298GluAsp: 4.298 ± 0.531
5.091GluGlu: 5.091 ± 0.627
2.38GluPhe: 2.38 ± 0.486
4.364GluGly: 4.364 ± 0.492
1.256GluHis: 1.256 ± 0.311
3.702GluIle: 3.702 ± 0.456
2.777GluLys: 2.777 ± 0.454
6.215GluLeu: 6.215 ± 0.565
1.653GluMet: 1.653 ± 0.339
1.785GluAsn: 1.785 ± 0.319
2.314GluPro: 2.314 ± 0.423
3.041GluGln: 3.041 ± 0.479
3.438GluArg: 3.438 ± 0.489
3.504GluSer: 3.504 ± 0.368
3.438GluThr: 3.438 ± 0.547
5.223GluVal: 5.223 ± 0.561
1.653GluTrp: 1.653 ± 0.375
2.116GluTyr: 2.116 ± 0.449
0.0GluXaa: 0.0 ± 0.0
Phe
2.975PheAla: 2.975 ± 0.427
0.198PheCys: 0.198 ± 0.135
2.909PheAsp: 2.909 ± 0.341
2.248PheGlu: 2.248 ± 0.35
0.463PhePhe: 0.463 ± 0.192
3.636PheGly: 3.636 ± 0.529
0.793PheHis: 0.793 ± 0.273
1.256PheIle: 1.256 ± 0.25
1.455PheLys: 1.455 ± 0.256
2.446PheLeu: 2.446 ± 0.478
0.595PheMet: 0.595 ± 0.199
1.653PheAsn: 1.653 ± 0.35
1.587PhePro: 1.587 ± 0.336
1.124PheGln: 1.124 ± 0.249
1.983PheArg: 1.983 ± 0.361
1.917PheSer: 1.917 ± 0.375
2.116PheThr: 2.116 ± 0.362
1.587PheVal: 1.587 ± 0.383
0.595PheTrp: 0.595 ± 0.178
0.86PheTyr: 0.86 ± 0.266
0.0PheXaa: 0.0 ± 0.0
Gly
7.14GlyAla: 7.14 ± 0.964
0.661GlyCys: 0.661 ± 0.202
5.686GlyAsp: 5.686 ± 0.651
4.298GlyGlu: 4.298 ± 0.547
3.174GlyPhe: 3.174 ± 0.544
9.19GlyGly: 9.19 ± 2.343
1.851GlyHis: 1.851 ± 0.375
4.826GlyIle: 4.826 ± 0.71
3.57GlyLys: 3.57 ± 0.558
7.471GlyLeu: 7.471 ± 0.816
1.455GlyMet: 1.455 ± 0.326
3.24GlyAsn: 3.24 ± 0.376
3.769GlyPro: 3.769 ± 0.617
2.182GlyGln: 2.182 ± 0.361
4.76GlyArg: 4.76 ± 0.568
6.876GlySer: 6.876 ± 0.924
5.091GlyThr: 5.091 ± 0.688
6.083GlyVal: 6.083 ± 0.689
2.182GlyTrp: 2.182 ± 0.363
3.372GlyTyr: 3.372 ± 0.446
0.0GlyXaa: 0.0 ± 0.0
His
2.05HisAla: 2.05 ± 0.418
0.132HisCys: 0.132 ± 0.145
1.124HisAsp: 1.124 ± 0.253
1.653HisGlu: 1.653 ± 0.335
0.992HisPhe: 0.992 ± 0.259
1.388HisGly: 1.388 ± 0.391
0.727HisHis: 0.727 ± 0.254
0.727HisIle: 0.727 ± 0.203
1.19HisLys: 1.19 ± 0.307
1.455HisLeu: 1.455 ± 0.338
0.264HisMet: 0.264 ± 0.161
0.264HisAsn: 0.264 ± 0.115
1.256HisPro: 1.256 ± 0.252
1.124HisGln: 1.124 ± 0.294
1.124HisArg: 1.124 ± 0.225
0.595HisSer: 0.595 ± 0.225
1.256HisThr: 1.256 ± 0.278
1.521HisVal: 1.521 ± 0.345
0.661HisTrp: 0.661 ± 0.2
0.793HisTyr: 0.793 ± 0.249
0.0HisXaa: 0.0 ± 0.0
Ile
6.083IleAla: 6.083 ± 0.614
0.198IleCys: 0.198 ± 0.101
3.835IleAsp: 3.835 ± 0.507
3.57IleGlu: 3.57 ± 0.454
0.661IlePhe: 0.661 ± 0.182
4.231IleGly: 4.231 ± 0.515
0.992IleHis: 0.992 ± 0.28
2.248IleIle: 2.248 ± 0.324
2.182IleLys: 2.182 ± 0.326
3.372IleLeu: 3.372 ± 0.455
0.529IleMet: 0.529 ± 0.179
1.653IleAsn: 1.653 ± 0.368
3.107IlePro: 3.107 ± 0.414
1.653IleGln: 1.653 ± 0.341
3.636IleArg: 3.636 ± 0.476
3.24IleSer: 3.24 ± 0.537
3.57IleThr: 3.57 ± 0.353
2.777IleVal: 2.777 ± 0.557
0.529IleTrp: 0.529 ± 0.154
1.388IleTyr: 1.388 ± 0.249
0.0IleXaa: 0.0 ± 0.0
Lys
3.504LysAla: 3.504 ± 0.542
0.397LysCys: 0.397 ± 0.149
2.777LysAsp: 2.777 ± 0.508
1.917LysGlu: 1.917 ± 0.38
1.653LysPhe: 1.653 ± 0.344
2.645LysGly: 2.645 ± 0.45
0.926LysHis: 0.926 ± 0.272
2.843LysIle: 2.843 ± 0.516
2.05LysLys: 2.05 ± 0.525
3.174LysLeu: 3.174 ± 0.386
1.322LysMet: 1.322 ± 0.246
1.653LysAsn: 1.653 ± 0.33
2.711LysPro: 2.711 ± 0.437
1.785LysGln: 1.785 ± 0.38
2.645LysArg: 2.645 ± 0.41
2.579LysSer: 2.579 ± 0.44
2.512LysThr: 2.512 ± 0.421
3.107LysVal: 3.107 ± 0.505
0.661LysTrp: 0.661 ± 0.222
0.992LysTyr: 0.992 ± 0.253
0.0LysXaa: 0.0 ± 0.0
Leu
8.661LeuAla: 8.661 ± 0.901
0.397LeuCys: 0.397 ± 0.126
6.281LeuAsp: 6.281 ± 0.622
5.554LeuGlu: 5.554 ± 0.63
2.314LeuPhe: 2.314 ± 0.379
7.273LeuGly: 7.273 ± 0.77
2.05LeuHis: 2.05 ± 0.383
4.562LeuIle: 4.562 ± 0.538
3.702LeuLys: 3.702 ± 0.526
5.488LeuLeu: 5.488 ± 0.624
1.785LeuMet: 1.785 ± 0.305
2.711LeuAsn: 2.711 ± 0.413
5.554LeuPro: 5.554 ± 0.563
2.975LeuGln: 2.975 ± 0.51
5.554LeuArg: 5.554 ± 0.547
5.355LeuSer: 5.355 ± 0.547
6.017LeuThr: 6.017 ± 0.501
4.099LeuVal: 4.099 ± 0.557
1.322LeuTrp: 1.322 ± 0.377
2.512LeuTyr: 2.512 ± 0.415
0.0LeuXaa: 0.0 ± 0.0
Met
2.446MetAla: 2.446 ± 0.394
0.066MetCys: 0.066 ± 0.055
1.058MetAsp: 1.058 ± 0.225
1.388MetGlu: 1.388 ± 0.308
0.595MetPhe: 0.595 ± 0.169
1.521MetGly: 1.521 ± 0.265
0.264MetHis: 0.264 ± 0.122
0.793MetIle: 0.793 ± 0.257
1.058MetLys: 1.058 ± 0.25
0.992MetLeu: 0.992 ± 0.232
0.132MetMet: 0.132 ± 0.093
0.992MetAsn: 0.992 ± 0.184
1.19MetPro: 1.19 ± 0.233
0.397MetGln: 0.397 ± 0.147
1.124MetArg: 1.124 ± 0.282
2.116MetSer: 2.116 ± 0.364
1.587MetThr: 1.587 ± 0.254
1.124MetVal: 1.124 ± 0.32
0.264MetTrp: 0.264 ± 0.145
0.331MetTyr: 0.331 ± 0.147
0.0MetXaa: 0.0 ± 0.0
Asn
3.174AsnAla: 3.174 ± 0.515
0.331AsnCys: 0.331 ± 0.153
2.314AsnAsp: 2.314 ± 0.411
1.785AsnGlu: 1.785 ± 0.339
1.256AsnPhe: 1.256 ± 0.274
3.438AsnGly: 3.438 ± 0.504
0.727AsnHis: 0.727 ± 0.218
1.521AsnIle: 1.521 ± 0.34
0.661AsnLys: 0.661 ± 0.23
2.512AsnLeu: 2.512 ± 0.346
0.661AsnMet: 0.661 ± 0.22
0.793AsnAsn: 0.793 ± 0.18
2.579AsnPro: 2.579 ± 0.363
0.926AsnGln: 0.926 ± 0.244
1.322AsnArg: 1.322 ± 0.341
2.512AsnSer: 2.512 ± 0.446
1.851AsnThr: 1.851 ± 0.315
2.314AsnVal: 2.314 ± 0.447
0.793AsnTrp: 0.793 ± 0.207
1.256AsnTyr: 1.256 ± 0.293
0.0AsnXaa: 0.0 ± 0.0
Pro
5.025ProAla: 5.025 ± 0.521
0.264ProCys: 0.264 ± 0.119
4.496ProAsp: 4.496 ± 0.503
4.694ProGlu: 4.694 ± 0.583
2.116ProPhe: 2.116 ± 0.379
4.694ProGly: 4.694 ± 0.626
0.926ProHis: 0.926 ± 0.218
2.645ProIle: 2.645 ± 0.443
2.248ProLys: 2.248 ± 0.317
4.694ProLeu: 4.694 ± 0.556
0.727ProMet: 0.727 ± 0.212
1.719ProAsn: 1.719 ± 0.313
2.579ProPro: 2.579 ± 0.476
1.587ProGln: 1.587 ± 0.379
2.777ProArg: 2.777 ± 0.429
3.769ProSer: 3.769 ± 0.446
3.438ProThr: 3.438 ± 0.49
4.231ProVal: 4.231 ± 0.562
0.926ProTrp: 0.926 ± 0.276
1.653ProTyr: 1.653 ± 0.321
0.0ProXaa: 0.0 ± 0.0
Gln
3.174GlnAla: 3.174 ± 0.482
0.0GlnCys: 0.0 ± 0.0
1.19GlnAsp: 1.19 ± 0.353
1.653GlnGlu: 1.653 ± 0.301
1.256GlnPhe: 1.256 ± 0.225
2.645GlnGly: 2.645 ± 0.334
0.529GlnHis: 0.529 ± 0.164
2.909GlnIle: 2.909 ± 0.523
1.719GlnLys: 1.719 ± 0.404
3.769GlnLeu: 3.769 ± 0.592
0.793GlnMet: 0.793 ± 0.246
0.661GlnAsn: 0.661 ± 0.211
1.983GlnPro: 1.983 ± 0.367
1.785GlnGln: 1.785 ± 0.448
1.719GlnArg: 1.719 ± 0.391
1.719GlnSer: 1.719 ± 0.336
2.05GlnThr: 2.05 ± 0.345
2.512GlnVal: 2.512 ± 0.367
0.529GlnTrp: 0.529 ± 0.143
0.595GlnTyr: 0.595 ± 0.171
0.0GlnXaa: 0.0 ± 0.0
Arg
5.818ArgAla: 5.818 ± 0.594
0.463ArgCys: 0.463 ± 0.158
3.174ArgAsp: 3.174 ± 0.439
4.43ArgGlu: 4.43 ± 0.608
1.587ArgPhe: 1.587 ± 0.369
4.694ArgGly: 4.694 ± 0.657
0.992ArgHis: 0.992 ± 0.241
3.107ArgIle: 3.107 ± 0.44
2.711ArgLys: 2.711 ± 0.467
5.884ArgLeu: 5.884 ± 0.814
1.587ArgMet: 1.587 ± 0.349
2.314ArgAsn: 2.314 ± 0.413
2.711ArgPro: 2.711 ± 0.449
1.851ArgGln: 1.851 ± 0.365
5.025ArgArg: 5.025 ± 0.615
3.57ArgSer: 3.57 ± 0.496
3.24ArgThr: 3.24 ± 0.566
5.091ArgVal: 5.091 ± 0.546
1.124ArgTrp: 1.124 ± 0.26
1.851ArgTyr: 1.851 ± 0.301
0.0ArgXaa: 0.0 ± 0.0
Ser
6.347SerAla: 6.347 ± 0.947
0.397SerCys: 0.397 ± 0.172
3.702SerAsp: 3.702 ± 0.494
4.165SerGlu: 4.165 ± 0.482
1.917SerPhe: 1.917 ± 0.395
7.008SerGly: 7.008 ± 0.996
1.322SerHis: 1.322 ± 0.31
2.314SerIle: 2.314 ± 0.342
2.182SerLys: 2.182 ± 0.294
4.959SerLeu: 4.959 ± 0.627
1.455SerMet: 1.455 ± 0.286
2.314SerAsn: 2.314 ± 0.488
3.107SerPro: 3.107 ± 0.557
1.653SerGln: 1.653 ± 0.315
3.107SerArg: 3.107 ± 0.45
3.835SerSer: 3.835 ± 0.636
3.769SerThr: 3.769 ± 0.594
4.099SerVal: 4.099 ± 0.486
1.256SerTrp: 1.256 ± 0.288
1.19SerTyr: 1.19 ± 0.288
0.0SerXaa: 0.0 ± 0.0
Thr
6.281ThrAla: 6.281 ± 0.722
0.331ThrCys: 0.331 ± 0.169
4.364ThrAsp: 4.364 ± 0.563
4.43ThrGlu: 4.43 ± 0.594
2.182ThrPhe: 2.182 ± 0.386
6.545ThrGly: 6.545 ± 0.561
1.322ThrHis: 1.322 ± 0.326
2.579ThrIle: 2.579 ± 0.541
2.579ThrLys: 2.579 ± 0.352
5.818ThrLeu: 5.818 ± 0.653
0.926ThrMet: 0.926 ± 0.207
1.917ThrAsn: 1.917 ± 0.391
3.306ThrPro: 3.306 ± 0.52
1.983ThrGln: 1.983 ± 0.375
3.57ThrArg: 3.57 ± 0.532
3.306ThrSer: 3.306 ± 0.473
4.298ThrThr: 4.298 ± 0.638
5.355ThrVal: 5.355 ± 0.683
1.322ThrTrp: 1.322 ± 0.297
1.785ThrTyr: 1.785 ± 0.388
0.0ThrXaa: 0.0 ± 0.0
Val
6.942ValAla: 6.942 ± 0.651
0.331ValCys: 0.331 ± 0.143
5.554ValAsp: 5.554 ± 0.541
4.364ValGlu: 4.364 ± 0.538
2.645ValPhe: 2.645 ± 0.367
4.959ValGly: 4.959 ± 0.759
1.785ValHis: 1.785 ± 0.284
3.438ValIle: 3.438 ± 0.45
3.041ValLys: 3.041 ± 0.447
5.884ValLeu: 5.884 ± 0.528
0.992ValMet: 0.992 ± 0.276
2.711ValAsn: 2.711 ± 0.398
4.76ValPro: 4.76 ± 0.56
2.116ValGln: 2.116 ± 0.433
4.76ValArg: 4.76 ± 0.669
4.364ValSer: 4.364 ± 0.531
5.752ValThr: 5.752 ± 0.663
5.355ValVal: 5.355 ± 0.71
1.322ValTrp: 1.322 ± 0.265
2.05ValTyr: 2.05 ± 0.389
0.0ValXaa: 0.0 ± 0.0
Trp
1.124TrpAla: 1.124 ± 0.281
0.198TrpCys: 0.198 ± 0.109
1.851TrpAsp: 1.851 ± 0.326
1.19TrpGlu: 1.19 ± 0.253
0.793TrpPhe: 0.793 ± 0.193
1.455TrpGly: 1.455 ± 0.282
0.529TrpHis: 0.529 ± 0.16
1.521TrpIle: 1.521 ± 0.322
0.397TrpLys: 0.397 ± 0.196
1.455TrpLeu: 1.455 ± 0.308
0.595TrpMet: 0.595 ± 0.23
0.397TrpAsn: 0.397 ± 0.143
0.793TrpPro: 0.793 ± 0.231
0.727TrpGln: 0.727 ± 0.198
1.521TrpArg: 1.521 ± 0.338
1.322TrpSer: 1.322 ± 0.299
1.587TrpThr: 1.587 ± 0.305
1.917TrpVal: 1.917 ± 0.372
0.727TrpTrp: 0.727 ± 0.29
0.198TrpTyr: 0.198 ± 0.094
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.512TyrAla: 2.512 ± 0.368
0.0TyrCys: 0.0 ± 0.0
1.19TyrAsp: 1.19 ± 0.356
2.248TyrGlu: 2.248 ± 0.337
0.661TyrPhe: 0.661 ± 0.153
2.116TyrGly: 2.116 ± 0.412
0.727TyrHis: 0.727 ± 0.224
1.388TyrIle: 1.388 ± 0.259
1.322TyrLys: 1.322 ± 0.308
2.446TyrLeu: 2.446 ± 0.381
0.529TyrMet: 0.529 ± 0.181
1.322TyrAsn: 1.322 ± 0.298
1.455TyrPro: 1.455 ± 0.301
0.992TyrGln: 0.992 ± 0.269
2.446TyrArg: 2.446 ± 0.415
1.455TyrSer: 1.455 ± 0.253
2.182TyrThr: 2.182 ± 0.449
2.512TyrVal: 2.512 ± 0.349
0.198TyrTrp: 0.198 ± 0.121
0.463TyrTyr: 0.463 ± 0.175
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (15126 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski