Campylobacter virus CP220
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 194 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D5GV89|D5GV89_9CAUD Hypothetical phage protein OS=Campylobacter virus CP220 OX=722417 GN=CPT_0097 PE=4 SV=1
MM1 pKa = 6.82 YY2 pKa = 9.99 VARR5 pKa = 11.84 FNEE8 pKa = 4.17 YY9 pKa = 10.17 FGYY12 pKa = 10.27 DD13 pKa = 3.69 YY14 pKa = 11.5 QLMDD18 pKa = 3.94 VEE20 pKa = 4.74 TVDD23 pKa = 3.5 ILEE26 pKa = 4.93 NIYY29 pKa = 11.06 DD30 pKa = 3.83 SLGYY34 pKa = 9.2 MPRR37 pKa = 11.84 DD38 pKa = 3.63 PEE40 pKa = 5.28 PIDD43 pKa = 3.86 LEE45 pKa = 4.12 IVLEE49 pKa = 4.09 EE50 pKa = 4.85 FYY52 pKa = 10.45 NTDD55 pKa = 3.36 KK56 pKa = 11.01 IYY58 pKa = 10.82 VLCEE62 pKa = 3.82 ADD64 pKa = 3.57 DD65 pKa = 4.27 EE66 pKa = 4.81 EE67 pKa = 6.57 IEE69 pKa = 4.29 FCSCIDD75 pKa = 3.46 EE76 pKa = 4.43 VEE78 pKa = 4.07 PEE80 pKa = 4.11 IIIDD84 pKa = 3.69 FLKK87 pKa = 11.09 SKK89 pKa = 10.18 GHH91 pKa = 5.73 TFMNSNAA98 pKa = 3.77
Molecular weight: 11.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.864
IPC2_protein 3.694
IPC_protein 3.643
Toseland 3.452
ProMoST 3.808
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.541
Rodwell 3.478
Grimsley 3.363
Solomon 3.605
Lehninger 3.567
Nozaki 3.745
DTASelect 3.91
Thurlkill 3.503
EMBOSS 3.554
Sillero 3.77
Patrickios 0.146
IPC_peptide 3.605
IPC2_peptide 3.745
IPC2.peptide.svr19 3.696
Protein with the highest isoelectric point:
>tr|D5GV52|D5GV52_9CAUD Hypothetical phage protein OS=Campylobacter virus CP220 OX=722417 GN=CPT_0060 PE=4 SV=1
MM1 pKa = 8.25 DD2 pKa = 4.46 SLKK5 pKa = 10.73 LVKK8 pKa = 10.48 NLIKK12 pKa = 10.02 TKK14 pKa = 10.3 SVEE17 pKa = 3.74 KK18 pKa = 10.81 GQILKK23 pKa = 10.18 PGNLVIFKK31 pKa = 9.51 YY32 pKa = 10.38 NPKK35 pKa = 8.84 DD36 pKa = 3.31 TSVKK40 pKa = 9.92 YY41 pKa = 10.65 DD42 pKa = 3.6 RR43 pKa = 11.84 TPLCLVLRR51 pKa = 11.84 KK52 pKa = 9.82 SKK54 pKa = 10.75 SYY56 pKa = 8.62 TLGINFHH63 pKa = 6.52 WCPIPMRR70 pKa = 11.84 KK71 pKa = 8.28 MLLNAIFRR79 pKa = 11.84 LNKK82 pKa = 10.16 KK83 pKa = 9.73 NIKK86 pKa = 8.7 EE87 pKa = 4.02 NKK89 pKa = 9.04 PLDD92 pKa = 3.8 IDD94 pKa = 3.93 WYY96 pKa = 10.34 RR97 pKa = 11.84 IKK99 pKa = 10.97 PMLKK103 pKa = 9.73 KK104 pKa = 10.66 FGFFPIIRR112 pKa = 11.84 LYY114 pKa = 10.15 INSRR118 pKa = 11.84 IYY120 pKa = 10.45 RR121 pKa = 11.84 RR122 pKa = 11.84 AVKK125 pKa = 10.12 IPNEE129 pKa = 3.81 NMKK132 pKa = 10.57 QIIEE136 pKa = 4.46 TKK138 pKa = 8.35 TEE140 pKa = 3.87 TFIGVSAEE148 pKa = 3.98 VLYY151 pKa = 10.97 KK152 pKa = 10.55 KK153 pKa = 10.27 ALKK156 pKa = 10.1 DD157 pKa = 3.52 SKK159 pKa = 11.21 VSSKK163 pKa = 10.79 SKK165 pKa = 10.43 KK166 pKa = 9.75
Molecular weight: 19.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.164
IPC2_protein 9.663
IPC_protein 9.619
Toseland 10.57
ProMoST 10.072
Dawson 10.657
Bjellqvist 10.248
Wikipedia 10.76
Rodwell 11.374
Grimsley 10.687
Solomon 10.672
Lehninger 10.657
Nozaki 10.526
DTASelect 10.248
Thurlkill 10.54
EMBOSS 10.935
Sillero 10.57
Patrickios 11.082
IPC_peptide 10.687
IPC2_peptide 8.741
IPC2.peptide.svr19 8.632
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
194
0
194
52020
36
1386
268.1
30.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.419 ± 0.158
1.513 ± 0.124
6.867 ± 0.142
6.455 ± 0.167
5.165 ± 0.124
4.508 ± 0.152
1.376 ± 0.069
8.683 ± 0.153
9.585 ± 0.199
8.76 ± 0.164
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.744 ± 0.068
8.033 ± 0.142
2.82 ± 0.084
2.941 ± 0.092
3.147 ± 0.091
7.103 ± 0.176
5.702 ± 0.163
5.229 ± 0.134
0.702 ± 0.045
5.248 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here