Enterobacteria phage HK106
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K7P887|K7P887_9CAUD DNA helicase OS=Enterobacteria phage HK106 OX=432198 GN=HK106_052 PE=3 SV=1
MM1 pKa = 8.29 DD2 pKa = 4.56 YY3 pKa = 11.03 SQLSDD8 pKa = 3.38 FEE10 pKa = 4.82 INKK13 pKa = 9.58 RR14 pKa = 11.84 VAICCGFAPEE24 pKa = 4.42 DD25 pKa = 3.82 CEE27 pKa = 4.06 IAKK30 pKa = 10.45 LGTSIVGVEE39 pKa = 3.81 WDD41 pKa = 4.72 DD42 pKa = 3.5 EE43 pKa = 4.13 TGYY46 pKa = 11.09 AIKK49 pKa = 9.63 TVDD52 pKa = 3.32 YY53 pKa = 10.45 CKK55 pKa = 10.28 RR56 pKa = 11.84 PSDD59 pKa = 3.32 AWPIITEE66 pKa = 4.09 NNISIILDD74 pKa = 3.81 NPSMPCATDD83 pKa = 3.23 NARR86 pKa = 11.84 DD87 pKa = 3.79 LFDD90 pKa = 5.03 DD91 pKa = 4.5 AGPNVGVAYY100 pKa = 9.96 DD101 pKa = 3.76 NPLRR105 pKa = 11.84 AAMIVFLMIQDD116 pKa = 3.81 ANNAA120 pKa = 3.77
Molecular weight: 13.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.872
IPC_protein 3.846
Toseland 3.63
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.808
Rodwell 3.668
Grimsley 3.541
Solomon 3.834
Lehninger 3.795
Nozaki 3.973
DTASelect 4.228
Thurlkill 3.694
EMBOSS 3.821
Sillero 3.973
Patrickios 1.914
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.867
Protein with the highest isoelectric point:
>tr|A4KWV6|A4KWV6_9CAUD Uncharacterized protein OS=Enterobacteria phage HK106 OX=432198 GN=HK10603 PE=4 SV=1
MM1 pKa = 7.26 TRR3 pKa = 11.84 RR4 pKa = 11.84 TQFKK8 pKa = 9.33 GNSRR12 pKa = 11.84 SRR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 EE17 pKa = 3.62 RR18 pKa = 11.84 LKK20 pKa = 11.17 AKK22 pKa = 10.38 ALANGVLARR31 pKa = 11.84 EE32 pKa = 4.21 EE33 pKa = 4.75 AISSEE38 pKa = 4.23 VLHH41 pKa = 6.83 RR42 pKa = 11.84 PTLSRR47 pKa = 11.84 AQIQAKK53 pKa = 8.11 GTHH56 pKa = 5.29 EE57 pKa = 4.03 TPEE60 pKa = 4.7 RR61 pKa = 11.84 IEE63 pKa = 3.89 DD64 pKa = 3.57 AKK66 pKa = 10.63 PIKK69 pKa = 10.49 FMAQDD74 pKa = 3.8 VIWQQKK80 pKa = 6.5 EE81 pKa = 3.56 YY82 pKa = 10.85 RR83 pKa = 11.84 RR84 pKa = 11.84 NLEE87 pKa = 3.51 RR88 pKa = 11.84 AAIVYY93 pKa = 10.49 ANEE96 pKa = 3.94 FGHH99 pKa = 6.18 RR100 pKa = 11.84 QPEE103 pKa = 4.42 TGVCLPNVALYY114 pKa = 10.32 AAGYY118 pKa = 9.13 RR119 pKa = 11.84 KK120 pKa = 9.93 SKK122 pKa = 10.61 QLTARR127 pKa = 3.76
Molecular weight: 14.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.736
IPC_protein 10.584
Toseland 10.804
ProMoST 10.73
Dawson 10.877
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 11.096
Grimsley 10.921
Solomon 11.023
Lehninger 10.994
Nozaki 10.774
DTASelect 10.599
Thurlkill 10.789
EMBOSS 11.213
Sillero 10.818
Patrickios 10.818
IPC_peptide 11.023
IPC2_peptide 9.487
IPC2.peptide.svr19 8.701
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
65
0
65
12520
29
1183
192.6
21.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.704 ± 0.571
1.286 ± 0.178
5.791 ± 0.27
6.398 ± 0.375
3.379 ± 0.174
7.228 ± 0.305
1.55 ± 0.195
5.551 ± 0.212
5.767 ± 0.289
7.772 ± 0.236
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.668 ± 0.165
4.768 ± 0.244
3.762 ± 0.252
4.696 ± 0.305
5.998 ± 0.325
6.901 ± 0.33
5.895 ± 0.301
6.23 ± 0.268
1.669 ± 0.138
2.987 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here