Cryptobacterium sp. CAG:338

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; Cryptobacterium; environmental samples

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1643 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7CFR9|R7CFR9_9ACTN Thrombospondin type 3 repeat protein OS=Cryptobacterium sp. CAG:338 OX=1262868 GN=BN613_01546 PE=4 SV=1
MM1 pKa = 7.27KK2 pKa = 10.02RR3 pKa = 11.84ASVLVLSLVVALCLALPLAGCSSSSYY29 pKa = 9.98TPQLKK34 pKa = 9.32EE35 pKa = 3.63QSVNDD40 pKa = 3.76SALNSAGTLRR50 pKa = 11.84VGINASNPPYY60 pKa = 10.09AAQSNGSIVGIDD72 pKa = 3.43VDD74 pKa = 3.94IAAALADD81 pKa = 3.88EE82 pKa = 5.39LGLKK86 pKa = 10.48LEE88 pKa = 4.45LVDD91 pKa = 5.2VGTATDD97 pKa = 3.42TAFEE101 pKa = 4.34RR102 pKa = 11.84EE103 pKa = 4.26SVDD106 pKa = 2.49IVMGVEE112 pKa = 4.28EE113 pKa = 4.85ANSAYY118 pKa = 9.33WMSDD122 pKa = 2.33EE123 pKa = 4.45YY124 pKa = 10.59MNSGIALFSLTEE136 pKa = 4.18DD137 pKa = 3.83AEE139 pKa = 4.54APTAAGSFKK148 pKa = 10.76VAAQSSSMSAWEE160 pKa = 4.14VTDD163 pKa = 5.07HH164 pKa = 6.46YY165 pKa = 11.72GEE167 pKa = 4.58SCLEE171 pKa = 4.01NAGDD175 pKa = 3.62PTAAFEE181 pKa = 4.11MLNTGSVNYY190 pKa = 9.61VAAEE194 pKa = 4.11STIGQYY200 pKa = 10.42VIHH203 pKa = 6.91SSGIEE208 pKa = 3.9AYY210 pKa = 9.71PIALLQDD217 pKa = 3.53ATPRR221 pKa = 11.84TVAVAADD228 pKa = 3.67NTEE231 pKa = 3.97LQQAVSNALSALVSGGVVEE250 pKa = 4.75VIEE253 pKa = 4.54GKK255 pKa = 9.36WLGGHH260 pKa = 5.89VDD262 pKa = 3.32ISSLTVIPAPAQEE275 pKa = 4.45STDD278 pKa = 3.67EE279 pKa = 4.19SSEE282 pKa = 4.19DD283 pKa = 4.39ASTTDD288 pKa = 4.05SNTTSDD294 pKa = 3.7TTSDD298 pKa = 3.4TTSGDD303 pKa = 3.52NSSSTSQSPEE313 pKa = 3.9SNTTSEE319 pKa = 4.06

Molecular weight: 32.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7CGG0|R7CGG0_9ACTN Putative peptidoglycan-binding domain-containing protein OS=Cryptobacterium sp. CAG:338 OX=1262868 GN=BN613_01573 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.33QPNTHH10 pKa = 5.99KK11 pKa = 10.54RR12 pKa = 11.84AKK14 pKa = 8.41THH16 pKa = 5.51GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.06SGRR28 pKa = 11.84AVLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1643

0

1643

525131

30

1755

319.6

35.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.496 ± 0.064

1.556 ± 0.028

5.644 ± 0.056

6.974 ± 0.054

4.042 ± 0.042

7.287 ± 0.048

1.947 ± 0.025

6.336 ± 0.054

4.847 ± 0.046

9.305 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.6 ± 0.028

3.764 ± 0.039

3.957 ± 0.036

3.476 ± 0.035

4.855 ± 0.046

6.725 ± 0.055

5.624 ± 0.057

7.512 ± 0.047

0.953 ± 0.023

3.093 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski