Amino acid dipepetide frequency for Cryptobacterium sp. CAG:338

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.811AlaAla: 9.811 ± 0.196	1.67AlaCys: 1.67 ± 0.065	4.825AlaAsp: 4.825 ± 0.115	6.071AlaGlu: 6.071 ± 0.123	3.93AlaPhe: 3.93 ± 0.1	6.865AlaGly: 6.865 ± 0.139	1.897AlaHis: 1.897 ± 0.06	5.759AlaIle: 5.759 ± 0.114	4.641AlaLys: 4.641 ± 0.087	10.253AlaLeu: 10.253 ± 0.195	2.476AlaMet: 2.476 ± 0.075	3.201AlaAsn: 3.201 ± 0.081	3.169AlaPro: 3.169 ± 0.082	4.226AlaGln: 4.226 ± 0.103	4.74AlaArg: 4.74 ± 0.103	6.019AlaSer: 6.019 ± 0.124	5.02AlaThr: 5.02 ± 0.11	6.648AlaVal: 6.648 ± 0.118	0.861AlaTrp: 0.861 ± 0.042	2.868AlaTyr: 2.868 ± 0.081	0.008AlaXaa: 0.008 ± 0.003
Cys	1.586CysAla: 1.586 ± 0.062	0.28CysCys: 0.28 ± 0.023	0.994CysAsp: 0.994 ± 0.048	0.948CysGlu: 0.948 ± 0.043	0.627CysPhe: 0.627 ± 0.035	1.487CysGly: 1.487 ± 0.062	0.312CysHis: 0.312 ± 0.027	0.952CysIle: 0.952 ± 0.05	0.644CysLys: 0.644 ± 0.033	1.343CysLeu: 1.343 ± 0.054	0.404CysMet: 0.404 ± 0.025	0.467CysAsn: 0.467 ± 0.031	0.716CysPro: 0.716 ± 0.042	0.44CysGln: 0.44 ± 0.028	0.628CysArg: 0.628 ± 0.031	1.03CysSer: 1.03 ± 0.043	0.855CysThr: 0.855 ± 0.053	1.247CysVal: 1.247 ± 0.051	0.12CysTrp: 0.12 ± 0.016	0.478CysTyr: 0.478 ± 0.032	0.0CysXaa: 0.0 ± 0.0
Asp	5.561AspAla: 5.561 ± 0.113	0.76AspCys: 0.76 ± 0.039	3.043AspAsp: 3.043 ± 0.09	4.65AspGlu: 4.65 ± 0.105	2.489AspPhe: 2.489 ± 0.066	3.908AspGly: 3.908 ± 0.123	1.021AspHis: 1.021 ± 0.043	3.719AspIle: 3.719 ± 0.085	2.599AspLys: 2.599 ± 0.071	5.237AspLeu: 5.237 ± 0.108	1.457AspMet: 1.457 ± 0.05	2.038AspAsn: 2.038 ± 0.069	2.624AspPro: 2.624 ± 0.077	1.733AspGln: 1.733 ± 0.067	2.603AspArg: 2.603 ± 0.076	3.534AspSer: 3.534 ± 0.087	3.041AspThr: 3.041 ± 0.086	3.793AspVal: 3.793 ± 0.096	0.646AspTrp: 0.646 ± 0.04	1.988AspTyr: 1.988 ± 0.073	0.0AspXaa: 0.0 ± 0.0
Glu	6.652GluAla: 6.652 ± 0.124	0.825GluCys: 0.825 ± 0.042	3.691GluAsp: 3.691 ± 0.087	5.907GluGlu: 5.907 ± 0.134	2.356GluPhe: 2.356 ± 0.075	4.984GluGly: 4.984 ± 0.117	1.482GluHis: 1.482 ± 0.055	4.292GluIle: 4.292 ± 0.098	4.096GluLys: 4.096 ± 0.102	6.198GluLeu: 6.198 ± 0.126	1.944GluMet: 1.944 ± 0.063	2.955GluAsn: 2.955 ± 0.082	2.076GluPro: 2.076 ± 0.058	3.081GluGln: 3.081 ± 0.077	3.599GluArg: 3.599 ± 0.111	3.761GluSer: 3.761 ± 0.09	3.706GluThr: 3.706 ± 0.088	5.452GluVal: 5.452 ± 0.106	0.577GluTrp: 0.577 ± 0.041	2.11GluTyr: 2.11 ± 0.069	0.0GluXaa: 0.0 ± 0.0
Phe	3.77PheAla: 3.77 ± 0.092	0.735PheCys: 0.735 ± 0.043	2.799PheAsp: 2.799 ± 0.078	2.75PheGlu: 2.75 ± 0.064	1.624PhePhe: 1.624 ± 0.067	3.487PheGly: 3.487 ± 0.1	0.649PheHis: 0.649 ± 0.039	2.616PheIle: 2.616 ± 0.081	1.699PheLys: 1.699 ± 0.061	3.432PheLeu: 3.432 ± 0.1	1.074PheMet: 1.074 ± 0.048	1.489PheAsn: 1.489 ± 0.059	1.386PhePro: 1.386 ± 0.051	0.979PheGln: 0.979 ± 0.043	1.588PheArg: 1.588 ± 0.053	3.131PheSer: 3.131 ± 0.091	2.279PheThr: 2.279 ± 0.079	3.31PheVal: 3.31 ± 0.099	0.421PheTrp: 0.421 ± 0.03	1.2PheTyr: 1.2 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	6.332GlyAla: 6.332 ± 0.143	1.344GlyCys: 1.344 ± 0.06	3.675GlyAsp: 3.675 ± 0.115	4.426GlyGlu: 4.426 ± 0.101	3.266GlyPhe: 3.266 ± 0.077	5.225GlyGly: 5.225 ± 0.133	1.367GlyHis: 1.367 ± 0.052	5.682GlyIle: 5.682 ± 0.119	4.066GlyLys: 4.066 ± 0.089	6.555GlyLeu: 6.555 ± 0.125	2.144GlyMet: 2.144 ± 0.071	2.712GlyAsn: 2.712 ± 0.097	1.895GlyPro: 1.895 ± 0.063	2.079GlyGln: 2.079 ± 0.06	3.091GlyArg: 3.091 ± 0.078	5.113GlySer: 5.113 ± 0.098	4.481GlyThr: 4.481 ± 0.118	5.964GlyVal: 5.964 ± 0.123	0.842GlyTrp: 0.842 ± 0.042	2.611GlyTyr: 2.611 ± 0.071	0.0GlyXaa: 0.0 ± 0.0
His	1.84HisAla: 1.84 ± 0.069	0.333HisCys: 0.333 ± 0.029	1.202HisAsp: 1.202 ± 0.054	1.295HisGlu: 1.295 ± 0.048	0.851HisPhe: 0.851 ± 0.039	1.402HisGly: 1.402 ± 0.053	0.51HisHis: 0.51 ± 0.038	1.299HisIle: 1.299 ± 0.053	0.863HisLys: 0.863 ± 0.042	1.691HisLeu: 1.691 ± 0.057	0.508HisMet: 0.508 ± 0.032	0.667HisAsn: 0.667 ± 0.034	1.085HisPro: 1.085 ± 0.048	0.609HisGln: 0.609 ± 0.033	0.948HisArg: 0.948 ± 0.042	1.234HisSer: 1.234 ± 0.05	1.049HisThr: 1.049 ± 0.047	1.283HisVal: 1.283 ± 0.05	0.173HisTrp: 0.173 ± 0.022	0.627HisTyr: 0.627 ± 0.038	0.0HisXaa: 0.0 ± 0.0
Ile	6.918IleAla: 6.918 ± 0.123	1.023IleCys: 1.023 ± 0.046	4.163IleAsp: 4.163 ± 0.085	4.61IleGlu: 4.61 ± 0.103	2.154IlePhe: 2.154 ± 0.082	4.603IleGly: 4.603 ± 0.112	1.202IleHis: 1.202 ± 0.048	3.854IleIle: 3.854 ± 0.12	2.843IleLys: 2.843 ± 0.072	5.193IleLeu: 5.193 ± 0.115	1.619IleMet: 1.619 ± 0.074	2.226IleAsn: 2.226 ± 0.073	3.249IlePro: 3.249 ± 0.079	1.868IleGln: 1.868 ± 0.063	2.649IleArg: 2.649 ± 0.077	4.279IleSer: 4.279 ± 0.09	3.812IleThr: 3.812 ± 0.095	4.968IleVal: 4.968 ± 0.101	0.543IleTrp: 0.543 ± 0.032	1.581IleTyr: 1.581 ± 0.062	0.002IleXaa: 0.002 ± 0.002
Lys	4.744LysAla: 4.744 ± 0.104	0.48LysCys: 0.48 ± 0.037	2.554LysAsp: 2.554 ± 0.076	3.89LysGlu: 3.89 ± 0.1	1.245LysPhe: 1.245 ± 0.054	3.31LysGly: 3.31 ± 0.098	0.939LysHis: 0.939 ± 0.042	2.62LysIle: 2.62 ± 0.072	3.114LysLys: 3.114 ± 0.102	4.016LysLeu: 4.016 ± 0.085	1.264LysMet: 1.264 ± 0.057	2.159LysAsn: 2.159 ± 0.078	2.15LysPro: 2.15 ± 0.066	2.068LysGln: 2.068 ± 0.059	3.102LysArg: 3.102 ± 0.084	2.573LysSer: 2.573 ± 0.074	3.11LysThr: 3.11 ± 0.088	3.502LysVal: 3.502 ± 0.094	0.36LysTrp: 0.36 ± 0.027	1.27LysTyr: 1.27 ± 0.048	0.0LysXaa: 0.0 ± 0.0
Leu	9.051LeuAla: 9.051 ± 0.156	1.541LeuCys: 1.541 ± 0.052	5.503LeuAsp: 5.503 ± 0.107	6.147LeuGlu: 6.147 ± 0.095	3.972LeuPhe: 3.972 ± 0.114	6.88LeuGly: 6.88 ± 0.148	1.744LeuHis: 1.744 ± 0.063	5.698LeuIle: 5.698 ± 0.139	4.258LeuLys: 4.258 ± 0.089	8.541LeuLeu: 8.541 ± 0.169	2.272LeuMet: 2.272 ± 0.075	3.192LeuAsn: 3.192 ± 0.085	4.02LeuPro: 4.02 ± 0.094	2.858LeuGln: 2.858 ± 0.082	4.561LeuArg: 4.561 ± 0.099	7.021LeuSer: 7.021 ± 0.135	4.991LeuThr: 4.991 ± 0.107	7.444LeuVal: 7.444 ± 0.146	0.706LeuTrp: 0.706 ± 0.032	2.647LeuTyr: 2.647 ± 0.076	0.004LeuXaa: 0.004 ± 0.003
Met	2.681MetAla: 2.681 ± 0.076	0.36MetCys: 0.36 ± 0.026	1.383MetAsp: 1.383 ± 0.057	1.577MetGlu: 1.577 ± 0.058	0.853MetPhe: 0.853 ± 0.044	1.921MetGly: 1.921 ± 0.065	0.438MetHis: 0.438 ± 0.03	1.544MetIle: 1.544 ± 0.053	1.464MetLys: 1.464 ± 0.057	2.378MetLeu: 2.378 ± 0.079	0.651MetMet: 0.651 ± 0.042	1.16MetAsn: 1.16 ± 0.044	1.282MetPro: 1.282 ± 0.05	0.847MetGln: 0.847 ± 0.043	1.518MetArg: 1.518 ± 0.05	1.752MetSer: 1.752 ± 0.065	1.562MetThr: 1.562 ± 0.054	1.809MetVal: 1.809 ± 0.05	0.232MetTrp: 0.232 ± 0.022	0.585MetTyr: 0.585 ± 0.035	0.0MetXaa: 0.0 ± 0.0
Asn	3.679AsnAla: 3.679 ± 0.093	0.476AsnCys: 0.476 ± 0.027	2.085AsnAsp: 2.085 ± 0.065	2.62AsnGlu: 2.62 ± 0.074	1.287AsnPhe: 1.287 ± 0.049	2.434AsnGly: 2.434 ± 0.093	0.807AsnHis: 0.807 ± 0.049	2.228AsnIle: 2.228 ± 0.073	1.801AsnLys: 1.801 ± 0.071	3.245AsnLeu: 3.245 ± 0.078	0.996AsnMet: 0.996 ± 0.04	1.661AsnAsn: 1.661 ± 0.069	2.352AsnPro: 2.352 ± 0.067	1.384AsnGln: 1.384 ± 0.054	1.828AsnArg: 1.828 ± 0.058	2.46AsnSer: 2.46 ± 0.076	2.325AsnThr: 2.325 ± 0.089	2.439AsnVal: 2.439 ± 0.078	0.362AsnTrp: 0.362 ± 0.027	1.165AsnTyr: 1.165 ± 0.055	0.002AsnXaa: 0.002 ± 0.002
Pro	3.273ProAla: 3.273 ± 0.087	0.611ProCys: 0.611 ± 0.033	2.432ProAsp: 2.432 ± 0.065	3.607ProGlu: 3.607 ± 0.081	1.744ProPhe: 1.744 ± 0.059	2.757ProGly: 2.757 ± 0.077	0.956ProHis: 0.956 ± 0.045	2.255ProIle: 2.255 ± 0.067	1.803ProLys: 1.803 ± 0.063	3.967ProLeu: 3.967 ± 0.092	0.821ProMet: 0.821 ± 0.042	1.461ProAsn: 1.461 ± 0.057	0.91ProPro: 0.91 ± 0.052	1.474ProGln: 1.474 ± 0.055	1.68ProArg: 1.68 ± 0.063	2.702ProSer: 2.702 ± 0.072	2.251ProThr: 2.251 ± 0.063	2.986ProVal: 2.986 ± 0.07	0.428ProTrp: 0.428 ± 0.03	1.453ProTyr: 1.453 ± 0.055	0.002ProXaa: 0.002 ± 0.002
Gln	3.852GlnAla: 3.852 ± 0.102	0.352GlnCys: 0.352 ± 0.028	1.782GlnAsp: 1.782 ± 0.059	2.506GlnGlu: 2.506 ± 0.074	1.091GlnPhe: 1.091 ± 0.042	2.597GlnGly: 2.597 ± 0.074	0.653GlnHis: 0.653 ± 0.039	2.085GlnIle: 2.085 ± 0.065	1.63GlnLys: 1.63 ± 0.062	2.935GlnLeu: 2.935 ± 0.077	0.935GlnMet: 0.935 ± 0.043	1.501GlnAsn: 1.501 ± 0.061	1.329GlnPro: 1.329 ± 0.064	1.504GlnGln: 1.504 ± 0.086	1.96GlnArg: 1.96 ± 0.067	2.278GlnSer: 2.278 ± 0.074	1.977GlnThr: 1.977 ± 0.062	2.567GlnVal: 2.567 ± 0.069	0.366GlnTrp: 0.366 ± 0.029	0.863GlnTyr: 0.863 ± 0.04	0.002GlnXaa: 0.002 ± 0.002
Arg	4.203ArgAla: 4.203 ± 0.101	0.756ArgCys: 0.756 ± 0.043	2.577ArgAsp: 2.577 ± 0.078	3.633ArgGlu: 3.633 ± 0.095	2.182ArgPhe: 2.182 ± 0.08	2.906ArgGly: 2.906 ± 0.078	0.948ArgHis: 0.948 ± 0.041	3.504ArgIle: 3.504 ± 0.073	2.792ArgLys: 2.792 ± 0.067	4.182ArgLeu: 4.182 ± 0.109	1.571ArgMet: 1.571 ± 0.063	1.935ArgAsn: 1.935 ± 0.06	1.634ArgPro: 1.634 ± 0.056	1.594ArgGln: 1.594 ± 0.057	2.744ArgArg: 2.744 ± 0.078	3.034ArgSer: 3.034 ± 0.088	2.567ArgThr: 2.567 ± 0.073	3.473ArgVal: 3.473 ± 0.085	0.486ArgTrp: 0.486 ± 0.03	1.834ArgTyr: 1.834 ± 0.065	0.0ArgXaa: 0.0 ± 0.0
Ser	5.939SerAla: 5.939 ± 0.131	0.977SerCys: 0.977 ± 0.047	3.894SerAsp: 3.894 ± 0.089	4.254SerGlu: 4.254 ± 0.106	3.211SerPhe: 3.211 ± 0.083	5.123SerGly: 5.123 ± 0.123	1.259SerHis: 1.259 ± 0.054	4.142SerIle: 4.142 ± 0.104	2.948SerLys: 2.948 ± 0.093	6.655SerLeu: 6.655 ± 0.137	1.693SerMet: 1.693 ± 0.06	2.485SerAsn: 2.485 ± 0.078	2.346SerPro: 2.346 ± 0.059	2.468SerGln: 2.468 ± 0.076	3.146SerArg: 3.146 ± 0.09	5.431SerSer: 5.431 ± 0.15	3.597SerThr: 3.597 ± 0.098	4.749SerVal: 4.749 ± 0.104	0.682SerTrp: 0.682 ± 0.041	2.243SerTyr: 2.243 ± 0.079	0.006SerXaa: 0.006 ± 0.003
Thr	4.536ThrAla: 4.536 ± 0.09	0.916ThrCys: 0.916 ± 0.052	3.072ThrAsp: 3.072 ± 0.09	3.386ThrGlu: 3.386 ± 0.089	2.443ThrPhe: 2.443 ± 0.086	4.416ThrGly: 4.416 ± 0.103	1.144ThrHis: 1.144 ± 0.047	3.892ThrIle: 3.892 ± 0.103	2.586ThrLys: 2.586 ± 0.064	5.492ThrLeu: 5.492 ± 0.112	1.335ThrMet: 1.335 ± 0.05	2.135ThrAsn: 2.135 ± 0.066	2.683ThrPro: 2.683 ± 0.073	1.92ThrGln: 1.92 ± 0.069	2.382ThrArg: 2.382 ± 0.06	3.782ThrSer: 3.782 ± 0.09	3.302ThrThr: 3.302 ± 0.102	4.237ThrVal: 4.237 ± 0.126	0.627ThrTrp: 0.627 ± 0.036	1.952ThrTyr: 1.952 ± 0.074	0.002ThrXaa: 0.002 ± 0.002
Val	7.0ValAla: 7.0 ± 0.131	1.358ValCys: 1.358 ± 0.053	4.201ValAsp: 4.201 ± 0.09	4.757ValGlu: 4.757 ± 0.105	3.293ValPhe: 3.293 ± 0.086	5.361ValGly: 5.361 ± 0.11	1.297ValHis: 1.297 ± 0.058	4.963ValIle: 4.963 ± 0.097	2.98ValLys: 2.98 ± 0.077	7.674ValLeu: 7.674 ± 0.157	1.904ValMet: 1.904 ± 0.07	2.63ValAsn: 2.63 ± 0.071	3.112ValPro: 3.112 ± 0.09	2.173ValGln: 2.173 ± 0.074	3.504ValArg: 3.504 ± 0.093	5.431ValSer: 5.431 ± 0.095	4.138ValThr: 4.138 ± 0.102	6.56ValVal: 6.56 ± 0.16	0.678ValTrp: 0.678 ± 0.036	2.108ValTyr: 2.108 ± 0.06	0.002ValXaa: 0.002 ± 0.002
Trp	0.792TrpAla: 0.792 ± 0.041	0.173TrpCys: 0.173 ± 0.019	0.548TrpAsp: 0.548 ± 0.04	0.56TrpGlu: 0.56 ± 0.037	0.427TrpPhe: 0.427 ± 0.028	0.756TrpGly: 0.756 ± 0.038	0.183TrpHis: 0.183 ± 0.02	0.621TrpIle: 0.621 ± 0.04	0.444TrpLys: 0.444 ± 0.033	0.998TrpLeu: 0.998 ± 0.051	0.295TrpMet: 0.295 ± 0.029	0.472TrpAsn: 0.472 ± 0.029	0.274TrpPro: 0.274 ± 0.021	0.316TrpGln: 0.316 ± 0.024	0.489TrpArg: 0.489 ± 0.03	0.613TrpSer: 0.613 ± 0.037	0.461TrpThr: 0.461 ± 0.039	0.674TrpVal: 0.674 ± 0.042	0.116TrpTrp: 0.116 ± 0.014	0.312TrpTyr: 0.312 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.735TyrAla: 2.735 ± 0.07	0.587TyrCys: 0.587 ± 0.035	2.019TyrAsp: 2.019 ± 0.083	2.146TyrGlu: 2.146 ± 0.071	1.371TyrPhe: 1.371 ± 0.05	2.445TyrGly: 2.445 ± 0.071	0.661TyrHis: 0.661 ± 0.034	1.63TyrIle: 1.63 ± 0.057	1.198TyrLys: 1.198 ± 0.055	3.066TyrLeu: 3.066 ± 0.076	0.63TyrMet: 0.63 ± 0.037	1.095TyrAsn: 1.095 ± 0.053	1.28TyrPro: 1.28 ± 0.05	1.143TyrGln: 1.143 ± 0.051	1.792TyrArg: 1.792 ± 0.06	2.07TyrSer: 2.07 ± 0.065	1.716TyrThr: 1.716 ± 0.071	2.011TyrVal: 2.011 ± 0.06	0.301TyrTrp: 0.301 ± 0.026	1.038TyrTyr: 1.038 ± 0.051	0.002TyrXaa: 0.002 ± 0.002
Xaa	0.0XaaAla: 0.0 ± 0.0	0.002XaaCys: 0.002 ± 0.002	0.002XaaAsp: 0.002 ± 0.002	0.002XaaGlu: 0.002 ± 0.002	0.002XaaPhe: 0.002 ± 0.002	0.004XaaGly: 0.004 ± 0.003	0.0XaaHis: 0.0 ± 0.0	0.002XaaIle: 0.002 ± 0.002	0.002XaaLys: 0.002 ± 0.002	0.002XaaLeu: 0.002 ± 0.002	0.002XaaMet: 0.002 ± 0.002	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.002	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.006XaaVal: 0.006 ± 0.003	0.0XaaTrp: 0.0 ± 0.0	0.002XaaTyr: 0.002 ± 0.002	0.038XaaXaa: 0.038 ± 0.012

Statistics based on 1643 proteins (525131 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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