Torque teno felis virus
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D5BV89|A0A0D5BV89_9VIRU Capsid protein OS=Torque teno felis virus OX=687384 PE=3 SV=1
VV1 pKa = 6.26 MGSSKK6 pKa = 9.25 TRR8 pKa = 11.84 LMNEE12 pKa = 3.75 LLEE15 pKa = 4.81 IIQALSGPDD24 pKa = 3.16 PWEE27 pKa = 4.19 SDD29 pKa = 2.93 GGMGPQGEE37 pKa = 4.25 PSKK40 pKa = 11.33 SNSRR44 pKa = 11.84 LLYY47 pKa = 10.24 SDD49 pKa = 5.45 PDD51 pKa = 4.19 PEE53 pKa = 5.97 DD54 pKa = 3.74 SSSDD58 pKa = 3.68 DD59 pKa = 3.58 SEE61 pKa = 3.9 MSYY64 pKa = 10.96 FSSSDD69 pKa = 3.3 STDD72 pKa = 3.59 ADD74 pKa = 3.16 ITNPNMEE81 pKa = 4.76 GGRR84 pKa = 11.84 TPPRR88 pKa = 11.84 YY89 pKa = 9.09 PPRR92 pKa = 11.84 TWAFTFKK99 pKa = 10.95
Molecular weight: 10.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.366
IPC2_protein 4.113
IPC_protein 4.037
Toseland 3.846
ProMoST 4.202
Dawson 4.024
Bjellqvist 4.177
Wikipedia 3.948
Rodwell 3.872
Grimsley 3.757
Solomon 4.012
Lehninger 3.973
Nozaki 4.139
DTASelect 4.355
Thurlkill 3.897
EMBOSS 3.961
Sillero 4.164
Patrickios 3.897
IPC_peptide 4.012
IPC2_peptide 4.139
IPC2.peptide.svr19 4.076
Protein with the highest isoelectric point:
>tr|A0A0D5BV89|A0A0D5BV89_9VIRU Capsid protein OS=Torque teno felis virus OX=687384 PE=3 SV=1
MM1 pKa = 6.38 YY2 pKa = 9.92 QGRR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 WGRR14 pKa = 11.84 RR15 pKa = 11.84 VLSHH19 pKa = 6.66 YY20 pKa = 8.89 KK21 pKa = 10.08 RR22 pKa = 11.84 KK23 pKa = 8.33 WRR25 pKa = 11.84 RR26 pKa = 11.84 GSRR29 pKa = 11.84 RR30 pKa = 11.84 GHH32 pKa = 6.11 GIYY35 pKa = 10.12 RR36 pKa = 11.84 RR37 pKa = 11.84 WRR39 pKa = 11.84 RR40 pKa = 11.84 WRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 PRR46 pKa = 11.84 TVVTEE51 pKa = 3.91 QHH53 pKa = 5.75 SKK55 pKa = 9.79 RR56 pKa = 11.84 VKK58 pKa = 9.17 TIIVRR63 pKa = 11.84 GWEE66 pKa = 3.94 PLGNICPTDD75 pKa = 3.53 SAKK78 pKa = 10.95 AKK80 pKa = 6.83 ATPYY84 pKa = 10.93 ASYY87 pKa = 11.07 DD88 pKa = 3.47 SDD90 pKa = 3.65 SGQGQWHH97 pKa = 5.87 GTWGHH102 pKa = 5.63 HH103 pKa = 4.66 WFTFQSLVDD112 pKa = 3.63 RR113 pKa = 11.84 AEE115 pKa = 3.8 ARR117 pKa = 11.84 LNSFSGNWEE126 pKa = 3.92 SYY128 pKa = 10.83 DD129 pKa = 3.5 YY130 pKa = 11.52 LRR132 pKa = 11.84 FLGGTMYY139 pKa = 11.33 LMQPRR144 pKa = 11.84 EE145 pKa = 3.91 MCFMFGNDD153 pKa = 4.22 PYY155 pKa = 11.86 LMTSDD160 pKa = 4.11 LDD162 pKa = 3.64 KK163 pKa = 10.85 TASQKK168 pKa = 10.16 NRR170 pKa = 11.84 AEE172 pKa = 4.35 EE173 pKa = 3.9 TWITPGYY180 pKa = 9.47 LIHH183 pKa = 7.6 RR184 pKa = 11.84 PGTHH188 pKa = 7.54 LILSRR193 pKa = 11.84 QKK195 pKa = 9.31 VEE197 pKa = 3.63 RR198 pKa = 11.84 RR199 pKa = 11.84 SMYY202 pKa = 10.04 KK203 pKa = 9.58 IRR205 pKa = 11.84 VPVPTSWRR213 pKa = 11.84 GWFPIPEE220 pKa = 4.32 CFSYY224 pKa = 10.5 VLCHH228 pKa = 7.11 WYY230 pKa = 8.44 RR231 pKa = 11.84 TWWDD235 pKa = 3.18 PDD237 pKa = 3.07 ACFFDD242 pKa = 4.19 PCATGSSCEE251 pKa = 3.99 AEE253 pKa = 4.5 PWWSTAHH260 pKa = 6.38 TKK262 pKa = 8.74 QAWVDD267 pKa = 3.57 RR268 pKa = 11.84 TKK270 pKa = 11.23 LDD272 pKa = 3.96 DD273 pKa = 4.54 PPVGGIGPNQKK284 pKa = 7.54 TWAPFLPSRR293 pKa = 11.84 PCTNYY298 pKa = 8.33 YY299 pKa = 8.02 THH301 pKa = 7.08 SASFWFKK308 pKa = 11.17 YY309 pKa = 9.82 KK310 pKa = 10.85 LKK312 pKa = 10.6 FQVTGEE318 pKa = 4.46 NIWAPVPRR326 pKa = 11.84 DD327 pKa = 3.29 YY328 pKa = 11.19 SQQGTVPTAPSRR340 pKa = 11.84 QQVEE344 pKa = 4.36 SQARR348 pKa = 11.84 AAYY351 pKa = 9.9 SKK353 pKa = 11.17 ANRR356 pKa = 11.84 PPTTADD362 pKa = 3.45 ILPGDD367 pKa = 4.5 LDD369 pKa = 3.8 SDD371 pKa = 4.87 GILKK375 pKa = 10.64 DD376 pKa = 3.4 EE377 pKa = 4.56 AYY379 pKa = 10.44 EE380 pKa = 4.39 RR381 pKa = 11.84 ITRR384 pKa = 11.84 NNPSPKK390 pKa = 9.25 RR391 pKa = 11.84 PRR393 pKa = 11.84 PVGIRR398 pKa = 11.84 WRR400 pKa = 11.84 DD401 pKa = 3.47 GTPGRR406 pKa = 11.84 TLQEE410 pKa = 3.87 QQQAAVLRR418 pKa = 11.84 PRR420 pKa = 11.84 PRR422 pKa = 11.84 RR423 pKa = 11.84 QLLRR427 pKa = 11.84 RR428 pKa = 11.84 LRR430 pKa = 11.84 NVLLQLL436 pKa = 3.99
Molecular weight: 51.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 9.75
IPC_protein 10.643
Toseland 10.526
ProMoST 10.306
Dawson 10.687
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 10.818
Grimsley 10.76
Solomon 10.774
Lehninger 10.73
Nozaki 10.526
DTASelect 10.423
Thurlkill 10.555
EMBOSS 10.935
Sillero 10.613
Patrickios 10.423
IPC_peptide 10.774
IPC2_peptide 9.56
IPC2.peptide.svr19 8.452
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
640
99
436
213.3
24.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.156 ± 0.678
1.719 ± 0.487
6.563 ± 1.789
5.313 ± 1.727
3.281 ± 0.139
7.187 ± 0.549
2.5 ± 0.772
3.281 ± 0.163
4.219 ± 0.325
6.25 ± 0.11
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.188 ± 0.819
2.656 ± 0.5
7.813 ± 1.181
4.375 ± 0.771
10.156 ± 2.75
8.906 ± 2.642
6.094 ± 0.92
4.375 ± 1.066
4.063 ± 1.008
3.906 ± 0.462
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here