Paenalcaligenes hominis
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2082 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1U9JZY0|A0A1U9JZY0_9BURK Uncharacterized protein OS=Paenalcaligenes hominis OX=643674 GN=PAEH1_06790 PE=4 SV=1
MM1 pKa = 6.77 STWMCLICGWIYY13 pKa = 11.01 DD14 pKa = 4.19 EE15 pKa = 4.49 EE16 pKa = 6.25 AGLPEE21 pKa = 4.49 EE22 pKa = 5.51 GIAPGTKK29 pKa = 9.07 WEE31 pKa = 4.43 DD32 pKa = 3.47 VPPNWVCPEE41 pKa = 3.8 CGARR45 pKa = 11.84 KK46 pKa = 9.53 EE47 pKa = 4.01 DD48 pKa = 3.72 FEE50 pKa = 4.59 MIEE53 pKa = 4.03 VV54 pKa = 3.56
Molecular weight: 6.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.829
IPC2_protein 3.986
IPC_protein 3.795
Toseland 3.643
ProMoST 3.935
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.643
Rodwell 3.643
Grimsley 3.579
Solomon 3.719
Lehninger 3.668
Nozaki 3.897
DTASelect 3.948
Thurlkill 3.694
EMBOSS 3.656
Sillero 3.91
Patrickios 0.604
IPC_peptide 3.719
IPC2_peptide 3.884
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|A0A1U9K0K4|A0A1U9K0K4_9BURK DNA polymerase IV OS=Paenalcaligenes hominis OX=643674 GN=dinB PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.26 RR14 pKa = 11.84 THH16 pKa = 5.86 GFRR19 pKa = 11.84 LRR21 pKa = 11.84 MKK23 pKa = 9.28 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.65 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2082
0
2082
604123
37
1957
290.2
32.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.475 ± 0.06
0.901 ± 0.018
5.092 ± 0.042
5.599 ± 0.056
3.741 ± 0.034
6.935 ± 0.05
2.665 ± 0.033
5.613 ± 0.042
3.831 ± 0.042
10.988 ± 0.07
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.453 ± 0.025
3.338 ± 0.033
4.623 ± 0.034
5.332 ± 0.048
5.467 ± 0.041
5.892 ± 0.043
5.53 ± 0.034
7.288 ± 0.045
1.451 ± 0.028
2.786 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here