Beihai sobemo-like virus 13
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KF26|A0A1L3KF26_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 13 OX=1922684 PE=4 SV=1
MM1 pKa = 7.73 LDD3 pKa = 3.66 DD4 pKa = 6.23 LEE6 pKa = 5.36 TFTDD10 pKa = 5.07 WINQQNRR17 pKa = 11.84 EE18 pKa = 4.11 TMRR21 pKa = 11.84 MDD23 pKa = 4.69 PDD25 pKa = 3.81 SSPGIPWHH33 pKa = 6.43 SAGSTNGEE41 pKa = 3.65 IFDD44 pKa = 4.04 YY45 pKa = 11.36 VPGFGYY51 pKa = 10.57 NKK53 pKa = 9.81 NRR55 pKa = 11.84 LEE57 pKa = 4.07 EE58 pKa = 3.79 LRR60 pKa = 11.84 LAVKK64 pKa = 10.08 QRR66 pKa = 11.84 LVEE69 pKa = 4.15 LEE71 pKa = 4.11 VGSALDD77 pKa = 4.67 PISLFIKK84 pKa = 10.44 QEE86 pKa = 3.63 YY87 pKa = 9.18 HH88 pKa = 5.31 KK89 pKa = 10.62 QKK91 pKa = 10.76 KK92 pKa = 9.69 ADD94 pKa = 3.59 EE95 pKa = 4.22 EE96 pKa = 4.02 RR97 pKa = 11.84 WRR99 pKa = 11.84 LISSVGLTDD108 pKa = 3.66 QIVARR113 pKa = 11.84 MLFEE117 pKa = 4.52 PFFDD121 pKa = 3.98 YY122 pKa = 11.17 VLARR126 pKa = 11.84 PMQFRR131 pKa = 11.84 MAIGWGLTAPGSLTFMNAHH150 pKa = 7.33 IGPDD154 pKa = 3.56 PVEE157 pKa = 4.52 AADD160 pKa = 3.86 KK161 pKa = 10.97 SAWDD165 pKa = 3.43 WTVQPWLFDD174 pKa = 3.33 VFYY177 pKa = 9.91 NICLALFDD185 pKa = 5.04 SRR187 pKa = 11.84 DD188 pKa = 3.66 DD189 pKa = 3.53 RR190 pKa = 11.84 FKK192 pKa = 10.79 TIMRR196 pKa = 11.84 HH197 pKa = 4.57 HH198 pKa = 6.82 LRR200 pKa = 11.84 AICYY204 pKa = 10.15 DD205 pKa = 3.23 KK206 pKa = 11.5 VFDD209 pKa = 3.97 INGRR213 pKa = 11.84 RR214 pKa = 11.84 YY215 pKa = 9.35 RR216 pKa = 11.84 QEE218 pKa = 3.93 LPGIMPSGWFMTILFNSVSQLILHH242 pKa = 7.23 AYY244 pKa = 9.77 ADD246 pKa = 4.71 DD247 pKa = 4.25 GDD249 pKa = 4.04 EE250 pKa = 4.27 FAWPLVMGDD259 pKa = 4.62 DD260 pKa = 4.8 TIQPQASPQYY270 pKa = 8.25 WDD272 pKa = 4.42 RR273 pKa = 11.84 IRR275 pKa = 11.84 KK276 pKa = 6.7 TGCILKK282 pKa = 9.84 EE283 pKa = 3.94 LSNEE287 pKa = 3.77 RR288 pKa = 11.84 DD289 pKa = 3.15 FCGFNFSPEE298 pKa = 3.96 AYY300 pKa = 10.25 LPVYY304 pKa = 9.12 GLKK307 pKa = 10.17 YY308 pKa = 10.66 DD309 pKa = 3.97 FAIHH313 pKa = 6.52 HH314 pKa = 6.71 IEE316 pKa = 3.92 PSVLKK321 pKa = 9.12 EE322 pKa = 4.03 TLASFQWLYY331 pKa = 11.72 AFVPQQLSSIQRR343 pKa = 11.84 YY344 pKa = 8.0 IRR346 pKa = 11.84 EE347 pKa = 3.68 IGYY350 pKa = 10.29 ADD352 pKa = 3.91 LVVRR356 pKa = 11.84 PQDD359 pKa = 3.22 MQRR362 pKa = 11.84 RR363 pKa = 11.84 VLGLPVRR370 pKa = 11.84 MPRR373 pKa = 11.84 LL374 pKa = 3.42
Molecular weight: 43.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.277
IPC2_protein 5.296
IPC_protein 5.283
Toseland 5.385
ProMoST 5.499
Dawson 5.372
Bjellqvist 5.461
Wikipedia 5.296
Rodwell 5.296
Grimsley 5.385
Solomon 5.372
Lehninger 5.334
Nozaki 5.537
DTASelect 5.728
Thurlkill 5.461
EMBOSS 5.448
Sillero 5.614
Patrickios 4.101
IPC_peptide 5.385
IPC2_peptide 5.614
IPC2.peptide.svr19 5.573
Protein with the highest isoelectric point:
>tr|A0A1L3KF26|A0A1L3KF26_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 13 OX=1922684 PE=4 SV=1
MM1 pKa = 7.25 VVIMTHH7 pKa = 6.94 FYY9 pKa = 11.24 QNLTYY14 pKa = 10.59 FLNTDD19 pKa = 4.16 LAVGYY24 pKa = 9.99 IIGLIVFLLLEE35 pKa = 4.26 YY36 pKa = 10.15 RR37 pKa = 11.84 PKK39 pKa = 10.43 IFGPHH44 pKa = 6.34 RR45 pKa = 11.84 MPSLFYY51 pKa = 10.66 NLKK54 pKa = 10.35 LLFWPSSVALLRR66 pKa = 11.84 LAAIALLWILQPYY79 pKa = 10.08 ALTNVYY85 pKa = 10.21 FEE87 pKa = 4.45 EE88 pKa = 4.79 ALLYY92 pKa = 10.48 LPQGTEE98 pKa = 3.85 GAFFGFRR105 pKa = 11.84 FFFLMAGIVWTVKK118 pKa = 8.67 TAYY121 pKa = 9.71 VSLRR125 pKa = 11.84 ILVGGLEE132 pKa = 4.0 VAAYY136 pKa = 9.4 YY137 pKa = 10.63 SSSVDD142 pKa = 3.36 LSVPAQITVSSDD154 pKa = 2.64 KK155 pKa = 10.78 SRR157 pKa = 11.84 SSVAVGVYY165 pKa = 10.14 VDD167 pKa = 5.71 GDD169 pKa = 3.94 FEE171 pKa = 5.93 SNGQATCVAVEE182 pKa = 3.93 SRR184 pKa = 11.84 RR185 pKa = 11.84 EE186 pKa = 3.77 DD187 pKa = 4.0 GIADD191 pKa = 3.68 EE192 pKa = 6.01 FITATHH198 pKa = 5.91 VVTDD202 pKa = 4.79 LDD204 pKa = 4.44 LSNLVIWRR212 pKa = 11.84 AGVALRR218 pKa = 11.84 VQSVLQPDD226 pKa = 4.05 PNGLHH231 pKa = 7.47 DD232 pKa = 3.67 IAILRR237 pKa = 11.84 VRR239 pKa = 11.84 HH240 pKa = 5.03 GASRR244 pKa = 11.84 LGVRR248 pKa = 11.84 PADD251 pKa = 3.69 LGLFPQTTGTASLHH265 pKa = 5.15 CVQRR269 pKa = 11.84 EE270 pKa = 4.29 VIAKK274 pKa = 8.04 TVGSVTPGSTRR285 pKa = 11.84 YY286 pKa = 10.25 SNYY289 pKa = 10.42 AFTGNTKK296 pKa = 10.48 AGWSGAGLYY305 pKa = 10.51 DD306 pKa = 3.24 VTGRR310 pKa = 11.84 FIGMHH315 pKa = 5.26 TCFLGDD321 pKa = 3.27 TSKK324 pKa = 10.45 MVNGGYY330 pKa = 10.33 NLVPIMDD337 pKa = 3.65 HH338 pKa = 4.87 VLRR341 pKa = 11.84 YY342 pKa = 9.85 KK343 pKa = 10.44 PLNKK347 pKa = 9.97 NCASCDD353 pKa = 3.5 SKK355 pKa = 11.19 LTMLRR360 pKa = 11.84 IRR362 pKa = 11.84 EE363 pKa = 4.06 GLMEE367 pKa = 5.54 DD368 pKa = 4.52 FVTVNPKK375 pKa = 10.28 SKK377 pKa = 10.73 DD378 pKa = 3.32 YY379 pKa = 10.91 GVCNEE384 pKa = 4.26 CSGLEE389 pKa = 4.0 KK390 pKa = 10.8 KK391 pKa = 10.06 KK392 pKa = 11.04 GKK394 pKa = 9.52 NKK396 pKa = 9.9 KK397 pKa = 9.21 KK398 pKa = 10.29 RR399 pKa = 11.84 RR400 pKa = 11.84 TGGTWGWLDD409 pKa = 3.69 EE410 pKa = 5.1 KK411 pKa = 10.82 ILSGKK416 pKa = 10.33 AKK418 pKa = 9.56 IRR420 pKa = 11.84 KK421 pKa = 8.39 VPWGLGEE428 pKa = 3.79 WLYY431 pKa = 10.91 EE432 pKa = 4.06 VEE434 pKa = 4.47 TEE436 pKa = 4.23 DD437 pKa = 4.11 GTVEE441 pKa = 4.06 TLTEE445 pKa = 4.15 DD446 pKa = 3.54 EE447 pKa = 4.33 AVNRR451 pKa = 11.84 LSKK454 pKa = 10.46 AACRR458 pKa = 11.84 KK459 pKa = 10.05 LGIFKK464 pKa = 10.31 SDD466 pKa = 3.13 RR467 pKa = 11.84 PTDD470 pKa = 3.39 EE471 pKa = 6.11 DD472 pKa = 4.18 EE473 pKa = 4.42 YY474 pKa = 11.19 QEE476 pKa = 4.23 EE477 pKa = 4.37 RR478 pKa = 11.84 NDD480 pKa = 3.14 FHH482 pKa = 9.46 DD483 pKa = 3.75 YY484 pKa = 9.67 MDD486 pKa = 4.79 RR487 pKa = 11.84 EE488 pKa = 4.24 RR489 pKa = 11.84 FDD491 pKa = 3.14 PWAHH495 pKa = 7.32 LEE497 pKa = 4.02 RR498 pKa = 11.84 QKK500 pKa = 10.85 NQKK503 pKa = 9.43 NLSDD507 pKa = 4.05 SKK509 pKa = 11.04 KK510 pKa = 10.38 SSQSQQEE517 pKa = 4.0 AGKK520 pKa = 10.37 GSPTIKK526 pKa = 10.25 PLPRR530 pKa = 11.84 PSAPVALPSLDD541 pKa = 3.37 EE542 pKa = 3.86 ARR544 pKa = 11.84 RR545 pKa = 11.84 AVEE548 pKa = 3.87 EE549 pKa = 3.98 NEE551 pKa = 3.95 EE552 pKa = 4.19 VVRR555 pKa = 11.84 QVSVVIRR562 pKa = 11.84 ALAEE566 pKa = 3.84 AQLRR570 pKa = 11.84 ARR572 pKa = 11.84 RR573 pKa = 11.84 AWEE576 pKa = 3.77 ADD578 pKa = 3.03 EE579 pKa = 6.64 SEE581 pKa = 4.59 DD582 pKa = 3.1 HH583 pKa = 6.3 WKK585 pKa = 10.74 AILQLSVDD593 pKa = 3.41 THH595 pKa = 6.41 ALQNFLADD603 pKa = 3.94 VKK605 pKa = 11.09 DD606 pKa = 3.93 SNQDD610 pKa = 2.74 ITKK613 pKa = 9.76 LWNQISQGSEE623 pKa = 3.2 IDD625 pKa = 3.52 VSLHH629 pKa = 5.35 KK630 pKa = 10.37 RR631 pKa = 11.84 VEE633 pKa = 3.77 RR634 pKa = 11.84 RR635 pKa = 11.84 RR636 pKa = 11.84 TKK638 pKa = 10.17 TKK640 pKa = 10.03 EE641 pKa = 3.84 KK642 pKa = 9.9 PPPSPEE648 pKa = 3.46 ILQMLGRR655 pKa = 11.84 ADD657 pKa = 3.82 TVHH660 pKa = 6.98 ASALTTLVNIPKK672 pKa = 10.16 RR673 pKa = 11.84 LRR675 pKa = 11.84 PTAGQEE681 pKa = 3.99 NLSGDD686 pKa = 4.03 MIQYY690 pKa = 9.34 LGKK693 pKa = 10.45 YY694 pKa = 7.75 GVKK697 pKa = 10.07 IADD700 pKa = 4.14 DD701 pKa = 4.04 PFNTPEE707 pKa = 4.53 DD708 pKa = 3.65 SLNSVRR714 pKa = 11.84 AATGGPQQPEE724 pKa = 3.96 VSKK727 pKa = 10.65 PSTSTSQSEE736 pKa = 4.45 STRR739 pKa = 11.84 TTRR742 pKa = 11.84 SNSSQGKK749 pKa = 8.76 KK750 pKa = 9.6 KK751 pKa = 10.58 KK752 pKa = 9.04 KK753 pKa = 8.45 TSQDD757 pKa = 3.12 TSLKK761 pKa = 8.77 TSKK764 pKa = 9.55 TVEE767 pKa = 4.27 DD768 pKa = 3.7 SSNPQKK774 pKa = 11.25 LEE776 pKa = 3.59 IPKK779 pKa = 9.88 LLKK782 pKa = 10.07 PAFEE786 pKa = 5.55 SILSINKK793 pKa = 7.91 EE794 pKa = 3.78 QQEE797 pKa = 4.06 QLNRR801 pKa = 11.84 QLQEE805 pKa = 3.69 IAPEE809 pKa = 3.87 LLEE812 pKa = 4.33 EE813 pKa = 4.63 FSHH816 pKa = 6.63 FFGRR820 pKa = 11.84 FEE822 pKa = 4.14 DD823 pKa = 3.85 TT824 pKa = 4.02
Molecular weight: 92.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.879
IPC2_protein 7.322
IPC_protein 7.249
Toseland 7.117
ProMoST 7.761
Dawson 7.951
Bjellqvist 8.17
Wikipedia 7.892
Rodwell 7.98
Grimsley 7.205
Solomon 8.024
Lehninger 8.039
Nozaki 8.361
DTASelect 8.039
Thurlkill 8.112
EMBOSS 8.185
Sillero 8.375
Patrickios 4.622
IPC_peptide 8.024
IPC2_peptide 7.176
IPC2.peptide.svr19 7.41
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1198
374
824
599.0
67.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.678 ± 0.281
1.002 ± 0.036
6.177 ± 0.839
6.344 ± 0.53
4.424 ± 0.918
6.26 ± 0.343
1.92 ± 0.117
5.008 ± 0.75
5.593 ± 1.126
9.933 ± 0.164
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.087 ± 0.739
3.589 ± 0.345
5.175 ± 0.519
4.591 ± 0.26
6.427 ± 0.421
7.429 ± 0.965
5.509 ± 1.082
6.678 ± 0.708
2.003 ± 0.499
3.172 ± 0.446
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here