Amino acid dipepetide frequency for Streptomyces sp. JV178

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.315AlaAla: 20.315 ± 0.129	1.055AlaCys: 1.055 ± 0.021	8.38AlaAsp: 8.38 ± 0.065	8.757AlaGlu: 8.757 ± 0.064	3.457AlaPhe: 3.457 ± 0.035	12.562AlaGly: 12.562 ± 0.074	2.948AlaHis: 2.948 ± 0.041	3.39AlaIle: 3.39 ± 0.037	2.822AlaLys: 2.822 ± 0.043	14.397AlaLeu: 14.397 ± 0.094	2.324AlaMet: 2.324 ± 0.032	1.982AlaAsn: 1.982 ± 0.031	7.227AlaPro: 7.227 ± 0.065	3.524AlaGln: 3.524 ± 0.038	10.205AlaArg: 10.205 ± 0.067	5.879AlaSer: 5.879 ± 0.05	7.147AlaThr: 7.147 ± 0.057	12.021AlaVal: 12.021 ± 0.077	1.868AlaTrp: 1.868 ± 0.028	2.836AlaTyr: 2.836 ± 0.035	0.0AlaXaa: 0.0 ± 0.0
Cys	1.07CysAla: 1.07 ± 0.018	0.095CysCys: 0.095 ± 0.007	0.485CysAsp: 0.485 ± 0.011	0.406CysGlu: 0.406 ± 0.012	0.23CysPhe: 0.23 ± 0.008	0.919CysGly: 0.919 ± 0.018	0.203CysHis: 0.203 ± 0.008	0.139CysIle: 0.139 ± 0.006	0.119CysLys: 0.119 ± 0.007	0.774CysLeu: 0.774 ± 0.016	0.122CysMet: 0.122 ± 0.006	0.133CysAsn: 0.133 ± 0.007	0.459CysPro: 0.459 ± 0.013	0.166CysGln: 0.166 ± 0.007	0.594CysArg: 0.594 ± 0.015	0.404CysSer: 0.404 ± 0.012	0.491CysThr: 0.491 ± 0.013	0.696CysVal: 0.696 ± 0.017	0.136CysTrp: 0.136 ± 0.007	0.146CysTyr: 0.146 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	7.732AspAla: 7.732 ± 0.059	0.435AspCys: 0.435 ± 0.013	3.883AspAsp: 3.883 ± 0.042	3.947AspGlu: 3.947 ± 0.039	1.726AspPhe: 1.726 ± 0.028	6.718AspGly: 6.718 ± 0.058	1.479AspHis: 1.479 ± 0.024	1.972AspIle: 1.972 ± 0.026	1.227AspLys: 1.227 ± 0.026	6.335AspLeu: 6.335 ± 0.049	0.833AspMet: 0.833 ± 0.018	1.007AspAsn: 1.007 ± 0.022	4.599AspPro: 4.599 ± 0.047	1.536AspGln: 1.536 ± 0.026	5.033AspArg: 5.033 ± 0.045	2.639AspSer: 2.639 ± 0.036	3.52AspThr: 3.52 ± 0.033	4.779AspVal: 4.779 ± 0.043	1.092AspTrp: 1.092 ± 0.02	1.153AspTyr: 1.153 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	7.422GluAla: 7.422 ± 0.064	0.357GluCys: 0.357 ± 0.011	2.891GluAsp: 2.891 ± 0.035	3.597GluGlu: 3.597 ± 0.043	1.489GluPhe: 1.489 ± 0.021	4.489GluGly: 4.489 ± 0.041	1.524GluHis: 1.524 ± 0.024	2.234GluIle: 2.234 ± 0.033	1.459GluLys: 1.459 ± 0.028	6.501GluLeu: 6.501 ± 0.061	0.87GluMet: 0.87 ± 0.02	1.055GluAsn: 1.055 ± 0.019	3.438GluPro: 3.438 ± 0.04	2.194GluGln: 2.194 ± 0.031	5.526GluArg: 5.526 ± 0.053	2.539GluSer: 2.539 ± 0.033	2.957GluThr: 2.957 ± 0.036	4.416GluVal: 4.416 ± 0.044	0.79GluTrp: 0.79 ± 0.018	1.143GluTyr: 1.143 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	3.591PheAla: 3.591 ± 0.034	0.255PheCys: 0.255 ± 0.009	2.019PheAsp: 2.019 ± 0.03	1.434PheGlu: 1.434 ± 0.025	0.852PhePhe: 0.852 ± 0.018	2.99PheGly: 2.99 ± 0.034	0.638PheHis: 0.638 ± 0.017	0.693PheIle: 0.693 ± 0.018	0.536PheLys: 0.536 ± 0.016	2.564PheLeu: 2.564 ± 0.035	0.411PheMet: 0.411 ± 0.012	0.573PheAsn: 0.573 ± 0.016	1.391PhePro: 1.391 ± 0.024	0.71PheGln: 0.71 ± 0.015	1.85PheArg: 1.85 ± 0.025	1.446PheSer: 1.446 ± 0.022	2.076PheThr: 2.076 ± 0.024	2.235PheVal: 2.235 ± 0.032	0.436PheTrp: 0.436 ± 0.013	0.595PheTyr: 0.595 ± 0.014	0.0PheXaa: 0.0 ± 0.0
Gly	11.103GlyAla: 11.103 ± 0.072	0.825GlyCys: 0.825 ± 0.017	5.453GlyAsp: 5.453 ± 0.045	5.105GlyGlu: 5.105 ± 0.05	2.862GlyPhe: 2.862 ± 0.034	9.45GlyGly: 9.45 ± 0.125	2.318GlyHis: 2.318 ± 0.029	3.267GlyIle: 3.267 ± 0.035	2.445GlyLys: 2.445 ± 0.04	9.234GlyLeu: 9.234 ± 0.061	1.972GlyMet: 1.972 ± 0.026	1.809GlyAsn: 1.809 ± 0.028	5.227GlyPro: 5.227 ± 0.057	2.606GlyGln: 2.606 ± 0.039	7.889GlyArg: 7.889 ± 0.059	5.38GlySer: 5.38 ± 0.052	6.713GlyThr: 6.713 ± 0.062	7.619GlyVal: 7.619 ± 0.054	1.712GlyTrp: 1.712 ± 0.028	2.255GlyTyr: 2.255 ± 0.03	0.0GlyXaa: 0.0 ± 0.0
His	2.676HisAla: 2.676 ± 0.033	0.214HisCys: 0.214 ± 0.009	1.407HisAsp: 1.407 ± 0.02	1.229HisGlu: 1.229 ± 0.022	0.645HisPhe: 0.645 ± 0.016	2.44HisGly: 2.44 ± 0.028	0.712HisHis: 0.712 ± 0.018	0.706HisIle: 0.706 ± 0.017	0.377HisLys: 0.377 ± 0.013	2.472HisLeu: 2.472 ± 0.032	0.332HisMet: 0.332 ± 0.011	0.389HisAsn: 0.389 ± 0.011	1.882HisPro: 1.882 ± 0.028	0.652HisGln: 0.652 ± 0.014	2.142HisArg: 2.142 ± 0.028	1.0HisSer: 1.0 ± 0.02	1.43HisThr: 1.43 ± 0.022	1.689HisVal: 1.689 ± 0.022	0.386HisTrp: 0.386 ± 0.012	0.499HisTyr: 0.499 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	4.59IleAla: 4.59 ± 0.049	0.285IleCys: 0.285 ± 0.009	2.232IleAsp: 2.232 ± 0.032	1.907IleGlu: 1.907 ± 0.026	0.678IlePhe: 0.678 ± 0.015	3.418IleGly: 3.418 ± 0.036	0.641IleHis: 0.641 ± 0.016	0.861IleIle: 0.861 ± 0.019	0.755IleLys: 0.755 ± 0.019	2.359IleLeu: 2.359 ± 0.029	0.454IleMet: 0.454 ± 0.012	0.705IleAsn: 0.705 ± 0.018	1.732IlePro: 1.732 ± 0.026	0.706IleGln: 0.706 ± 0.018	2.231IleArg: 2.231 ± 0.025	1.591IleSer: 1.591 ± 0.026	2.263IleThr: 2.263 ± 0.029	2.674IleVal: 2.674 ± 0.034	0.389IleTrp: 0.389 ± 0.012	0.535IleTyr: 0.535 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	2.938LysAla: 2.938 ± 0.04	0.126LysCys: 0.126 ± 0.008	1.409LysAsp: 1.409 ± 0.03	1.238LysGlu: 1.238 ± 0.023	0.466LysPhe: 0.466 ± 0.014	1.981LysGly: 1.981 ± 0.033	0.433LysHis: 0.433 ± 0.013	0.859LysIle: 0.859 ± 0.021	0.928LysLys: 0.928 ± 0.026	2.036LysLeu: 2.036 ± 0.033	0.37LysMet: 0.37 ± 0.014	0.568LysAsn: 0.568 ± 0.018	1.342LysPro: 1.342 ± 0.025	0.705LysGln: 0.705 ± 0.017	1.397LysArg: 1.397 ± 0.027	1.176LysSer: 1.176 ± 0.025	1.266LysThr: 1.266 ± 0.024	1.97LysVal: 1.97 ± 0.031	0.302LysTrp: 0.302 ± 0.011	0.48LysTyr: 0.48 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	14.597LeuAla: 14.597 ± 0.099	0.867LeuCys: 0.867 ± 0.018	6.865LeuAsp: 6.865 ± 0.063	4.567LeuGlu: 4.567 ± 0.047	2.547LeuPhe: 2.547 ± 0.035	9.209LeuGly: 9.209 ± 0.061	2.295LeuHis: 2.295 ± 0.036	3.176LeuIle: 3.176 ± 0.036	2.124LeuLys: 2.124 ± 0.034	11.05LeuLeu: 11.05 ± 0.101	1.6LeuMet: 1.6 ± 0.024	1.701LeuAsn: 1.701 ± 0.026	6.496LeuPro: 6.496 ± 0.054	2.115LeuGln: 2.115 ± 0.027	8.62LeuArg: 8.62 ± 0.067	5.294LeuSer: 5.294 ± 0.04	7.169LeuThr: 7.169 ± 0.052	8.715LeuVal: 8.715 ± 0.067	1.391LeuTrp: 1.391 ± 0.026	1.912LeuTyr: 1.912 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	2.206MetAla: 2.206 ± 0.029	0.128MetCys: 0.128 ± 0.006	0.895MetAsp: 0.895 ± 0.017	0.752MetGlu: 0.752 ± 0.018	0.427MetPhe: 0.427 ± 0.013	1.31MetGly: 1.31 ± 0.024	0.347MetHis: 0.347 ± 0.011	0.628MetIle: 0.628 ± 0.015	0.419MetLys: 0.419 ± 0.014	1.577MetLeu: 1.577 ± 0.028	0.277MetMet: 0.277 ± 0.011	0.437MetAsn: 0.437 ± 0.013	1.099MetPro: 1.099 ± 0.02	0.409MetGln: 0.409 ± 0.013	1.403MetArg: 1.403 ± 0.021	1.281MetSer: 1.281 ± 0.022	1.502MetThr: 1.502 ± 0.021	1.259MetVal: 1.259 ± 0.019	0.209MetTrp: 0.209 ± 0.008	0.322MetTyr: 0.322 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.221AsnAla: 2.221 ± 0.03	0.167AsnCys: 0.167 ± 0.009	1.034AsnAsp: 1.034 ± 0.018	0.855AsnGlu: 0.855 ± 0.016	0.501AsnPhe: 0.501 ± 0.014	1.99AsnGly: 1.99 ± 0.034	0.411AsnHis: 0.411 ± 0.012	0.675AsnIle: 0.675 ± 0.015	0.433AsnLys: 0.433 ± 0.018	1.672AsnLeu: 1.672 ± 0.025	0.295AsnMet: 0.295 ± 0.011	0.47AsnAsn: 0.47 ± 0.015	1.372AsnPro: 1.372 ± 0.026	0.552AsnGln: 0.552 ± 0.017	1.316AsnArg: 1.316 ± 0.025	0.975AsnSer: 0.975 ± 0.021	1.161AsnThr: 1.161 ± 0.025	1.401AsnVal: 1.401 ± 0.025	0.321AsnTrp: 0.321 ± 0.011	0.442AsnTyr: 0.442 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	8.357ProAla: 8.357 ± 0.059	0.323ProCys: 0.323 ± 0.01	4.684ProAsp: 4.684 ± 0.045	4.395ProGlu: 4.395 ± 0.042	1.571ProPhe: 1.571 ± 0.023	6.668ProGly: 6.668 ± 0.061	1.479ProHis: 1.479 ± 0.025	1.327ProIle: 1.327 ± 0.024	1.185ProLys: 1.185 ± 0.02	5.412ProLeu: 5.412 ± 0.053	0.987ProMet: 0.987 ± 0.02	0.941ProAsn: 0.941 ± 0.018	3.717ProPro: 3.717 ± 0.068	1.657ProGln: 1.657 ± 0.027	4.01ProArg: 4.01 ± 0.037	3.37ProSer: 3.37 ± 0.037	3.48ProThr: 3.48 ± 0.038	5.484ProVal: 5.484 ± 0.047	0.945ProTrp: 0.945 ± 0.017	1.454ProTyr: 1.454 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	3.457GlnAla: 3.457 ± 0.04	0.178GlnCys: 0.178 ± 0.008	1.384GlnAsp: 1.384 ± 0.024	1.403GlnGlu: 1.403 ± 0.022	0.673GlnPhe: 0.673 ± 0.015	2.254GlnGly: 2.254 ± 0.03	0.603GlnHis: 0.603 ± 0.014	1.031GlnIle: 1.031 ± 0.02	0.621GlnLys: 0.621 ± 0.018	2.939GlnLeu: 2.939 ± 0.034	0.469GlnMet: 0.469 ± 0.014	0.538GlnAsn: 0.538 ± 0.016	1.635GlnPro: 1.635 ± 0.036	1.203GlnGln: 1.203 ± 0.026	2.301GlnArg: 2.301 ± 0.026	1.264GlnSer: 1.264 ± 0.022	1.298GlnThr: 1.298 ± 0.024	2.292GlnVal: 2.292 ± 0.032	0.502GlnTrp: 0.502 ± 0.015	0.64GlnTyr: 0.64 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	9.862ArgAla: 9.862 ± 0.071	0.565ArgCys: 0.565 ± 0.013	4.376ArgAsp: 4.376 ± 0.041	4.729ArgGlu: 4.729 ± 0.043	2.341ArgPhe: 2.341 ± 0.029	5.82ArgGly: 5.82 ± 0.049	2.2ArgHis: 2.2 ± 0.031	3.086ArgIle: 3.086 ± 0.034	1.642ArgLys: 1.642 ± 0.033	8.949ArgLeu: 8.949 ± 0.079	1.669ArgMet: 1.669 ± 0.025	1.356ArgAsn: 1.356 ± 0.021	5.098ArgPro: 5.098 ± 0.057	2.244ArgGln: 2.244 ± 0.03	7.669ArgArg: 7.669 ± 0.077	4.022ArgSer: 4.022 ± 0.042	5.532ArgThr: 5.532 ± 0.041	5.98ArgVal: 5.98 ± 0.051	1.373ArgTrp: 1.373 ± 0.025	1.801ArgTyr: 1.801 ± 0.026	0.0ArgXaa: 0.0 ± 0.0
Ser	6.649SerAla: 6.649 ± 0.051	0.415SerCys: 0.415 ± 0.013	2.774SerAsp: 2.774 ± 0.038	2.387SerGlu: 2.387 ± 0.03	1.534SerPhe: 1.534 ± 0.025	6.108SerGly: 6.108 ± 0.063	1.052SerHis: 1.052 ± 0.016	1.358SerIle: 1.358 ± 0.023	1.058SerLys: 1.058 ± 0.025	4.808SerLeu: 4.808 ± 0.044	1.037SerMet: 1.037 ± 0.02	0.916SerAsn: 0.916 ± 0.022	3.209SerPro: 3.209 ± 0.037	1.194SerGln: 1.194 ± 0.022	3.7SerArg: 3.7 ± 0.034	2.939SerSer: 2.939 ± 0.049	3.263SerThr: 3.263 ± 0.038	4.22SerVal: 4.22 ± 0.037	0.927SerTrp: 0.927 ± 0.019	1.233SerTyr: 1.233 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	9.223ThrAla: 9.223 ± 0.07	0.431ThrCys: 0.431 ± 0.012	3.826ThrAsp: 3.826 ± 0.039	3.323ThrGlu: 3.323 ± 0.04	1.71ThrPhe: 1.71 ± 0.021	6.908ThrGly: 6.908 ± 0.064	1.269ThrHis: 1.269 ± 0.021	1.724ThrIle: 1.724 ± 0.028	1.237ThrLys: 1.237 ± 0.028	5.878ThrLeu: 5.878 ± 0.045	0.932ThrMet: 0.932 ± 0.017	1.087ThrAsn: 1.087 ± 0.024	4.386ThrPro: 4.386 ± 0.041	1.331ThrGln: 1.331 ± 0.022	4.034ThrArg: 4.034 ± 0.036	3.345ThrSer: 3.345 ± 0.037	4.247ThrThr: 4.247 ± 0.042	6.257ThrVal: 6.257 ± 0.055	0.982ThrTrp: 0.982 ± 0.019	1.449ThrTyr: 1.449 ± 0.027	0.0ThrXaa: 0.0 ± 0.0
Val	10.553ValAla: 10.553 ± 0.065	0.735ValCys: 0.735 ± 0.017	5.166ValAsp: 5.166 ± 0.049	4.798ValGlu: 4.798 ± 0.045	2.415ValPhe: 2.415 ± 0.03	6.559ValGly: 6.559 ± 0.054	1.965ValHis: 1.965 ± 0.025	2.763ValIle: 2.763 ± 0.034	1.763ValLys: 1.763 ± 0.03	9.444ValLeu: 9.444 ± 0.064	1.393ValMet: 1.393 ± 0.021	1.692ValAsn: 1.692 ± 0.027	5.256ValPro: 5.256 ± 0.046	1.978ValGln: 1.978 ± 0.026	7.183ValArg: 7.183 ± 0.054	4.281ValSer: 4.281 ± 0.04	5.696ValThr: 5.696 ± 0.052	7.904ValVal: 7.904 ± 0.072	1.233ValTrp: 1.233 ± 0.02	1.608ValTyr: 1.608 ± 0.025	0.0ValXaa: 0.0 ± 0.0
Trp	1.74TrpAla: 1.74 ± 0.028	0.158TrpCys: 0.158 ± 0.008	0.898TrpAsp: 0.898 ± 0.018	0.752TrpGlu: 0.752 ± 0.016	0.523TrpPhe: 0.523 ± 0.013	1.128TrpGly: 1.128 ± 0.023	0.377TrpHis: 0.377 ± 0.011	0.583TrpIle: 0.583 ± 0.015	0.405TrpLys: 0.405 ± 0.013	1.809TrpLeu: 1.809 ± 0.03	0.28TrpMet: 0.28 ± 0.01	0.455TrpAsn: 0.455 ± 0.014	0.801TrpPro: 0.801 ± 0.016	0.608TrpGln: 0.608 ± 0.015	1.379TrpArg: 1.379 ± 0.024	0.989TrpSer: 0.989 ± 0.018	1.111TrpThr: 1.111 ± 0.023	0.998TrpVal: 0.998 ± 0.02	0.344TrpTrp: 0.344 ± 0.013	0.394TrpTyr: 0.394 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.794TyrAla: 2.794 ± 0.031	0.176TyrCys: 0.176 ± 0.008	1.583TyrAsp: 1.583 ± 0.028	1.31TyrGlu: 1.31 ± 0.024	0.646TyrPhe: 0.646 ± 0.015	2.323TyrGly: 2.323 ± 0.033	0.384TyrHis: 0.384 ± 0.011	0.532TyrIle: 0.532 ± 0.015	0.437TyrLys: 0.437 ± 0.013	2.096TyrLeu: 2.096 ± 0.027	0.261TyrMet: 0.261 ± 0.01	0.451TyrAsn: 0.451 ± 0.012	1.079TyrPro: 1.079 ± 0.017	0.597TyrGln: 0.597 ± 0.014	1.832TyrArg: 1.832 ± 0.026	0.969TyrSer: 0.969 ± 0.017	1.257TyrThr: 1.257 ± 0.024	1.773TyrVal: 1.773 ± 0.023	0.395TyrTrp: 0.395 ± 0.012	0.487TyrTyr: 0.487 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8845 proteins (2953601 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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