Paenibacillus contaminans
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8159 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A329MV39|A0A329MV39_9BACL FAA hydrolase family protein OS=Paenibacillus contaminans OX=450362 GN=DQG23_07095 PE=4 SV=1
MM1 pKa = 7.23 GVSVYY6 pKa = 7.11 YY7 pKa = 10.38 TCMRR11 pKa = 11.84 NNNLTDD17 pKa = 3.66 SEE19 pKa = 4.37 EE20 pKa = 4.27 QEE22 pKa = 3.58 ITAIIDD28 pKa = 3.26 KK29 pKa = 10.3 YY30 pKa = 10.94 NAGFEE35 pKa = 4.17 MKK37 pKa = 10.46 DD38 pKa = 2.96 IGEE41 pKa = 4.43 TFCVYY46 pKa = 10.44 DD47 pKa = 3.89 YY48 pKa = 11.48 DD49 pKa = 4.02 QDD51 pKa = 3.7 EE52 pKa = 4.5 PTVIFAGSTKK62 pKa = 10.65 LPISDD67 pKa = 4.81 DD68 pKa = 4.43 FEE70 pKa = 4.73 DD71 pKa = 3.62 TLQALFYY78 pKa = 10.29 WLACLTDD85 pKa = 3.19 IRR87 pKa = 11.84 RR88 pKa = 11.84 SISNGDD94 pKa = 3.19 WHH96 pKa = 6.73 VHH98 pKa = 6.35 LDD100 pKa = 3.66 DD101 pKa = 5.15 TDD103 pKa = 5.44 AIWDD107 pKa = 4.33 EE108 pKa = 4.19 EE109 pKa = 4.78 TGWQMPEE116 pKa = 3.92 DD117 pKa = 3.74
Molecular weight: 13.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.719
IPC_protein 3.706
Toseland 3.49
ProMoST 3.834
Dawson 3.706
Bjellqvist 3.897
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.401
Solomon 3.681
Lehninger 3.643
Nozaki 3.821
DTASelect 4.05
Thurlkill 3.554
EMBOSS 3.656
Sillero 3.834
Patrickios 0.655
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.751
Protein with the highest isoelectric point:
>tr|A0A329MJT7|A0A329MJT7_9BACL Ca(2+)/H(+) antiporter OS=Paenibacillus contaminans OX=450362 GN=cax PE=3 SV=1
MM1 pKa = 7.73 PVPAPPPRR9 pKa = 11.84 RR10 pKa = 11.84 LPPPAAPALAPPLLPRR26 pKa = 11.84 RR27 pKa = 11.84 FPRR30 pKa = 11.84 SPPALAPSS38 pKa = 3.62
Molecular weight: 3.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.386
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.901
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.16
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8159
0
8159
2897758
16
4862
355.2
39.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.619 ± 0.032
0.776 ± 0.008
5.252 ± 0.018
6.553 ± 0.029
4.311 ± 0.017
7.58 ± 0.028
1.984 ± 0.013
6.381 ± 0.024
5.336 ± 0.026
9.768 ± 0.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.764 ± 0.012
3.908 ± 0.02
4.199 ± 0.018
3.622 ± 0.015
5.157 ± 0.027
6.336 ± 0.021
5.433 ± 0.03
6.99 ± 0.023
1.384 ± 0.009
3.644 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here