Amino acid dipepetide frequency for Colwellia sp. RSH04

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.093AlaAla: 6.093 ± 0.101
0.93AlaCys: 0.93 ± 0.031
4.242AlaAsp: 4.242 ± 0.067
4.895AlaGlu: 4.895 ± 0.075
3.386AlaPhe: 3.386 ± 0.052
5.039AlaGly: 5.039 ± 0.077
1.552AlaHis: 1.552 ± 0.034
6.436AlaIle: 6.436 ± 0.071
5.695AlaLys: 5.695 ± 0.088
8.927AlaLeu: 8.927 ± 0.092
2.208AlaMet: 2.208 ± 0.048
4.23AlaAsn: 4.23 ± 0.059
2.419AlaPro: 2.419 ± 0.051
3.843AlaGln: 3.843 ± 0.059
2.84AlaArg: 2.84 ± 0.057
5.329AlaSer: 5.329 ± 0.069
4.131AlaThr: 4.131 ± 0.065
4.968AlaVal: 4.968 ± 0.066
0.823AlaTrp: 0.823 ± 0.028
2.304AlaTyr: 2.304 ± 0.044
0.0AlaXaa: 0.0 ± 0.0
Cys
0.715CysAla: 0.715 ± 0.027
0.153CysCys: 0.153 ± 0.012
0.556CysAsp: 0.556 ± 0.022
0.553CysGlu: 0.553 ± 0.023
0.498CysPhe: 0.498 ± 0.02
0.775CysGly: 0.775 ± 0.031
0.336CysHis: 0.336 ± 0.018
0.669CysIle: 0.669 ± 0.026
0.487CysLys: 0.487 ± 0.022
0.997CysLeu: 0.997 ± 0.029
0.192CysMet: 0.192 ± 0.013
0.423CysAsn: 0.423 ± 0.02
0.443CysPro: 0.443 ± 0.021
0.575CysGln: 0.575 ± 0.024
0.366CysArg: 0.366 ± 0.017
0.775CysSer: 0.775 ± 0.026
0.476CysThr: 0.476 ± 0.022
0.56CysVal: 0.56 ± 0.022
0.125CysTrp: 0.125 ± 0.01
0.336CysTyr: 0.336 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
3.928AspAla: 3.928 ± 0.068
0.537AspCys: 0.537 ± 0.022
3.27AspAsp: 3.27 ± 0.091
4.012AspGlu: 4.012 ± 0.057
2.613AspPhe: 2.613 ± 0.052
3.459AspGly: 3.459 ± 0.103
0.98AspHis: 0.98 ± 0.034
4.793AspIle: 4.793 ± 0.073
3.849AspLys: 3.849 ± 0.066
4.94AspLeu: 4.94 ± 0.068
1.291AspMet: 1.291 ± 0.035
3.198AspAsn: 3.198 ± 0.063
1.59AspPro: 1.59 ± 0.042
1.62AspGln: 1.62 ± 0.038
1.851AspArg: 1.851 ± 0.046
3.493AspSer: 3.493 ± 0.061
2.622AspThr: 2.622 ± 0.053
3.775AspVal: 3.775 ± 0.066
0.73AspTrp: 0.73 ± 0.022
2.166AspTyr: 2.166 ± 0.049
0.0AspXaa: 0.0 ± 0.0
Glu
4.485GluAla: 4.485 ± 0.065
0.468GluCys: 0.468 ± 0.023
2.838GluAsp: 2.838 ± 0.047
3.824GluGlu: 3.824 ± 0.077
2.53GluPhe: 2.53 ± 0.045
3.166GluGly: 3.166 ± 0.057
1.746GluHis: 1.746 ± 0.045
4.107GluIle: 4.107 ± 0.062
4.543GluLys: 4.543 ± 0.081
6.96GluLeu: 6.96 ± 0.088
1.515GluMet: 1.515 ± 0.033
3.336GluAsn: 3.336 ± 0.05
1.743GluPro: 1.743 ± 0.041
4.762GluGln: 4.762 ± 0.07
2.643GluArg: 2.643 ± 0.055
3.892GluSer: 3.892 ± 0.058
3.066GluThr: 3.066 ± 0.061
4.193GluVal: 4.193 ± 0.068
0.561GluTrp: 0.561 ± 0.023
1.858GluTyr: 1.858 ± 0.045
0.0GluXaa: 0.0 ± 0.0
Phe
3.527PheAla: 3.527 ± 0.065
0.487PheCys: 0.487 ± 0.02
2.898PheAsp: 2.898 ± 0.052
2.659PheGlu: 2.659 ± 0.049
1.932PhePhe: 1.932 ± 0.044
2.885PheGly: 2.885 ± 0.06
0.823PheHis: 0.823 ± 0.028
3.567PheIle: 3.567 ± 0.059
2.427PheLys: 2.427 ± 0.053
3.585PheLeu: 3.585 ± 0.065
0.963PheMet: 0.963 ± 0.031
2.568PheAsn: 2.568 ± 0.046
1.333PhePro: 1.333 ± 0.038
1.276PheGln: 1.276 ± 0.033
1.307PheArg: 1.307 ± 0.035
3.933PheSer: 3.933 ± 0.066
2.72PheThr: 2.72 ± 0.05
2.633PheVal: 2.633 ± 0.046
0.516PheTrp: 0.516 ± 0.024
1.576PheTyr: 1.576 ± 0.038
0.0PheXaa: 0.0 ± 0.0
Gly
4.489GlyAla: 4.489 ± 0.08
0.743GlyCys: 0.743 ± 0.027
3.515GlyAsp: 3.515 ± 0.076
4.075GlyGlu: 4.075 ± 0.068
3.213GlyPhe: 3.213 ± 0.054
4.23GlyGly: 4.23 ± 0.087
1.363GlyHis: 1.363 ± 0.039
4.566GlyIle: 4.566 ± 0.065
4.089GlyLys: 4.089 ± 0.063
6.049GlyLeu: 6.049 ± 0.077
1.678GlyMet: 1.678 ± 0.038
2.888GlyAsn: 2.888 ± 0.069
1.343GlyPro: 1.343 ± 0.038
2.569GlyGln: 2.569 ± 0.05
2.426GlyArg: 2.426 ± 0.046
3.839GlySer: 3.839 ± 0.079
3.203GlyThr: 3.203 ± 0.065
4.553GlyVal: 4.553 ± 0.075
0.806GlyTrp: 0.806 ± 0.026
2.458GlyTyr: 2.458 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
1.449HisAla: 1.449 ± 0.034
0.347HisCys: 0.347 ± 0.017
1.104HisAsp: 1.104 ± 0.03
1.181HisGlu: 1.181 ± 0.037
1.145HisPhe: 1.145 ± 0.034
1.466HisGly: 1.466 ± 0.034
0.699HisHis: 0.699 ± 0.024
1.53HisIle: 1.53 ± 0.04
1.218HisLys: 1.218 ± 0.036
2.188HisLeu: 2.188 ± 0.052
0.435HisMet: 0.435 ± 0.018
1.099HisAsn: 1.099 ± 0.029
0.921HisPro: 0.921 ± 0.03
1.31HisGln: 1.31 ± 0.036
0.823HisArg: 0.823 ± 0.022
1.437HisSer: 1.437 ± 0.036
1.006HisThr: 1.006 ± 0.027
1.277HisVal: 1.277 ± 0.031
0.376HisTrp: 0.376 ± 0.017
0.927HisTyr: 0.927 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.685IleAla: 6.685 ± 0.091
0.706IleCys: 0.706 ± 0.023
4.668IleAsp: 4.668 ± 0.066
5.292IleGlu: 5.292 ± 0.084
2.72IlePhe: 2.72 ± 0.058
4.423IleGly: 4.423 ± 0.069
1.293IleHis: 1.293 ± 0.033
4.784IleIle: 4.784 ± 0.069
4.65IleLys: 4.65 ± 0.07
5.69IleLeu: 5.69 ± 0.077
1.322IleMet: 1.322 ± 0.036
4.421IleAsn: 4.421 ± 0.069
2.591IlePro: 2.591 ± 0.044
2.287IleGln: 2.287 ± 0.049
2.469IleArg: 2.469 ± 0.047
5.691IleSer: 5.691 ± 0.076
4.533IleThr: 4.533 ± 0.066
4.238IleVal: 4.238 ± 0.073
0.607IleTrp: 0.607 ± 0.024
1.968IleTyr: 1.968 ± 0.042
0.0IleXaa: 0.0 ± 0.0
Lys
5.5LysAla: 5.5 ± 0.087
0.35LysCys: 0.35 ± 0.024
3.088LysAsp: 3.088 ± 0.058
4.229LysGlu: 4.229 ± 0.073
1.953LysPhe: 1.953 ± 0.039
3.45LysGly: 3.45 ± 0.058
1.595LysHis: 1.595 ± 0.036
3.838LysIle: 3.838 ± 0.059
4.175LysLys: 4.175 ± 0.072
6.216LysLeu: 6.216 ± 0.077
1.517LysMet: 1.517 ± 0.037
3.127LysAsn: 3.127 ± 0.054
2.492LysPro: 2.492 ± 0.048
3.716LysGln: 3.716 ± 0.066
2.722LysArg: 2.722 ± 0.053
4.142LysSer: 4.142 ± 0.068
3.542LysThr: 3.542 ± 0.051
4.418LysVal: 4.418 ± 0.065
0.552LysTrp: 0.552 ± 0.026
1.878LysTyr: 1.878 ± 0.04
0.0LysXaa: 0.0 ± 0.0
Leu
9.654LeuAla: 9.654 ± 0.097
0.996LeuCys: 0.996 ± 0.029
5.299LeuAsp: 5.299 ± 0.086
5.413LeuGlu: 5.413 ± 0.082
4.421LeuPhe: 4.421 ± 0.086
6.053LeuGly: 6.053 ± 0.078
1.924LeuHis: 1.924 ± 0.04
6.878LeuIle: 6.878 ± 0.099
5.877LeuLys: 5.877 ± 0.075
10.632LeuLeu: 10.632 ± 0.141
2.469LeuMet: 2.469 ± 0.045
5.259LeuAsn: 5.259 ± 0.072
4.41LeuPro: 4.41 ± 0.06
3.589LeuGln: 3.589 ± 0.058
3.479LeuArg: 3.479 ± 0.057
8.73LeuSer: 8.73 ± 0.089
6.738LeuThr: 6.738 ± 0.085
6.414LeuVal: 6.414 ± 0.085
1.023LeuTrp: 1.023 ± 0.032
2.74LeuTyr: 2.74 ± 0.055
0.0LeuXaa: 0.0 ± 0.0
Met
2.137MetAla: 2.137 ± 0.042
0.211MetCys: 0.211 ± 0.014
1.022MetAsp: 1.022 ± 0.03
1.104MetGlu: 1.104 ± 0.035
0.843MetPhe: 0.843 ± 0.031
1.426MetGly: 1.426 ± 0.039
0.475MetHis: 0.475 ± 0.019
1.437MetIle: 1.437 ± 0.036
1.534MetLys: 1.534 ± 0.036
2.503MetLeu: 2.503 ± 0.052
0.685MetMet: 0.685 ± 0.025
1.174MetAsn: 1.174 ± 0.032
1.132MetPro: 1.132 ± 0.033
1.025MetGln: 1.025 ± 0.03
0.941MetArg: 0.941 ± 0.026
1.927MetSer: 1.927 ± 0.04
1.514MetThr: 1.514 ± 0.035
1.489MetVal: 1.489 ± 0.037
0.206MetTrp: 0.206 ± 0.014
0.566MetTyr: 0.566 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
3.877AsnAla: 3.877 ± 0.067
0.487AsnCys: 0.487 ± 0.02
3.08AsnAsp: 3.08 ± 0.058
3.378AsnGlu: 3.378 ± 0.056
2.064AsnPhe: 2.064 ± 0.043
3.129AsnGly: 3.129 ± 0.063
1.136AsnHis: 1.136 ± 0.031
3.964AsnIle: 3.964 ± 0.062
3.706AsnLys: 3.706 ± 0.057
4.538AsnLeu: 4.538 ± 0.061
1.072AsnMet: 1.072 ± 0.032
3.148AsnAsn: 3.148 ± 0.061
1.913AsnPro: 1.913 ± 0.045
2.472AsnGln: 2.472 ± 0.059
1.899AsnArg: 1.899 ± 0.043
3.441AsnSer: 3.441 ± 0.067
3.047AsnThr: 3.047 ± 0.064
3.088AsnVal: 3.088 ± 0.055
0.626AsnTrp: 0.626 ± 0.024
1.763AsnTyr: 1.763 ± 0.038
0.0AsnXaa: 0.0 ± 0.0
Pro
2.541ProAla: 2.541 ± 0.05
0.289ProCys: 0.289 ± 0.015
1.963ProAsp: 1.963 ± 0.039
2.869ProGlu: 2.869 ± 0.058
1.588ProPhe: 1.588 ± 0.035
1.825ProGly: 1.825 ± 0.048
0.721ProHis: 0.721 ± 0.026
2.462ProIle: 2.462 ± 0.046
2.115ProLys: 2.115 ± 0.045
3.757ProLeu: 3.757 ± 0.061
0.876ProMet: 0.876 ± 0.03
1.703ProAsn: 1.703 ± 0.041
0.915ProPro: 0.915 ± 0.029
1.39ProGln: 1.39 ± 0.038
1.12ProArg: 1.12 ± 0.03
2.395ProSer: 2.395 ± 0.042
1.917ProThr: 1.917 ± 0.043
2.584ProVal: 2.584 ± 0.045
0.466ProTrp: 0.466 ± 0.021
1.222ProTyr: 1.222 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
4.298GlnAla: 4.298 ± 0.067
0.468GlnCys: 0.468 ± 0.023
2.007GlnAsp: 2.007 ± 0.042
2.666GlnGlu: 2.666 ± 0.054
1.849GlnPhe: 1.849 ± 0.044
3.18GlnGly: 3.18 ± 0.055
1.28GlnHis: 1.28 ± 0.031
2.567GlnIle: 2.567 ± 0.048
2.475GlnLys: 2.475 ± 0.047
5.502GlnLeu: 5.502 ± 0.085
0.987GlnMet: 0.987 ± 0.029
1.867GlnAsn: 1.867 ± 0.037
1.498GlnPro: 1.498 ± 0.038
3.402GlnGln: 3.402 ± 0.094
1.967GlnArg: 1.967 ± 0.04
3.058GlnSer: 3.058 ± 0.058
2.199GlnThr: 2.199 ± 0.042
3.37GlnVal: 3.37 ± 0.057
0.65GlnTrp: 0.65 ± 0.024
1.558GlnTyr: 1.558 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
2.806ArgAla: 2.806 ± 0.046
0.392ArgCys: 0.392 ± 0.018
1.979ArgAsp: 1.979 ± 0.043
2.323ArgGlu: 2.323 ± 0.048
1.901ArgPhe: 1.901 ± 0.039
2.098ArgGly: 2.098 ± 0.044
0.86ArgHis: 0.86 ± 0.029
2.676ArgIle: 2.676 ± 0.056
2.279ArgLys: 2.279 ± 0.041
4.106ArgLeu: 4.106 ± 0.064
0.905ArgMet: 0.905 ± 0.027
1.801ArgAsn: 1.801 ± 0.047
1.191ArgPro: 1.191 ± 0.035
1.696ArgGln: 1.696 ± 0.043
1.691ArgArg: 1.691 ± 0.036
2.17ArgSer: 2.17 ± 0.042
1.802ArgThr: 1.802 ± 0.038
2.582ArgVal: 2.582 ± 0.054
0.478ArgTrp: 0.478 ± 0.02
1.507ArgTyr: 1.507 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
5.429SerAla: 5.429 ± 0.074
0.711SerCys: 0.711 ± 0.027
3.936SerAsp: 3.936 ± 0.067
4.258SerGlu: 4.258 ± 0.062
3.504SerPhe: 3.504 ± 0.067
4.857SerGly: 4.857 ± 0.074
1.596SerHis: 1.596 ± 0.038
4.95SerIle: 4.95 ± 0.066
4.187SerLys: 4.187 ± 0.07
7.634SerLeu: 7.634 ± 0.089
1.639SerMet: 1.639 ± 0.04
3.459SerAsn: 3.459 ± 0.06
2.359SerPro: 2.359 ± 0.05
3.33SerGln: 3.33 ± 0.057
2.501SerArg: 2.501 ± 0.053
5.093SerSer: 5.093 ± 0.084
3.838SerThr: 3.838 ± 0.064
4.634SerVal: 4.634 ± 0.076
0.818SerTrp: 0.818 ± 0.03
2.414SerTyr: 2.414 ± 0.052
0.0SerXaa: 0.0 ± 0.0
Thr
4.24ThrAla: 4.24 ± 0.058
0.506ThrCys: 0.506 ± 0.021
3.125ThrAsp: 3.125 ± 0.063
3.288ThrGlu: 3.288 ± 0.057
2.34ThrPhe: 2.34 ± 0.053
3.825ThrGly: 3.825 ± 0.072
1.112ThrHis: 1.112 ± 0.029
4.074ThrIle: 4.074 ± 0.066
3.023ThrLys: 3.023 ± 0.055
6.366ThrLeu: 6.366 ± 0.077
1.133ThrMet: 1.133 ± 0.03
2.574ThrAsn: 2.574 ± 0.049
2.536ThrPro: 2.536 ± 0.052
2.413ThrGln: 2.413 ± 0.047
1.907ThrArg: 1.907 ± 0.039
3.797ThrSer: 3.797 ± 0.067
3.128ThrThr: 3.128 ± 0.059
3.761ThrVal: 3.761 ± 0.068
0.573ThrTrp: 0.573 ± 0.023
1.59ThrTyr: 1.59 ± 0.042
0.0ThrXaa: 0.0 ± 0.0
Val
5.554ValAla: 5.554 ± 0.074
0.677ValCys: 0.677 ± 0.024
3.952ValAsp: 3.952 ± 0.058
4.306ValGlu: 4.306 ± 0.061
2.849ValPhe: 2.849 ± 0.055
4.035ValGly: 4.035 ± 0.07
1.211ValHis: 1.211 ± 0.033
5.136ValIle: 5.136 ± 0.065
3.998ValLys: 3.998 ± 0.06
6.225ValLeu: 6.225 ± 0.074
1.598ValMet: 1.598 ± 0.047
3.679ValAsn: 3.679 ± 0.06
2.209ValPro: 2.209 ± 0.049
2.02ValGln: 2.02 ± 0.04
2.3ValArg: 2.3 ± 0.046
4.946ValSer: 4.946 ± 0.077
3.886ValThr: 3.886 ± 0.065
4.678ValVal: 4.678 ± 0.078
0.648ValTrp: 0.648 ± 0.024
1.799ValTyr: 1.799 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
0.685TrpAla: 0.685 ± 0.024
0.131TrpCys: 0.131 ± 0.011
0.492TrpAsp: 0.492 ± 0.023
0.414TrpGlu: 0.414 ± 0.019
0.581TrpPhe: 0.581 ± 0.025
0.683TrpGly: 0.683 ± 0.03
0.374TrpHis: 0.374 ± 0.018
0.549TrpIle: 0.549 ± 0.021
0.446TrpLys: 0.446 ± 0.019
1.652TrpLeu: 1.652 ± 0.043
0.252TrpMet: 0.252 ± 0.016
0.419TrpAsn: 0.419 ± 0.021
0.4TrpPro: 0.4 ± 0.017
1.209TrpGln: 1.209 ± 0.036
0.554TrpArg: 0.554 ± 0.02
0.722TrpSer: 0.722 ± 0.028
0.414TrpThr: 0.414 ± 0.019
0.714TrpVal: 0.714 ± 0.025
0.163TrpTrp: 0.163 ± 0.012
0.373TrpTyr: 0.373 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.198TyrAla: 2.198 ± 0.039
0.423TyrCys: 0.423 ± 0.021
1.684TyrAsp: 1.684 ± 0.045
1.496TyrGlu: 1.496 ± 0.036
1.685TyrPhe: 1.685 ± 0.041
2.092TyrGly: 2.092 ± 0.045
0.864TyrHis: 0.864 ± 0.027
2.056TyrIle: 2.056 ± 0.043
1.728TyrLys: 1.728 ± 0.041
3.532TyrLeu: 3.532 ± 0.057
0.612TyrMet: 0.612 ± 0.027
1.456TyrAsn: 1.456 ± 0.038
1.28TyrPro: 1.28 ± 0.034
2.345TyrGln: 2.345 ± 0.048
1.436TyrArg: 1.436 ± 0.034
2.336TyrSer: 2.336 ± 0.056
1.53TyrThr: 1.53 ± 0.041
1.774TyrVal: 1.774 ± 0.037
0.478TyrTrp: 0.478 ± 0.02
1.17TyrTyr: 1.17 ± 0.036
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3551 proteins (1191842 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski