Gordonia phage Geodirt

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vividuovirus; unclassified Vividuovirus

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A3G3M918|A0A3G3M918_9CAUD Uncharacterized protein OS=Gordonia phage Geodirt OX=2483670 GN=11 PE=4 SV=1
MM1 pKa = 6.21TTRR4 pKa = 11.84VQSVLVEE11 pKa = 4.17VPTDD15 pKa = 3.72DD16 pKa = 3.88FTSSDD21 pKa = 3.72VQVSRR26 pKa = 11.84MVVDD30 pKa = 3.76VPVDD34 pKa = 3.4GFGPSLVEE42 pKa = 3.66SLYY45 pKa = 10.43PSTTLYY51 pKa = 10.25PSEE54 pKa = 4.76DD55 pKa = 4.25LYY57 pKa = 10.49PTGPPSS63 pKa = 3.12

Molecular weight:
6.81 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A3G3MA01|A0A3G3MA01_9CAUD Uncharacterized protein OS=Gordonia phage Geodirt OX=2483670 GN=43 PE=4 SV=1
MM1 pKa = 8.21DD2 pKa = 6.27DD3 pKa = 4.49STEE6 pKa = 3.83QWHH9 pKa = 7.14PIPGHH14 pKa = 4.91PTYY17 pKa = 10.27EE18 pKa = 3.83ASTAGRR24 pKa = 11.84VRR26 pKa = 11.84SVKK29 pKa = 9.82PGRR32 pKa = 11.84APRR35 pKa = 11.84VLKK38 pKa = 9.1PTLNEE43 pKa = 3.16RR44 pKa = 11.84GYY46 pKa = 9.39YY47 pKa = 9.17QFSTSCDD54 pKa = 2.97GKK56 pKa = 10.39RR57 pKa = 11.84RR58 pKa = 11.84QLKK61 pKa = 9.83VSVAVLLAFVGPRR74 pKa = 11.84PTRR77 pKa = 11.84RR78 pKa = 11.84HH79 pKa = 5.5HH80 pKa = 6.08CCHH83 pKa = 7.19RR84 pKa = 11.84DD85 pKa = 3.12TDD87 pKa = 3.95KK88 pKa = 11.71TNDD91 pKa = 3.36ALSNLRR97 pKa = 11.84WDD99 pKa = 4.28TPQGNYY105 pKa = 10.29LDD107 pKa = 3.99TFNAGRR113 pKa = 11.84NHH115 pKa = 6.54NSQKK119 pKa = 8.79MACPAGHH126 pKa = 6.73EE127 pKa = 4.24YY128 pKa = 10.18IPEE131 pKa = 3.77NTYY134 pKa = 10.31VNRR137 pKa = 11.84VGHH140 pKa = 5.33RR141 pKa = 11.84QCRR144 pKa = 11.84ICRR147 pKa = 11.84RR148 pKa = 11.84EE149 pKa = 3.86SEE151 pKa = 4.13RR152 pKa = 11.84RR153 pKa = 11.84RR154 pKa = 11.84APRR157 pKa = 11.84RR158 pKa = 11.84RR159 pKa = 11.84RR160 pKa = 3.33

Molecular weight:
18.56 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

0

85

18846

37

1608

221.7

24.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.278 ± 0.483

0.716 ± 0.11

7.391 ± 0.426

5.619 ± 0.401

2.478 ± 0.176

8.479 ± 0.44

2.085 ± 0.217

4.553 ± 0.258

2.584 ± 0.197

7.577 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.107 ± 0.088

2.696 ± 0.157

6.309 ± 0.277

3.858 ± 0.185

7.37 ± 0.467

5.073 ± 0.218

6.829 ± 0.337

7.689 ± 0.271

2.128 ± 0.132

2.181 ± 0.154

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski