Gordonia phage Geodirt
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G3M918|A0A3G3M918_9CAUD Uncharacterized protein OS=Gordonia phage Geodirt OX=2483670 GN=11 PE=4 SV=1
MM1 pKa = 6.21 TTRR4 pKa = 11.84 VQSVLVEE11 pKa = 4.17 VPTDD15 pKa = 3.72 DD16 pKa = 3.88 FTSSDD21 pKa = 3.72 VQVSRR26 pKa = 11.84 MVVDD30 pKa = 3.76 VPVDD34 pKa = 3.4 GFGPSLVEE42 pKa = 3.66 SLYY45 pKa = 10.43 PSTTLYY51 pKa = 10.25 PSEE54 pKa = 4.76 DD55 pKa = 4.25 LYY57 pKa = 10.49 PTGPPSS63 pKa = 3.12
Molecular weight: 6.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.828
IPC2_protein 3.656
IPC_protein 3.516
Toseland 3.338
ProMoST 3.757
Dawson 3.554
Bjellqvist 3.719
Wikipedia 3.554
Rodwell 3.376
Grimsley 3.261
Solomon 3.49
Lehninger 3.439
Nozaki 3.706
DTASelect 3.897
Thurlkill 3.439
EMBOSS 3.554
Sillero 3.656
Patrickios 1.837
IPC_peptide 3.478
IPC2_peptide 3.617
IPC2.peptide.svr19 3.691
Protein with the highest isoelectric point:
>tr|A0A3G3MA01|A0A3G3MA01_9CAUD Uncharacterized protein OS=Gordonia phage Geodirt OX=2483670 GN=43 PE=4 SV=1
MM1 pKa = 8.21 DD2 pKa = 6.27 DD3 pKa = 4.49 STEE6 pKa = 3.83 QWHH9 pKa = 7.14 PIPGHH14 pKa = 4.91 PTYY17 pKa = 10.27 EE18 pKa = 3.83 ASTAGRR24 pKa = 11.84 VRR26 pKa = 11.84 SVKK29 pKa = 9.82 PGRR32 pKa = 11.84 APRR35 pKa = 11.84 VLKK38 pKa = 9.1 PTLNEE43 pKa = 3.16 RR44 pKa = 11.84 GYY46 pKa = 9.39 YY47 pKa = 9.17 QFSTSCDD54 pKa = 2.97 GKK56 pKa = 10.39 RR57 pKa = 11.84 RR58 pKa = 11.84 QLKK61 pKa = 9.83 VSVAVLLAFVGPRR74 pKa = 11.84 PTRR77 pKa = 11.84 RR78 pKa = 11.84 HH79 pKa = 5.5 HH80 pKa = 6.08 CCHH83 pKa = 7.19 RR84 pKa = 11.84 DD85 pKa = 3.12 TDD87 pKa = 3.95 KK88 pKa = 11.71 TNDD91 pKa = 3.36 ALSNLRR97 pKa = 11.84 WDD99 pKa = 4.28 TPQGNYY105 pKa = 10.29 LDD107 pKa = 3.99 TFNAGRR113 pKa = 11.84 NHH115 pKa = 6.54 NSQKK119 pKa = 8.79 MACPAGHH126 pKa = 6.73 EE127 pKa = 4.24 YY128 pKa = 10.18 IPEE131 pKa = 3.77 NTYY134 pKa = 10.31 VNRR137 pKa = 11.84 VGHH140 pKa = 5.33 RR141 pKa = 11.84 QCRR144 pKa = 11.84 ICRR147 pKa = 11.84 RR148 pKa = 11.84 EE149 pKa = 3.86 SEE151 pKa = 4.13 RR152 pKa = 11.84 RR153 pKa = 11.84 RR154 pKa = 11.84 APRR157 pKa = 11.84 RR158 pKa = 11.84 RR159 pKa = 11.84 RR160 pKa = 3.33
Molecular weight: 18.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.604
IPC_protein 10.555
Toseland 10.467
ProMoST 10.452
Dawson 10.628
Bjellqvist 10.409
Wikipedia 10.877
Rodwell 10.687
Grimsley 10.687
Solomon 10.73
Lehninger 10.687
Nozaki 10.511
DTASelect 10.379
Thurlkill 10.511
EMBOSS 10.877
Sillero 10.57
Patrickios 10.35
IPC_peptide 10.73
IPC2_peptide 9.75
IPC2.peptide.svr19 8.547
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
85
0
85
18846
37
1608
221.7
24.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.278 ± 0.483
0.716 ± 0.11
7.391 ± 0.426
5.619 ± 0.401
2.478 ± 0.176
8.479 ± 0.44
2.085 ± 0.217
4.553 ± 0.258
2.584 ± 0.197
7.577 ± 0.273
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.107 ± 0.088
2.696 ± 0.157
6.309 ± 0.277
3.858 ± 0.185
7.37 ± 0.467
5.073 ± 0.218
6.829 ± 0.337
7.689 ± 0.271
2.128 ± 0.132
2.181 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here