Geobacillus phage GBK1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9LX04|A0A6B9LX04_9CAUD DNA primase OS=Geobacillus phage GBK1 OX=2686286 GN=GBK1_26 PE=4 SV=1
MM1 pKa = 7.7PWYY4 pKa = 10.04KK5 pKa = 10.47YY6 pKa = 10.32LRR8 pKa = 11.84AYY10 pKa = 10.03QGLGTGWISFDD21 pKa = 3.35LTNVFMNDD29 pKa = 3.62YY30 pKa = 11.26KK31 pKa = 11.07NGTYY35 pKa = 10.12HH36 pKa = 7.2GVVLYY41 pKa = 10.8SGASSGYY48 pKa = 7.97YY49 pKa = 10.22GEE51 pKa = 4.99AYY53 pKa = 10.21GYY55 pKa = 8.39RR56 pKa = 11.84TDD58 pKa = 3.81SYY60 pKa = 10.48CAYY63 pKa = 10.9VEE65 pKa = 4.51VTGSWNSPPSAPTITYY81 pKa = 7.83PQGGEE86 pKa = 3.97IVDD89 pKa = 3.48QSITVRR95 pKa = 11.84WNKK98 pKa = 10.35ASDD101 pKa = 3.9PDD103 pKa = 4.26GDD105 pKa = 3.92PLSYY109 pKa = 10.48EE110 pKa = 4.09VAINDD115 pKa = 3.68GTGWKK120 pKa = 9.84YY121 pKa = 11.34YY122 pKa = 9.09KK123 pKa = 9.6TATGATSYY131 pKa = 10.29TINTSSLRR139 pKa = 11.84EE140 pKa = 3.78TSSARR145 pKa = 11.84VAVRR149 pKa = 11.84AYY151 pKa = 10.37DD152 pKa = 3.68GQEE155 pKa = 3.95YY156 pKa = 10.97SSWTYY161 pKa = 11.56SNYY164 pKa = 9.97FAIDD168 pKa = 3.41HH169 pKa = 6.1NQPPSAPTQLSPATGAVIDD188 pKa = 3.71RR189 pKa = 11.84TQPNRR194 pKa = 11.84FSWKK198 pKa = 10.23HH199 pKa = 5.57NDD201 pKa = 3.65DD202 pKa = 3.68GAQVGFRR209 pKa = 11.84IAWRR213 pKa = 11.84TVAADD218 pKa = 3.68GTRR221 pKa = 11.84GAWNYY226 pKa = 10.0IPNATSFMNTTNQYY240 pKa = 10.85YY241 pKa = 10.9DD242 pKa = 3.59MPANTLPFGNIEE254 pKa = 4.02WGVQTKK260 pKa = 9.88DD261 pKa = 3.35QQGLQSPYY269 pKa = 10.38SQWQIIKK276 pKa = 10.48ASQPTNAPTILSPTDD291 pKa = 3.47FAEE294 pKa = 4.22INTTRR299 pKa = 11.84VTVTWSSLNQIQYY312 pKa = 11.36DD313 pKa = 3.83LALYY317 pKa = 10.62DD318 pKa = 3.54SDD320 pKa = 3.21GWEE323 pKa = 4.26LFHH326 pKa = 6.55EE327 pKa = 4.91VKK329 pKa = 10.78SGGTKK334 pKa = 9.7QVTIPVDD341 pKa = 3.54LEE343 pKa = 4.02NNKK346 pKa = 9.83FYY348 pKa = 10.53EE349 pKa = 3.63IHH351 pKa = 6.39LRR353 pKa = 11.84VMDD356 pKa = 4.0STTGLWSDD364 pKa = 3.57YY365 pKa = 9.31TVVVFSTNFTPPLPPVIEE383 pKa = 4.28RR384 pKa = 11.84FEE386 pKa = 3.98EE387 pKa = 4.67AGDD390 pKa = 3.7GVINIFYY397 pKa = 9.9GASDD401 pKa = 4.06ADD403 pKa = 3.62ILPSFLVNDD412 pKa = 4.78GQQNPLLQGYY422 pKa = 8.58SGSVIGTDD430 pKa = 4.26CEE432 pKa = 4.25LLSPSSVSITGALKK446 pKa = 10.55GVEE449 pKa = 3.88YY450 pKa = 10.17WLTNEE455 pKa = 4.96EE456 pKa = 4.16IPLVAGSVYY465 pKa = 10.58TLTATFDD472 pKa = 3.65VQGGRR477 pKa = 11.84LFIGAYY483 pKa = 9.5DD484 pKa = 3.87ANNNAISFQATGADD498 pKa = 3.53KK499 pKa = 10.97ALSPVGTTALNYY511 pKa = 7.34TLPEE515 pKa = 4.01NAVKK519 pKa = 10.54LRR521 pKa = 11.84VIFYY525 pKa = 6.97TTWDD529 pKa = 3.33NTGTVTISNANLRR542 pKa = 11.84ISNASTATEE551 pKa = 4.5KK552 pKa = 10.48IQVFRR557 pKa = 11.84RR558 pKa = 11.84EE559 pKa = 3.98YY560 pKa = 9.83TPTGTAPWIMIADD573 pKa = 4.63DD574 pKa = 4.83LPTSGSFLDD583 pKa = 3.73YY584 pKa = 10.41TPASGVIYY592 pKa = 9.52EE593 pKa = 4.29YY594 pKa = 11.29KK595 pKa = 10.49LRR597 pKa = 11.84AVNNSNKK604 pKa = 9.44TSTDD608 pKa = 2.88SSIEE612 pKa = 3.74QVSIVFNDD620 pKa = 3.57TFIQEE625 pKa = 4.31ANNLSSIVLLKK636 pKa = 10.71YY637 pKa = 8.89ATSRR641 pKa = 11.84EE642 pKa = 4.05TKK644 pKa = 9.5MEE646 pKa = 4.25LEE648 pKa = 4.2SEE650 pKa = 4.34LMRR653 pKa = 11.84FAGRR657 pKa = 11.84RR658 pKa = 11.84DD659 pKa = 3.36PVRR662 pKa = 11.84EE663 pKa = 3.98FGEE666 pKa = 4.37HH667 pKa = 5.63EE668 pKa = 4.99DD669 pKa = 3.52IVISVEE675 pKa = 4.22WIFDD679 pKa = 3.57TYY681 pKa = 11.36QEE683 pKa = 4.14VEE685 pKa = 4.17MFKK688 pKa = 11.05DD689 pKa = 3.39LLRR692 pKa = 11.84RR693 pKa = 11.84RR694 pKa = 11.84DD695 pKa = 3.42VLLYY699 pKa = 9.92RR700 pKa = 11.84DD701 pKa = 3.5RR702 pKa = 11.84NGRR705 pKa = 11.84RR706 pKa = 11.84FWVTCDD712 pKa = 3.02AFEE715 pKa = 5.15VKK717 pKa = 10.75DD718 pKa = 3.61NDD720 pKa = 3.85VNGFTLKK727 pKa = 10.76ADD729 pKa = 3.41FHH731 pKa = 5.67VTHH734 pKa = 7.34FIEE737 pKa = 5.41DD738 pKa = 3.64LAVRR742 pKa = 11.84NGEE745 pKa = 4.13EE746 pKa = 3.88TT747 pKa = 3.31

Molecular weight: 83.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9LPB7|A0A6B9LPB7_9CAUD Uncharacterized protein OS=Geobacillus phage GBK1 OX=2686286 GN=GBK1_10 PE=4 SV=1
MM1 pKa = 7.43NKK3 pKa = 9.66LHH5 pKa = 6.53LHH7 pKa = 5.71VDD9 pKa = 3.41STKK12 pKa = 10.7KK13 pKa = 9.85KK14 pKa = 10.34RR15 pKa = 11.84KK16 pKa = 7.8GQLWCPYY23 pKa = 8.84CGEE26 pKa = 3.7WTYY29 pKa = 11.55FKK31 pKa = 10.25MFRR34 pKa = 11.84KK35 pKa = 9.73LRR37 pKa = 11.84FFPFQSTYY45 pKa = 10.07RR46 pKa = 11.84RR47 pKa = 11.84CVKK50 pKa = 10.47CGISVEE56 pKa = 4.26DD57 pKa = 3.88FWVKK61 pKa = 9.06TVNKK65 pKa = 9.34LWSFGVNSRR74 pKa = 11.84RR75 pKa = 11.84KK76 pKa = 9.24

Molecular weight: 9.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

13209

33

1028

235.9

26.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.7 ± 0.491

0.931 ± 0.164

5.496 ± 0.292

8.577 ± 0.589

4.467 ± 0.231

6.405 ± 0.259

1.832 ± 0.204

6.337 ± 0.228

7.987 ± 0.555

8.714 ± 0.325

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.392 ± 0.146

4.671 ± 0.23

3.407 ± 0.179

3.838 ± 0.21

5.443 ± 0.27

5.193 ± 0.533

5.368 ± 0.427

6.98 ± 0.323

1.219 ± 0.125

4.043 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski