Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) (Clostridium phytofermentans)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnoclostridium; Lachnoclostridium phytofermentans

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3891 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A9KQM4|A9KQM4_LACP7 Hydrogenase accessory protein HypB OS=Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) OX=357809 GN=Cphy_1563 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 10.22AYY4 pKa = 10.84VNEE7 pKa = 4.13DD8 pKa = 3.77CIGCGLCANSCPSVFQMTEE27 pKa = 3.28EE28 pKa = 4.34GLAKK32 pKa = 10.53AIDD35 pKa = 3.82EE36 pKa = 4.68DD37 pKa = 4.17VSKK40 pKa = 11.14DD41 pKa = 3.62LEE43 pKa = 5.59ADD45 pKa = 3.46VLDD48 pKa = 5.06AVDD51 pKa = 4.0NCPVGAIKK59 pKa = 9.99TNN61 pKa = 3.33

Molecular weight: 6.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A9KLY6|A9KLY6_LACP7 Uncharacterized protein OS=Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) OX=357809 GN=Cphy_0947 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.05VHH16 pKa = 5.81GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.74VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.21GRR39 pKa = 11.84HH40 pKa = 5.51KK41 pKa = 10.9LSAA44 pKa = 3.8

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3891

0

3891

1314630

37

2457

337.9

38.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.332 ± 0.038

1.322 ± 0.018

5.36 ± 0.027

7.317 ± 0.047

4.319 ± 0.028

6.545 ± 0.04

1.647 ± 0.017

8.445 ± 0.046

7.418 ± 0.034

9.25 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.851 ± 0.023

5.262 ± 0.031

3.107 ± 0.024

3.081 ± 0.021

3.699 ± 0.024

6.416 ± 0.033

5.742 ± 0.044

6.675 ± 0.036

0.85 ± 0.014

4.361 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski