Vibrio phage vB_VpaM_MAR

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Vhmlvirus; Vibrio virus MAR

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7RFY5|K7RFY5_9CAUD Putative exonuclease OS=Vibrio phage vB_VpaM_MAR OX=1229754 GN=MAR_34 PE=4 SV=1
MM1 pKa = 8.05EE2 pKa = 4.91YY3 pKa = 8.84RR4 pKa = 11.84TKK6 pKa = 11.08DD7 pKa = 3.1GDD9 pKa = 3.98VVDD12 pKa = 5.57DD13 pKa = 3.54ICYY16 pKa = 9.18RR17 pKa = 11.84QYY19 pKa = 11.56GRR21 pKa = 11.84SDD23 pKa = 3.41YY24 pKa = 11.3ALQVYY29 pKa = 8.06EE30 pKa = 4.95LNPGLAEE37 pKa = 3.95YY38 pKa = 10.64GPRR41 pKa = 11.84LPAGLIIQLPEE52 pKa = 3.35ISKK55 pKa = 10.61PEE57 pKa = 4.01TPEE60 pKa = 3.79LPKK63 pKa = 10.41ISLFDD68 pKa = 3.47

Molecular weight: 7.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7R9K5|K7R9K5_9CAUD Baseplate assembly protein OS=Vibrio phage vB_VpaM_MAR OX=1229754 GN=MAR_14 PE=4 SV=1
MM1 pKa = 7.32LQLNLQLDD9 pKa = 3.56QEE11 pKa = 4.44LAYY14 pKa = 11.07LEE16 pKa = 4.43AQLMASVPQIQKK28 pKa = 10.87AIDD31 pKa = 3.37RR32 pKa = 11.84ALKK35 pKa = 8.72KK36 pKa = 7.8TARR39 pKa = 11.84WLEE42 pKa = 4.06THH44 pKa = 6.48SKK46 pKa = 10.66RR47 pKa = 11.84EE48 pKa = 3.89LGRR51 pKa = 11.84EE52 pKa = 3.71LKK54 pKa = 10.96LPIKK58 pKa = 10.19VLTVRR63 pKa = 11.84FQHH66 pKa = 5.83TFYY69 pKa = 11.26LKK71 pKa = 10.7DD72 pKa = 3.24GEE74 pKa = 4.54KK75 pKa = 10.47AVSVWFGLNPVQVRR89 pKa = 11.84HH90 pKa = 6.12IGKK93 pKa = 9.46ARR95 pKa = 11.84QNRR98 pKa = 11.84RR99 pKa = 11.84GTRR102 pKa = 11.84VGKK105 pKa = 9.23HH106 pKa = 4.66QFDD109 pKa = 4.2GAFSAVMKK117 pKa = 10.62SEE119 pKa = 3.82HH120 pKa = 6.52LNVFRR125 pKa = 11.84RR126 pKa = 11.84KK127 pKa = 9.72GRR129 pKa = 11.84EE130 pKa = 3.52RR131 pKa = 11.84LPIEE135 pKa = 4.46VVRR138 pKa = 11.84LPIEE142 pKa = 4.26EE143 pKa = 3.85QSNPIFEE150 pKa = 4.96RR151 pKa = 11.84YY152 pKa = 7.15YY153 pKa = 10.72QRR155 pKa = 11.84AQGRR159 pKa = 11.84FTEE162 pKa = 4.61LLRR165 pKa = 11.84QEE167 pKa = 5.39LNFSLNHH174 pKa = 6.21EE175 pKa = 4.56GAA177 pKa = 3.78

Molecular weight: 20.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12728

41

1099

205.3

22.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.08 ± 0.646

1.234 ± 0.189

6.097 ± 0.303

7.212 ± 0.289

3.41 ± 0.192

7.33 ± 0.262

2.027 ± 0.204

4.903 ± 0.219

5.72 ± 0.323

8.894 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.381 ± 0.17

3.653 ± 0.179

3.858 ± 0.237

4.926 ± 0.343

5.523 ± 0.328

5.398 ± 0.187

5.649 ± 0.2

7.385 ± 0.261

1.485 ± 0.156

2.836 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski