Amino acid dipepetide frequency for Escherichia phage HK633

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.801AlaAla: 10.801 ± 1.564
0.736AlaCys: 0.736 ± 0.257
6.955AlaAsp: 6.955 ± 0.632
7.446AlaGlu: 7.446 ± 1.448
2.7AlaPhe: 2.7 ± 0.455
6.792AlaGly: 6.792 ± 0.854
1.637AlaHis: 1.637 ± 0.379
6.873AlaIle: 6.873 ± 0.713
5.319AlaLys: 5.319 ± 0.999
7.283AlaLeu: 7.283 ± 0.892
3.846AlaMet: 3.846 ± 0.628
5.81AlaAsn: 5.81 ± 0.597
1.555AlaPro: 1.555 ± 0.292
4.746AlaGln: 4.746 ± 0.667
5.237AlaArg: 5.237 ± 0.804
5.728AlaSer: 5.728 ± 1.178
6.137AlaThr: 6.137 ± 0.685
5.564AlaVal: 5.564 ± 0.719
2.046AlaTrp: 2.046 ± 0.381
2.537AlaTyr: 2.537 ± 0.456
0.0AlaXaa: 0.0 ± 0.0
Cys
0.982CysAla: 0.982 ± 0.353
0.164CysCys: 0.164 ± 0.103
0.9CysAsp: 0.9 ± 0.292
0.9CysGlu: 0.9 ± 0.305
0.327CysPhe: 0.327 ± 0.188
1.146CysGly: 1.146 ± 0.395
0.409CysHis: 0.409 ± 0.195
0.409CysIle: 0.409 ± 0.177
0.573CysLys: 0.573 ± 0.228
0.573CysLeu: 0.573 ± 0.211
0.082CysMet: 0.082 ± 0.082
0.573CysAsn: 0.573 ± 0.23
0.327CysPro: 0.327 ± 0.191
0.327CysGln: 0.327 ± 0.149
0.736CysArg: 0.736 ± 0.3
0.655CysSer: 0.655 ± 0.254
0.409CysThr: 0.409 ± 0.217
0.655CysVal: 0.655 ± 0.265
0.245CysTrp: 0.245 ± 0.178
0.164CysTyr: 0.164 ± 0.174
0.0CysXaa: 0.0 ± 0.0
Asp
5.973AspAla: 5.973 ± 0.706
0.573AspCys: 0.573 ± 0.27
4.173AspAsp: 4.173 ± 0.681
3.028AspGlu: 3.028 ± 0.55
1.882AspPhe: 1.882 ± 0.452
4.582AspGly: 4.582 ± 0.717
0.573AspHis: 0.573 ± 0.214
2.864AspIle: 2.864 ± 0.432
2.537AspLys: 2.537 ± 0.492
4.991AspLeu: 4.991 ± 0.564
1.391AspMet: 1.391 ± 0.292
3.028AspAsn: 3.028 ± 0.522
1.718AspPro: 1.718 ± 0.359
2.127AspGln: 2.127 ± 0.431
3.519AspArg: 3.519 ± 0.521
4.009AspSer: 4.009 ± 0.563
2.209AspThr: 2.209 ± 0.379
4.173AspVal: 4.173 ± 0.697
0.818AspTrp: 0.818 ± 0.254
2.373AspTyr: 2.373 ± 0.434
0.0AspXaa: 0.0 ± 0.0
Glu
6.137GluAla: 6.137 ± 1.215
0.655GluCys: 0.655 ± 0.267
1.964GluAsp: 1.964 ± 0.378
4.5GluGlu: 4.5 ± 1.217
2.782GluPhe: 2.782 ± 0.441
4.091GluGly: 4.091 ± 0.694
0.982GluHis: 0.982 ± 0.314
4.091GluIle: 4.091 ± 0.568
4.582GluLys: 4.582 ± 0.639
5.81GluLeu: 5.81 ± 0.595
1.637GluMet: 1.637 ± 0.387
3.191GluAsn: 3.191 ± 0.442
1.882GluPro: 1.882 ± 0.484
3.928GluGln: 3.928 ± 0.526
4.173GluArg: 4.173 ± 0.84
4.5GluSer: 4.5 ± 0.651
2.946GluThr: 2.946 ± 0.538
3.6GluVal: 3.6 ± 0.493
0.655GluTrp: 0.655 ± 0.24
1.718GluTyr: 1.718 ± 0.369
0.0GluXaa: 0.0 ± 0.0
Phe
3.028PheAla: 3.028 ± 0.631
0.409PheCys: 0.409 ± 0.179
2.373PheAsp: 2.373 ± 0.425
1.555PheGlu: 1.555 ± 0.328
0.573PhePhe: 0.573 ± 0.226
2.537PheGly: 2.537 ± 0.393
0.655PheHis: 0.655 ± 0.215
2.537PheIle: 2.537 ± 0.618
1.227PheLys: 1.227 ± 0.35
1.882PheLeu: 1.882 ± 0.426
0.9PheMet: 0.9 ± 0.243
1.718PheAsn: 1.718 ± 0.313
1.146PhePro: 1.146 ± 0.321
1.064PheGln: 1.064 ± 0.291
1.718PheArg: 1.718 ± 0.451
2.618PheSer: 2.618 ± 0.443
2.455PheThr: 2.455 ± 0.439
1.555PheVal: 1.555 ± 0.358
0.409PheTrp: 0.409 ± 0.178
1.146PheTyr: 1.146 ± 0.313
0.0PheXaa: 0.0 ± 0.0
Gly
6.546GlyAla: 6.546 ± 1.024
0.655GlyCys: 0.655 ± 0.251
3.928GlyAsp: 3.928 ± 0.581
4.419GlyGlu: 4.419 ± 0.477
2.946GlyPhe: 2.946 ± 0.482
5.237GlyGly: 5.237 ± 0.789
1.227GlyHis: 1.227 ± 0.312
3.846GlyIle: 3.846 ± 0.539
4.419GlyLys: 4.419 ± 0.548
6.055GlyLeu: 6.055 ± 0.685
2.537GlyMet: 2.537 ± 0.398
4.009GlyAsn: 4.009 ± 0.8
1.391GlyPro: 1.391 ± 0.37
3.273GlyGln: 3.273 ± 0.512
4.091GlyArg: 4.091 ± 0.653
3.437GlySer: 3.437 ± 0.568
5.155GlyThr: 5.155 ± 0.865
5.237GlyVal: 5.237 ± 0.619
1.064GlyTrp: 1.064 ± 0.35
2.455GlyTyr: 2.455 ± 0.416
0.0GlyXaa: 0.0 ± 0.0
His
1.473HisAla: 1.473 ± 0.478
0.245HisCys: 0.245 ± 0.146
0.655HisAsp: 0.655 ± 0.239
1.227HisGlu: 1.227 ± 0.397
0.245HisPhe: 0.245 ± 0.171
1.473HisGly: 1.473 ± 0.428
0.409HisHis: 0.409 ± 0.198
0.573HisIle: 0.573 ± 0.244
1.064HisLys: 1.064 ± 0.34
1.227HisLeu: 1.227 ± 0.371
0.0HisMet: 0.0 ± 0.0
0.491HisAsn: 0.491 ± 0.166
0.9HisPro: 0.9 ± 0.282
0.655HisGln: 0.655 ± 0.291
1.227HisArg: 1.227 ± 0.326
0.982HisSer: 0.982 ± 0.307
0.327HisThr: 0.327 ± 0.15
0.655HisVal: 0.655 ± 0.278
0.245HisTrp: 0.245 ± 0.147
0.409HisTyr: 0.409 ± 0.156
0.0HisXaa: 0.0 ± 0.0
Ile
5.482IleAla: 5.482 ± 0.661
0.736IleCys: 0.736 ± 0.312
4.337IleAsp: 4.337 ± 0.546
3.846IleGlu: 3.846 ± 0.624
1.964IlePhe: 1.964 ± 0.478
3.437IleGly: 3.437 ± 0.557
1.146IleHis: 1.146 ± 0.311
2.864IleIle: 2.864 ± 0.467
2.946IleLys: 2.946 ± 0.497
4.664IleLeu: 4.664 ± 0.649
1.146IleMet: 1.146 ± 0.359
3.109IleAsn: 3.109 ± 0.45
2.291IlePro: 2.291 ± 0.44
2.7IleGln: 2.7 ± 0.549
3.928IleArg: 3.928 ± 0.661
4.582IleSer: 4.582 ± 0.749
4.173IleThr: 4.173 ± 0.441
3.109IleVal: 3.109 ± 0.644
0.573IleTrp: 0.573 ± 0.195
1.718IleTyr: 1.718 ± 0.413
0.0IleXaa: 0.0 ± 0.0
Lys
6.792LysAla: 6.792 ± 1.105
0.327LysCys: 0.327 ± 0.2
2.946LysAsp: 2.946 ± 0.644
4.009LysGlu: 4.009 ± 0.754
1.227LysPhe: 1.227 ± 0.333
3.437LysGly: 3.437 ± 0.487
0.491LysHis: 0.491 ± 0.232
2.782LysIle: 2.782 ± 0.57
3.519LysLys: 3.519 ± 0.836
3.928LysLeu: 3.928 ± 0.557
2.046LysMet: 2.046 ± 0.44
2.537LysAsn: 2.537 ± 0.421
3.028LysPro: 3.028 ± 0.801
3.928LysGln: 3.928 ± 0.678
3.6LysArg: 3.6 ± 0.727
3.682LysSer: 3.682 ± 0.601
3.6LysThr: 3.6 ± 0.453
3.846LysVal: 3.846 ± 0.522
0.736LysTrp: 0.736 ± 0.258
2.291LysTyr: 2.291 ± 0.373
0.0LysXaa: 0.0 ± 0.0
Leu
8.019LeuAla: 8.019 ± 0.923
1.064LeuCys: 1.064 ± 0.343
4.5LeuAsp: 4.5 ± 0.518
5.81LeuGlu: 5.81 ± 0.767
2.127LeuPhe: 2.127 ± 0.362
3.682LeuGly: 3.682 ± 0.742
0.736LeuHis: 0.736 ± 0.268
5.319LeuIle: 5.319 ± 0.583
4.746LeuLys: 4.746 ± 0.7
6.301LeuLeu: 6.301 ± 0.819
1.718LeuMet: 1.718 ± 0.35
4.173LeuAsn: 4.173 ± 0.714
3.928LeuPro: 3.928 ± 0.533
3.273LeuGln: 3.273 ± 0.551
5.319LeuArg: 5.319 ± 0.725
6.055LeuSer: 6.055 ± 0.746
5.237LeuThr: 5.237 ± 0.686
5.401LeuVal: 5.401 ± 0.678
0.327LeuTrp: 0.327 ± 0.144
2.455LeuTyr: 2.455 ± 0.403
0.0LeuXaa: 0.0 ± 0.0
Met
3.437MetAla: 3.437 ± 0.498
0.409MetCys: 0.409 ± 0.179
1.473MetAsp: 1.473 ± 0.336
1.473MetGlu: 1.473 ± 0.38
0.573MetPhe: 0.573 ± 0.221
2.127MetGly: 2.127 ± 0.415
0.245MetHis: 0.245 ± 0.124
1.146MetIle: 1.146 ± 0.328
1.964MetLys: 1.964 ± 0.362
2.7MetLeu: 2.7 ± 0.451
0.491MetMet: 0.491 ± 0.174
0.736MetAsn: 0.736 ± 0.283
1.391MetPro: 1.391 ± 0.3
1.637MetGln: 1.637 ± 0.376
1.555MetArg: 1.555 ± 0.358
2.127MetSer: 2.127 ± 0.406
1.718MetThr: 1.718 ± 0.381
0.736MetVal: 0.736 ± 0.24
0.245MetTrp: 0.245 ± 0.149
0.736MetTyr: 0.736 ± 0.272
0.0MetXaa: 0.0 ± 0.0
Asn
5.073AsnAla: 5.073 ± 0.666
0.491AsnCys: 0.491 ± 0.227
2.537AsnAsp: 2.537 ± 0.369
2.7AsnGlu: 2.7 ± 0.429
1.391AsnPhe: 1.391 ± 0.315
5.155AsnGly: 5.155 ± 0.78
1.146AsnHis: 1.146 ± 0.338
3.355AsnIle: 3.355 ± 0.495
3.273AsnLys: 3.273 ± 0.529
3.191AsnLeu: 3.191 ± 0.489
0.573AsnMet: 0.573 ± 0.195
2.455AsnAsn: 2.455 ± 0.485
2.373AsnPro: 2.373 ± 0.491
2.291AsnGln: 2.291 ± 0.326
1.882AsnArg: 1.882 ± 0.514
2.373AsnSer: 2.373 ± 0.561
3.191AsnThr: 3.191 ± 0.569
1.718AsnVal: 1.718 ± 0.514
0.818AsnTrp: 0.818 ± 0.214
1.473AsnTyr: 1.473 ± 0.387
0.0AsnXaa: 0.0 ± 0.0
Pro
3.682ProAla: 3.682 ± 0.679
0.327ProCys: 0.327 ± 0.151
1.8ProAsp: 1.8 ± 0.459
2.618ProGlu: 2.618 ± 0.541
1.227ProPhe: 1.227 ± 0.322
3.109ProGly: 3.109 ± 0.507
0.491ProHis: 0.491 ± 0.212
1.309ProIle: 1.309 ± 0.403
1.882ProLys: 1.882 ± 0.399
2.618ProLeu: 2.618 ± 0.692
1.391ProMet: 1.391 ± 0.371
0.982ProAsn: 0.982 ± 0.318
1.8ProPro: 1.8 ± 0.404
1.882ProGln: 1.882 ± 0.444
1.964ProArg: 1.964 ± 0.386
2.946ProSer: 2.946 ± 0.633
1.637ProThr: 1.637 ± 0.448
3.273ProVal: 3.273 ± 0.485
0.409ProTrp: 0.409 ± 0.238
0.9ProTyr: 0.9 ± 0.237
0.0ProXaa: 0.0 ± 0.0
Gln
5.155GlnAla: 5.155 ± 0.858
0.327GlnCys: 0.327 ± 0.158
1.555GlnAsp: 1.555 ± 0.363
2.291GlnGlu: 2.291 ± 0.323
0.982GlnPhe: 0.982 ± 0.257
2.373GlnGly: 2.373 ± 0.52
0.491GlnHis: 0.491 ± 0.232
2.946GlnIle: 2.946 ± 0.411
3.437GlnLys: 3.437 ± 0.53
4.255GlnLeu: 4.255 ± 0.665
1.391GlnMet: 1.391 ± 0.337
2.209GlnAsn: 2.209 ± 0.562
1.473GlnPro: 1.473 ± 0.343
3.191GlnGln: 3.191 ± 0.641
3.273GlnArg: 3.273 ± 0.701
3.191GlnSer: 3.191 ± 0.713
3.519GlnThr: 3.519 ± 0.685
3.355GlnVal: 3.355 ± 0.407
0.9GlnTrp: 0.9 ± 0.338
1.555GlnTyr: 1.555 ± 0.377
0.0GlnXaa: 0.0 ± 0.0
Arg
4.828ArgAla: 4.828 ± 0.797
0.736ArgCys: 0.736 ± 0.248
3.437ArgAsp: 3.437 ± 0.549
4.746ArgGlu: 4.746 ± 0.95
2.127ArgPhe: 2.127 ± 0.344
3.109ArgGly: 3.109 ± 0.54
1.227ArgHis: 1.227 ± 0.321
3.846ArgIle: 3.846 ± 0.664
4.828ArgLys: 4.828 ± 0.696
5.646ArgLeu: 5.646 ± 0.6
2.209ArgMet: 2.209 ± 0.527
2.537ArgAsn: 2.537 ± 0.435
1.473ArgPro: 1.473 ± 0.382
2.618ArgGln: 2.618 ± 0.559
3.928ArgArg: 3.928 ± 0.89
2.782ArgSer: 2.782 ± 0.401
3.109ArgThr: 3.109 ± 0.442
3.355ArgVal: 3.355 ± 0.68
1.227ArgTrp: 1.227 ± 0.334
2.373ArgTyr: 2.373 ± 0.499
0.0ArgXaa: 0.0 ± 0.0
Ser
6.628SerAla: 6.628 ± 1.139
0.573SerCys: 0.573 ± 0.203
3.764SerAsp: 3.764 ± 0.48
3.764SerGlu: 3.764 ± 0.614
2.291SerPhe: 2.291 ± 0.374
5.973SerGly: 5.973 ± 0.865
0.573SerHis: 0.573 ± 0.248
3.437SerIle: 3.437 ± 0.456
3.028SerLys: 3.028 ± 0.789
6.382SerLeu: 6.382 ± 0.65
2.127SerMet: 2.127 ± 0.413
2.7SerAsn: 2.7 ± 0.419
2.7SerPro: 2.7 ± 0.483
3.437SerGln: 3.437 ± 0.884
4.255SerArg: 4.255 ± 0.599
5.237SerSer: 5.237 ± 0.903
3.6SerThr: 3.6 ± 0.623
5.237SerVal: 5.237 ± 0.926
1.309SerTrp: 1.309 ± 0.307
0.736SerTyr: 0.736 ± 0.217
0.0SerXaa: 0.0 ± 0.0
Thr
6.71ThrAla: 6.71 ± 0.813
0.9ThrCys: 0.9 ± 0.271
3.519ThrAsp: 3.519 ± 0.465
3.109ThrGlu: 3.109 ± 0.47
2.782ThrPhe: 2.782 ± 0.599
5.319ThrGly: 5.319 ± 0.724
0.573ThrHis: 0.573 ± 0.265
3.764ThrIle: 3.764 ± 0.584
3.273ThrLys: 3.273 ± 0.662
3.109ThrLeu: 3.109 ± 0.483
1.227ThrMet: 1.227 ± 0.255
2.209ThrAsn: 2.209 ± 0.425
2.946ThrPro: 2.946 ± 0.64
2.291ThrGln: 2.291 ± 0.482
2.782ThrArg: 2.782 ± 0.448
4.173ThrSer: 4.173 ± 0.559
3.191ThrThr: 3.191 ± 0.624
4.419ThrVal: 4.419 ± 0.48
1.146ThrTrp: 1.146 ± 0.333
1.637ThrTyr: 1.637 ± 0.378
0.0ThrXaa: 0.0 ± 0.0
Val
5.155ValAla: 5.155 ± 0.754
0.409ValCys: 0.409 ± 0.184
3.028ValAsp: 3.028 ± 0.467
4.091ValGlu: 4.091 ± 0.449
2.127ValPhe: 2.127 ± 0.325
5.073ValGly: 5.073 ± 0.784
0.736ValHis: 0.736 ± 0.243
3.928ValIle: 3.928 ± 0.61
3.928ValLys: 3.928 ± 0.503
5.973ValLeu: 5.973 ± 0.804
1.391ValMet: 1.391 ± 0.275
3.109ValAsn: 3.109 ± 0.605
2.209ValPro: 2.209 ± 0.388
2.127ValGln: 2.127 ± 0.378
3.273ValArg: 3.273 ± 0.493
5.319ValSer: 5.319 ± 0.795
3.682ValThr: 3.682 ± 0.464
5.237ValVal: 5.237 ± 0.747
1.227ValTrp: 1.227 ± 0.33
1.391ValTyr: 1.391 ± 0.34
0.0ValXaa: 0.0 ± 0.0
Trp
0.818TrpAla: 0.818 ± 0.241
0.409TrpCys: 0.409 ± 0.184
0.818TrpAsp: 0.818 ± 0.274
0.655TrpGlu: 0.655 ± 0.185
0.327TrpPhe: 0.327 ± 0.169
0.982TrpGly: 0.982 ± 0.312
0.409TrpHis: 0.409 ± 0.176
0.9TrpIle: 0.9 ± 0.263
1.064TrpLys: 1.064 ± 0.302
1.473TrpLeu: 1.473 ± 0.35
0.409TrpMet: 0.409 ± 0.164
0.655TrpAsn: 0.655 ± 0.284
0.327TrpPro: 0.327 ± 0.152
0.573TrpGln: 0.573 ± 0.227
1.227TrpArg: 1.227 ± 0.237
1.146TrpSer: 1.146 ± 0.321
0.982TrpThr: 0.982 ± 0.362
0.982TrpVal: 0.982 ± 0.237
0.491TrpTrp: 0.491 ± 0.177
0.736TrpTyr: 0.736 ± 0.213
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.028TyrAla: 3.028 ± 0.428
0.409TyrCys: 0.409 ± 0.148
1.882TyrAsp: 1.882 ± 0.312
1.637TyrGlu: 1.637 ± 0.356
0.9TyrPhe: 0.9 ± 0.328
2.291TyrGly: 2.291 ± 0.377
0.327TyrHis: 0.327 ± 0.149
1.964TyrIle: 1.964 ± 0.388
1.146TyrLys: 1.146 ± 0.324
2.127TyrLeu: 2.127 ± 0.467
0.327TyrMet: 0.327 ± 0.174
1.227TyrAsn: 1.227 ± 0.323
1.391TyrPro: 1.391 ± 0.374
1.8TyrGln: 1.8 ± 0.386
2.455TyrArg: 2.455 ± 0.383
2.209TyrSer: 2.209 ± 0.508
1.882TyrThr: 1.882 ± 0.415
1.309TyrVal: 1.309 ± 0.265
0.491TyrTrp: 0.491 ± 0.182
0.655TyrTyr: 0.655 ± 0.247
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (12222 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski