Amino acid dipepetide frequency for uncultured bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.963AlaAla: 13.963 ± 0.312
1.205AlaCys: 1.205 ± 0.062
5.878AlaAsp: 5.878 ± 0.146
6.46AlaGlu: 6.46 ± 0.143
4.091AlaPhe: 4.091 ± 0.127
8.817AlaGly: 8.817 ± 0.219
2.058AlaHis: 2.058 ± 0.076
5.859AlaIle: 5.859 ± 0.133
4.217AlaLys: 4.217 ± 0.132
11.424AlaLeu: 11.424 ± 0.189
2.592AlaMet: 2.592 ± 0.1
3.068AlaAsn: 3.068 ± 0.103
4.885AlaPro: 4.885 ± 0.143
3.677AlaGln: 3.677 ± 0.115
7.911AlaArg: 7.911 ± 0.21
5.976AlaSer: 5.976 ± 0.152
5.934AlaThr: 5.934 ± 0.144
8.141AlaVal: 8.141 ± 0.16
1.65AlaTrp: 1.65 ± 0.067
2.578AlaTyr: 2.578 ± 0.097
0.0AlaXaa: 0.0 ± 0.0
Cys
1.004CysAla: 1.004 ± 0.057
0.171CysCys: 0.171 ± 0.022
0.523CysAsp: 0.523 ± 0.043
0.503CysGlu: 0.503 ± 0.036
0.414CysPhe: 0.414 ± 0.034
1.219CysGly: 1.219 ± 0.065
0.285CysHis: 0.285 ± 0.031
0.38CysIle: 0.38 ± 0.03
0.277CysLys: 0.277 ± 0.028
1.057CysLeu: 1.057 ± 0.055
0.162CysMet: 0.162 ± 0.024
0.229CysAsn: 0.229 ± 0.026
0.512CysPro: 0.512 ± 0.047
0.302CysGln: 0.302 ± 0.027
0.831CysArg: 0.831 ± 0.054
0.607CysSer: 0.607 ± 0.038
0.439CysThr: 0.439 ± 0.037
0.761CysVal: 0.761 ± 0.043
0.204CysTrp: 0.204 ± 0.028
0.268CysTyr: 0.268 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
5.856AspAla: 5.856 ± 0.13
0.478AspCys: 0.478 ± 0.038
3.087AspAsp: 3.087 ± 0.111
3.35AspGlu: 3.35 ± 0.099
2.1AspPhe: 2.1 ± 0.082
4.729AspGly: 4.729 ± 0.15
1.211AspHis: 1.211 ± 0.068
2.374AspIle: 2.374 ± 0.087
1.829AspLys: 1.829 ± 0.074
5.657AspLeu: 5.657 ± 0.159
0.878AspMet: 0.878 ± 0.052
1.404AspAsn: 1.404 ± 0.07
3.694AspPro: 3.694 ± 0.117
1.89AspGln: 1.89 ± 0.069
4.304AspArg: 4.304 ± 0.126
2.69AspSer: 2.69 ± 0.093
2.324AspThr: 2.324 ± 0.095
3.901AspVal: 3.901 ± 0.121
0.979AspTrp: 0.979 ± 0.053
1.538AspTyr: 1.538 ± 0.067
0.0AspXaa: 0.0 ± 0.0
Glu
6.27GluAla: 6.27 ± 0.151
0.405GluCys: 0.405 ± 0.035
2.357GluAsp: 2.357 ± 0.078
3.107GluGlu: 3.107 ± 0.12
1.98GluPhe: 1.98 ± 0.076
3.63GluGly: 3.63 ± 0.122
1.345GluHis: 1.345 ± 0.064
3.473GluIle: 3.473 ± 0.108
2.864GluLys: 2.864 ± 0.131
5.959GluLeu: 5.959 ± 0.157
1.337GluMet: 1.337 ± 0.064
1.664GluAsn: 1.664 ± 0.071
2.833GluPro: 2.833 ± 0.097
2.453GluGln: 2.453 ± 0.089
4.765GluArg: 4.765 ± 0.127
2.908GluSer: 2.908 ± 0.099
2.973GluThr: 2.973 ± 0.101
3.856GluVal: 3.856 ± 0.105
0.775GluTrp: 0.775 ± 0.045
1.144GluTyr: 1.144 ± 0.062
0.0GluXaa: 0.0 ± 0.0
Phe
4.239PheAla: 4.239 ± 0.113
0.436PheCys: 0.436 ± 0.035
2.492PheAsp: 2.492 ± 0.094
2.145PheGlu: 2.145 ± 0.074
1.605PhePhe: 1.605 ± 0.088
3.445PheGly: 3.445 ± 0.107
0.903PheHis: 0.903 ± 0.05
1.683PheIle: 1.683 ± 0.078
1.135PheLys: 1.135 ± 0.066
3.747PheLeu: 3.747 ± 0.115
0.68PheMet: 0.68 ± 0.045
1.138PheAsn: 1.138 ± 0.075
1.787PhePro: 1.787 ± 0.074
1.219PheGln: 1.219 ± 0.069
2.696PheArg: 2.696 ± 0.108
2.43PheSer: 2.43 ± 0.089
2.086PheThr: 2.086 ± 0.076
2.911PheVal: 2.911 ± 0.1
0.57PheTrp: 0.57 ± 0.043
1.046PheTyr: 1.046 ± 0.062
0.0PheXaa: 0.0 ± 0.0
Gly
7.73GlyAla: 7.73 ± 0.185
0.917GlyCys: 0.917 ± 0.056
4.111GlyAsp: 4.111 ± 0.104
4.321GlyGlu: 4.321 ± 0.118
3.387GlyPhe: 3.387 ± 0.114
6.695GlyGly: 6.695 ± 0.213
1.93GlyHis: 1.93 ± 0.081
4.29GlyIle: 4.29 ± 0.137
3.708GlyLys: 3.708 ± 0.133
7.732GlyLeu: 7.732 ± 0.173
1.999GlyMet: 1.999 ± 0.085
2.539GlyAsn: 2.539 ± 0.184
3.568GlyPro: 3.568 ± 0.111
2.752GlyGln: 2.752 ± 0.083
5.82GlyArg: 5.82 ± 0.136
4.838GlySer: 4.838 ± 0.135
4.634GlyThr: 4.634 ± 0.162
5.571GlyVal: 5.571 ± 0.13
1.387GlyTrp: 1.387 ± 0.075
2.223GlyTyr: 2.223 ± 0.081
0.0GlyXaa: 0.0 ± 0.0
His
2.332HisAla: 2.332 ± 0.086
0.291HisCys: 0.291 ± 0.027
1.211HisAsp: 1.211 ± 0.063
1.298HisGlu: 1.298 ± 0.064
0.864HisPhe: 0.864 ± 0.048
1.938HisGly: 1.938 ± 0.078
0.682HisHis: 0.682 ± 0.049
0.987HisIle: 0.987 ± 0.052
0.607HisLys: 0.607 ± 0.047
2.24HisLeu: 2.24 ± 0.082
0.35HisMet: 0.35 ± 0.029
0.587HisAsn: 0.587 ± 0.032
1.505HisPro: 1.505 ± 0.072
0.685HisGln: 0.685 ± 0.047
1.865HisArg: 1.865 ± 0.079
1.172HisSer: 1.172 ± 0.066
1.024HisThr: 1.024 ± 0.05
1.577HisVal: 1.577 ± 0.07
0.439HisTrp: 0.439 ± 0.036
0.663HisTyr: 0.663 ± 0.042
0.0HisXaa: 0.0 ± 0.0
Ile
6.214IleAla: 6.214 ± 0.153
0.582IleCys: 0.582 ± 0.041
3.283IleAsp: 3.283 ± 0.093
3.317IleGlu: 3.317 ± 0.101
1.759IlePhe: 1.759 ± 0.073
4.446IleGly: 4.446 ± 0.128
1.065IleHis: 1.065 ± 0.053
2.159IleIle: 2.159 ± 0.08
1.681IleLys: 1.681 ± 0.082
4.287IleLeu: 4.287 ± 0.117
0.825IleMet: 0.825 ± 0.05
1.432IleAsn: 1.432 ± 0.074
2.62IlePro: 2.62 ± 0.079
1.46IleGln: 1.46 ± 0.067
3.557IleArg: 3.557 ± 0.099
3.126IleSer: 3.126 ± 0.101
2.682IleThr: 2.682 ± 0.1
4.041IleVal: 4.041 ± 0.126
0.688IleTrp: 0.688 ± 0.048
1.289IleTyr: 1.289 ± 0.061
0.0IleXaa: 0.0 ± 0.0
Lys
4.142LysAla: 4.142 ± 0.123
0.263LysCys: 0.263 ± 0.03
1.837LysAsp: 1.837 ± 0.092
2.276LysGlu: 2.276 ± 0.115
1.107LysPhe: 1.107 ± 0.063
2.584LysGly: 2.584 ± 0.098
0.777LysHis: 0.777 ± 0.05
2.262LysIle: 2.262 ± 0.086
1.972LysLys: 1.972 ± 0.106
3.753LysLeu: 3.753 ± 0.119
0.85LysMet: 0.85 ± 0.053
1.275LysAsn: 1.275 ± 0.063
2.369LysPro: 2.369 ± 0.098
1.432LysGln: 1.432 ± 0.065
2.729LysArg: 2.729 ± 0.099
2.139LysSer: 2.139 ± 0.077
2.366LysThr: 2.366 ± 0.084
2.699LysVal: 2.699 ± 0.103
0.584LysTrp: 0.584 ± 0.036
0.817LysTyr: 0.817 ± 0.055
0.0LysXaa: 0.0 ± 0.0
Leu
12.405LeuAla: 12.405 ± 0.216
1.127LeuCys: 1.127 ± 0.061
5.635LeuAsp: 5.635 ± 0.132
5.333LeuGlu: 5.333 ± 0.147
3.529LeuPhe: 3.529 ± 0.116
7.953LeuGly: 7.953 ± 0.164
2.083LeuHis: 2.083 ± 0.076
4.919LeuIle: 4.919 ± 0.143
3.89LeuLys: 3.89 ± 0.151
9.609LeuLeu: 9.609 ± 0.234
1.921LeuMet: 1.921 ± 0.065
2.892LeuAsn: 2.892 ± 0.101
5.61LeuPro: 5.61 ± 0.136
3.182LeuGln: 3.182 ± 0.111
7.858LeuArg: 7.858 ± 0.167
5.758LeuSer: 5.758 ± 0.125
5.504LeuThr: 5.504 ± 0.137
7.098LeuVal: 7.098 ± 0.163
1.337LeuTrp: 1.337 ± 0.067
2.008LeuTyr: 2.008 ± 0.086
0.0LeuXaa: 0.0 ± 0.0
Met
2.137MetAla: 2.137 ± 0.087
0.131MetCys: 0.131 ± 0.02
0.825MetAsp: 0.825 ± 0.052
1.035MetGlu: 1.035 ± 0.058
0.758MetPhe: 0.758 ± 0.056
1.376MetGly: 1.376 ± 0.075
0.481MetHis: 0.481 ± 0.039
1.096MetIle: 1.096 ± 0.055
1.169MetLys: 1.169 ± 0.061
2.033MetLeu: 2.033 ± 0.091
0.492MetMet: 0.492 ± 0.041
0.8MetAsn: 0.8 ± 0.054
1.331MetPro: 1.331 ± 0.066
0.71MetGln: 0.71 ± 0.042
1.661MetArg: 1.661 ± 0.073
1.387MetSer: 1.387 ± 0.065
1.485MetThr: 1.485 ± 0.067
1.387MetVal: 1.387 ± 0.067
0.162MetTrp: 0.162 ± 0.018
0.358MetTyr: 0.358 ± 0.033
0.0MetXaa: 0.0 ± 0.0
Asn
3.048AsnAla: 3.048 ± 0.107
0.235AsnCys: 0.235 ± 0.026
1.505AsnAsp: 1.505 ± 0.069
1.404AsnGlu: 1.404 ± 0.061
1.272AsnPhe: 1.272 ± 0.064
2.707AsnGly: 2.707 ± 0.151
0.649AsnHis: 0.649 ± 0.044
1.499AsnIle: 1.499 ± 0.085
0.85AsnLys: 0.85 ± 0.056
2.869AsnLeu: 2.869 ± 0.088
0.543AsnMet: 0.543 ± 0.04
0.996AsnAsn: 0.996 ± 0.086
2.12AsnPro: 2.12 ± 0.084
1.205AsnGln: 1.205 ± 0.07
2.097AsnArg: 2.097 ± 0.081
1.776AsnSer: 1.776 ± 0.086
1.524AsnThr: 1.524 ± 0.083
2.164AsnVal: 2.164 ± 0.095
0.529AsnTrp: 0.529 ± 0.031
0.873AsnTyr: 0.873 ± 0.053
0.0AsnXaa: 0.0 ± 0.0
Pro
5.909ProAla: 5.909 ± 0.158
0.431ProCys: 0.431 ± 0.032
3.661ProAsp: 3.661 ± 0.12
3.582ProGlu: 3.582 ± 0.099
2.083ProPhe: 2.083 ± 0.083
4.832ProGly: 4.832 ± 0.136
1.172ProHis: 1.172 ± 0.06
2.453ProIle: 2.453 ± 0.096
2.164ProLys: 2.164 ± 0.093
4.642ProLeu: 4.642 ± 0.122
1.088ProMet: 1.088 ± 0.052
1.784ProAsn: 1.784 ± 0.072
3.328ProPro: 3.328 ± 0.146
1.739ProGln: 1.739 ± 0.073
3.512ProArg: 3.512 ± 0.117
3.314ProSer: 3.314 ± 0.124
3.199ProThr: 3.199 ± 0.123
3.915ProVal: 3.915 ± 0.108
0.808ProTrp: 0.808 ± 0.05
1.191ProTyr: 1.191 ± 0.06
0.0ProXaa: 0.0 ± 0.0
Gln
3.781GlnAla: 3.781 ± 0.119
0.282GlnCys: 0.282 ± 0.028
1.404GlnAsp: 1.404 ± 0.063
1.661GlnGlu: 1.661 ± 0.068
1.323GlnPhe: 1.323 ± 0.061
2.265GlnGly: 2.265 ± 0.093
0.786GlnHis: 0.786 ± 0.043
1.983GlnIle: 1.983 ± 0.084
1.412GlnLys: 1.412 ± 0.07
3.619GlnLeu: 3.619 ± 0.108
0.817GlnMet: 0.817 ± 0.051
0.984GlnAsn: 0.984 ± 0.059
2.039GlnPro: 2.039 ± 0.083
1.563GlnGln: 1.563 ± 0.08
2.805GlnArg: 2.805 ± 0.108
2.081GlnSer: 2.081 ± 0.081
2.027GlnThr: 2.027 ± 0.079
2.486GlnVal: 2.486 ± 0.091
0.537GlnTrp: 0.537 ± 0.043
0.761GlnTyr: 0.761 ± 0.048
0.0GlnXaa: 0.0 ± 0.0
Arg
7.615ArgAla: 7.615 ± 0.186
0.836ArgCys: 0.836 ± 0.052
4.323ArgAsp: 4.323 ± 0.14
4.505ArgGlu: 4.505 ± 0.116
3.113ArgPhe: 3.113 ± 0.104
5.182ArgGly: 5.182 ± 0.124
1.972ArgHis: 1.972 ± 0.09
3.753ArgIle: 3.753 ± 0.109
2.771ArgLys: 2.771 ± 0.098
8.073ArgLeu: 8.073 ± 0.183
1.695ArgMet: 1.695 ± 0.071
2.106ArgAsn: 2.106 ± 0.08
3.848ArgPro: 3.848 ± 0.106
2.836ArgGln: 2.836 ± 0.098
6.396ArgArg: 6.396 ± 0.182
4.2ArgSer: 4.2 ± 0.117
3.814ArgThr: 3.814 ± 0.104
4.888ArgVal: 4.888 ± 0.117
1.314ArgTrp: 1.314 ± 0.063
1.969ArgTyr: 1.969 ± 0.076
0.0ArgXaa: 0.0 ± 0.0
Ser
5.943SerAla: 5.943 ± 0.153
0.557SerCys: 0.557 ± 0.04
3.107SerAsp: 3.107 ± 0.085
2.959SerGlu: 2.959 ± 0.1
2.408SerPhe: 2.408 ± 0.088
5.428SerGly: 5.428 ± 0.148
1.264SerHis: 1.264 ± 0.063
2.766SerIle: 2.766 ± 0.1
2.019SerLys: 2.019 ± 0.085
5.568SerLeu: 5.568 ± 0.14
1.258SerMet: 1.258 ± 0.055
1.77SerAsn: 1.77 ± 0.079
3.627SerPro: 3.627 ± 0.113
1.977SerGln: 1.977 ± 0.078
4.066SerArg: 4.066 ± 0.109
3.515SerSer: 3.515 ± 0.132
2.948SerThr: 2.948 ± 0.101
4.259SerVal: 4.259 ± 0.109
0.973SerTrp: 0.973 ± 0.056
1.496SerTyr: 1.496 ± 0.07
0.0SerXaa: 0.0 ± 0.0
Thr
5.973ThrAla: 5.973 ± 0.142
0.515ThrCys: 0.515 ± 0.039
2.693ThrAsp: 2.693 ± 0.101
2.542ThrGlu: 2.542 ± 0.075
2.125ThrPhe: 2.125 ± 0.073
4.846ThrGly: 4.846 ± 0.146
1.121ThrHis: 1.121 ± 0.06
3.118ThrIle: 3.118 ± 0.121
1.795ThrLys: 1.795 ± 0.078
5.487ThrLeu: 5.487 ± 0.151
1.088ThrMet: 1.088 ± 0.067
1.642ThrAsn: 1.642 ± 0.075
3.554ThrPro: 3.554 ± 0.123
1.633ThrGln: 1.633 ± 0.076
3.49ThrArg: 3.49 ± 0.102
3.202ThrSer: 3.202 ± 0.113
3.113ThrThr: 3.113 ± 0.103
4.248ThrVal: 4.248 ± 0.106
0.873ThrTrp: 0.873 ± 0.048
1.214ThrTyr: 1.214 ± 0.063
0.0ThrXaa: 0.0 ± 0.0
Val
8.152ValAla: 8.152 ± 0.162
0.808ValCys: 0.808 ± 0.053
3.882ValAsp: 3.882 ± 0.114
4.259ValGlu: 4.259 ± 0.124
2.71ValPhe: 2.71 ± 0.092
5.218ValGly: 5.218 ± 0.129
1.485ValHis: 1.485 ± 0.075
3.859ValIle: 3.859 ± 0.126
2.494ValLys: 2.494 ± 0.095
7.372ValLeu: 7.372 ± 0.162
1.449ValMet: 1.449 ± 0.063
2.201ValAsn: 2.201 ± 0.081
3.789ValPro: 3.789 ± 0.119
2.251ValGln: 2.251 ± 0.089
5.308ValArg: 5.308 ± 0.139
4.349ValSer: 4.349 ± 0.116
4.184ValThr: 4.184 ± 0.132
5.722ValVal: 5.722 ± 0.158
1.093ValTrp: 1.093 ± 0.062
1.762ValTyr: 1.762 ± 0.077
0.0ValXaa: 0.0 ± 0.0
Trp
1.292TrpAla: 1.292 ± 0.07
0.218TrpCys: 0.218 ± 0.024
0.914TrpAsp: 0.914 ± 0.05
0.721TrpGlu: 0.721 ± 0.047
0.64TrpPhe: 0.64 ± 0.049
0.878TrpGly: 0.878 ± 0.052
0.389TrpHis: 0.389 ± 0.038
0.777TrpIle: 0.777 ± 0.051
0.638TrpLys: 0.638 ± 0.041
1.812TrpLeu: 1.812 ± 0.071
0.392TrpMet: 0.392 ± 0.033
0.618TrpAsn: 0.618 ± 0.041
0.666TrpPro: 0.666 ± 0.048
0.696TrpGln: 0.696 ± 0.046
1.437TrpArg: 1.437 ± 0.068
1.024TrpSer: 1.024 ± 0.056
0.875TrpThr: 0.875 ± 0.05
0.99TrpVal: 0.99 ± 0.059
0.294TrpTrp: 0.294 ± 0.031
0.375TrpTyr: 0.375 ± 0.036
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.383TyrAla: 2.383 ± 0.082
0.26TyrCys: 0.26 ± 0.028
1.546TyrAsp: 1.546 ± 0.07
1.32TyrGlu: 1.32 ± 0.069
1.127TyrPhe: 1.127 ± 0.055
1.963TyrGly: 1.963 ± 0.084
0.657TyrHis: 0.657 ± 0.042
0.822TyrIle: 0.822 ± 0.048
0.741TyrLys: 0.741 ± 0.049
2.71TyrLeu: 2.71 ± 0.096
0.397TyrMet: 0.397 ± 0.035
0.735TyrAsn: 0.735 ± 0.045
1.124TyrPro: 1.124 ± 0.053
0.951TyrGln: 0.951 ± 0.054
2.1TyrArg: 2.1 ± 0.091
1.415TyrSer: 1.415 ± 0.056
1.138TyrThr: 1.138 ± 0.069
1.731TyrVal: 1.731 ± 0.067
0.45TyrTrp: 0.45 ± 0.035
0.78TyrTyr: 0.78 ± 0.052
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1290 proteins (357591 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski