Puerto Almendras virus
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|X4R562|X4R562_9RHAB P protein OS=Puerto Almendras virus OX=1479613 GN=P PE=4 SV=1
MM1 pKa = 7.43 SRR3 pKa = 11.84 NHH5 pKa = 5.87 FQKK8 pKa = 10.77 LGNKK12 pKa = 9.25 PDD14 pKa = 3.59 IQADD18 pKa = 3.54 IYY20 pKa = 11.62 DD21 pKa = 4.12 SANLMSQINLNAYY34 pKa = 7.05 TISKK38 pKa = 9.95 RR39 pKa = 11.84 ISEE42 pKa = 4.88 DD43 pKa = 3.4 YY44 pKa = 10.32 QATRR48 pKa = 11.84 EE49 pKa = 4.04 NEE51 pKa = 4.08 EE52 pKa = 3.86 IDD54 pKa = 4.22 DD55 pKa = 3.74 INYY58 pKa = 10.14 EE59 pKa = 3.99 KK60 pKa = 10.82 EE61 pKa = 4.17 KK62 pKa = 10.63 EE63 pKa = 4.35 SKK65 pKa = 10.26 EE66 pKa = 3.95 LEE68 pKa = 4.23 KK69 pKa = 10.68 MFTEE73 pKa = 4.59 NDD75 pKa = 3.1 GDD77 pKa = 4.18 TNITPEE83 pKa = 3.7 ILQEE87 pKa = 3.95 IKK89 pKa = 10.43 RR90 pKa = 11.84 NHH92 pKa = 6.47 HH93 pKa = 6.23 LRR95 pKa = 11.84 SGTIDD100 pKa = 3.5 IIGQSTTSSNRR111 pKa = 11.84 PYY113 pKa = 11.4 LDD115 pKa = 2.98 MGKK118 pKa = 10.07 KK119 pKa = 9.54 KK120 pKa = 10.78 DD121 pKa = 4.01 EE122 pKa = 4.53 INLTSNDD129 pKa = 4.37 CIDD132 pKa = 4.05 NLNQKK137 pKa = 10.29 LEE139 pKa = 4.14 EE140 pKa = 4.92 PNTSSSRR147 pKa = 11.84 TTKK150 pKa = 10.44 FDD152 pKa = 3.05 VDD154 pKa = 3.49 YY155 pKa = 9.58 TAVNRR160 pKa = 11.84 NDD162 pKa = 3.36 YY163 pKa = 10.16 YY164 pKa = 10.79 RR165 pKa = 11.84 GYY167 pKa = 10.91 LKK169 pKa = 10.77 ALSDD173 pKa = 3.98 VNKK176 pKa = 10.5 LLIDD180 pKa = 3.49 NSINCKK186 pKa = 9.98 FDD188 pKa = 3.48 GSHH191 pKa = 6.65 SDD193 pKa = 3.17 NLKK196 pKa = 10.35 IILFAKK202 pKa = 9.12 TDD204 pKa = 3.84 PEE206 pKa = 3.97 ITSPISIEE214 pKa = 4.02 RR215 pKa = 11.84 QSQSSNKK222 pKa = 8.93 TKK224 pKa = 10.37 ILQQSTKK231 pKa = 10.4 DD232 pKa = 3.27 SEE234 pKa = 4.27 YY235 pKa = 10.63 TIRR238 pKa = 11.84 KK239 pKa = 9.41 LSDD242 pKa = 3.18 QIKK245 pKa = 10.35 VITFDD250 pKa = 3.61 TPFGEE255 pKa = 4.19 PVVIDD260 pKa = 3.44 TAKK263 pKa = 10.66 LIEE266 pKa = 4.46 SASTYY271 pKa = 10.5 LDD273 pKa = 3.22 SQAAVCQLIEE283 pKa = 4.8 LIEE286 pKa = 4.59 SNSNEE291 pKa = 4.32 SNHH294 pKa = 5.34 PTILKK299 pKa = 9.79 IISGIPKK306 pKa = 10.12 YY307 pKa = 10.67 SGLKK311 pKa = 8.22 YY312 pKa = 10.33 QVDD315 pKa = 3.25 RR316 pKa = 11.84 KK317 pKa = 10.7 YY318 pKa = 10.81 RR319 pKa = 11.84 VV320 pKa = 2.89
Molecular weight: 36.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.122
IPC2_protein 5.181
IPC_protein 5.118
Toseland 5.054
ProMoST 5.27
Dawson 5.118
Bjellqvist 5.245
Wikipedia 5.003
Rodwell 5.029
Grimsley 4.978
Solomon 5.118
Lehninger 5.08
Nozaki 5.245
DTASelect 5.423
Thurlkill 5.067
EMBOSS 5.041
Sillero 5.308
Patrickios 4.177
IPC_peptide 5.13
IPC2_peptide 5.296
IPC2.peptide.svr19 5.364
Protein with the highest isoelectric point:
>tr|X4R286|X4R286_9RHAB Nucleocapsid protein OS=Puerto Almendras virus OX=1479613 GN=N PE=4 SV=1
MM1 pKa = 7.45 FKK3 pKa = 10.64 NSYY6 pKa = 10.49 IMTGDD11 pKa = 3.19 YY12 pKa = 10.24 SVRR15 pKa = 11.84 VFKK18 pKa = 10.49 IRR20 pKa = 11.84 YY21 pKa = 6.36 EE22 pKa = 4.25 SSFEE26 pKa = 4.04 TLPSDD31 pKa = 4.57 SDD33 pKa = 3.42 IKK35 pKa = 11.19 PMIEE39 pKa = 4.16 HH40 pKa = 7.24 FDD42 pKa = 3.6 GKK44 pKa = 11.0 LKK46 pKa = 10.44 FLEE49 pKa = 4.3 VFTFILNYY57 pKa = 9.63 MVTLQIRR64 pKa = 11.84 NKK66 pKa = 10.01 VKK68 pKa = 9.48 TAPTSIFVIKK78 pKa = 10.31 FPVDD82 pKa = 3.6 SRR84 pKa = 11.84 WMFKK88 pKa = 10.49 KK89 pKa = 10.22 EE90 pKa = 3.81 EE91 pKa = 4.28 MYY93 pKa = 11.42 SMHH96 pKa = 7.06 KK97 pKa = 10.39 DD98 pKa = 2.68 IYY100 pKa = 10.53 VEE102 pKa = 4.0 NKK104 pKa = 9.62 EE105 pKa = 4.0 GSHH108 pKa = 6.23 RR109 pKa = 11.84 LRR111 pKa = 11.84 VKK113 pKa = 10.61 LVVDD117 pKa = 3.36 LHH119 pKa = 7.01 VDD121 pKa = 3.04 NRR123 pKa = 11.84 PRR125 pKa = 11.84 DD126 pKa = 3.64 RR127 pKa = 11.84 LVRR130 pKa = 11.84 PVMKK134 pKa = 10.48 KK135 pKa = 9.74 ILSKK139 pKa = 10.99 LYY141 pKa = 8.93 DD142 pKa = 3.44 TSAEE146 pKa = 3.7 RR147 pKa = 11.84 KK148 pKa = 8.46 MYY150 pKa = 10.57 EE151 pKa = 3.53 IMKK154 pKa = 10.09 EE155 pKa = 4.0 NNEE158 pKa = 4.28 CC159 pKa = 4.12
Molecular weight: 19.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.822
IPC2_protein 8.799
IPC_protein 8.712
Toseland 9.472
ProMoST 9.209
Dawson 9.736
Bjellqvist 9.399
Wikipedia 9.897
Rodwell 10.072
Grimsley 9.809
Solomon 9.78
Lehninger 9.736
Nozaki 9.487
DTASelect 9.385
Thurlkill 9.56
EMBOSS 9.897
Sillero 9.648
Patrickios 5.219
IPC_peptide 9.765
IPC2_peptide 7.892
IPC2.peptide.svr19 7.838
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3522
80
2059
587.0
68.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.612 ± 0.444
1.562 ± 0.407
6.019 ± 0.4
6.019 ± 0.449
4.287 ± 0.445
4.373 ± 0.607
2.129 ± 0.382
9.881 ± 0.514
7.95 ± 0.422
9.256 ± 0.665
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.555 ± 0.425
8.092 ± 0.5
3.18 ± 0.332
2.612 ± 0.359
4.798 ± 0.373
7.411 ± 0.828
5.679 ± 0.513
4.969 ± 0.45
1.107 ± 0.205
5.508 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here