Sewage-associated circular DNA virus-16

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UI40|A0A0B4UI40_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-16 OX=1592083 PE=4 SV=1
MM1 pKa = 7.88KK2 pKa = 10.45ARR4 pKa = 11.84FWILTIPHH12 pKa = 7.08ADD14 pKa = 3.53YY15 pKa = 11.44LPFLPNCCVWIRR27 pKa = 11.84GQLEE31 pKa = 3.9RR32 pKa = 11.84GSATGYY38 pKa = 9.14LHH40 pKa = 6.05WQLVVGFKK48 pKa = 8.46QQCRR52 pKa = 11.84LATLKK57 pKa = 9.97RR58 pKa = 11.84TFGDD62 pKa = 3.41SCHH65 pKa = 6.99AEE67 pKa = 4.05PTRR70 pKa = 11.84SNAADD75 pKa = 3.83DD76 pKa = 4.54YY77 pKa = 9.8VWKK80 pKa = 9.98PEE82 pKa = 3.73SRR84 pKa = 11.84IDD86 pKa = 3.42GTQFEE91 pKa = 5.15LGAKK95 pKa = 9.52PMRR98 pKa = 11.84RR99 pKa = 11.84NDD101 pKa = 3.22SRR103 pKa = 11.84DD104 pKa = 2.5WDD106 pKa = 4.59AIRR109 pKa = 11.84DD110 pKa = 3.62AAKK113 pKa = 10.49RR114 pKa = 11.84GDD116 pKa = 4.16LEE118 pKa = 4.52TVPSDD123 pKa = 3.75VFVQHH128 pKa = 5.61YY129 pKa = 9.77RR130 pKa = 11.84SLRR133 pKa = 11.84TISADD138 pKa = 3.22YY139 pKa = 10.53AEE141 pKa = 4.73PVAIEE146 pKa = 4.26RR147 pKa = 11.84TVHH150 pKa = 6.01VYY152 pKa = 9.12WGPTGVGKK160 pKa = 10.18SRR162 pKa = 11.84KK163 pKa = 7.33VWEE166 pKa = 4.28LAGEE170 pKa = 4.1DD171 pKa = 4.81AYY173 pKa = 10.19PKK175 pKa = 10.52CPNSKK180 pKa = 9.67FWDD183 pKa = 4.67GYY185 pKa = 9.57RR186 pKa = 11.84GHH188 pKa = 6.67TNVVIDD194 pKa = 4.01EE195 pKa = 4.09FRR197 pKa = 11.84GRR199 pKa = 11.84IDD201 pKa = 2.92ISHH204 pKa = 7.23ILRR207 pKa = 11.84WFDD210 pKa = 3.54RR211 pKa = 11.84YY212 pKa = 9.7PVLVEE217 pKa = 3.9IKK219 pKa = 10.53GSSTVLRR226 pKa = 11.84AQNIWITSNLDD237 pKa = 3.02PRR239 pKa = 11.84EE240 pKa = 4.15WYY242 pKa = 9.2TDD244 pKa = 3.45LDD246 pKa = 4.21EE247 pKa = 4.44LTKK250 pKa = 10.81LALLRR255 pKa = 11.84RR256 pKa = 11.84LNVIHH261 pKa = 7.14CPLALYY267 pKa = 10.08

Molecular weight: 30.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UI40|A0A0B4UI40_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-16 OX=1592083 PE=4 SV=1
MM1 pKa = 8.08PKK3 pKa = 9.97RR4 pKa = 11.84KK5 pKa = 9.65SSSKK9 pKa = 9.97RR10 pKa = 11.84SSSSKK15 pKa = 9.16RR16 pKa = 11.84RR17 pKa = 11.84SRR19 pKa = 11.84KK20 pKa = 9.58SSMSKK25 pKa = 10.18KK26 pKa = 9.74AIKK29 pKa = 10.15RR30 pKa = 11.84SVLQAVLGMSEE41 pKa = 3.71TKK43 pKa = 10.21YY44 pKa = 10.7RR45 pKa = 11.84QFADD49 pKa = 3.31EE50 pKa = 4.3NVNIFHH56 pKa = 6.65NTTTITGNAINMLQTSQGASDD77 pKa = 3.62NQRR80 pKa = 11.84IGDD83 pKa = 4.36TIRR86 pKa = 11.84AKK88 pKa = 10.57SLNIKK93 pKa = 8.38MQLFNKK99 pKa = 9.29QDD101 pKa = 3.73RR102 pKa = 11.84PNVTYY107 pKa = 10.24RR108 pKa = 11.84VLILAPLTTGVTPAPTIWKK127 pKa = 9.74GEE129 pKa = 4.28SGNKK133 pKa = 8.95MLDD136 pKa = 3.75NIDD139 pKa = 3.44TGSYY143 pKa = 9.85KK144 pKa = 10.04IIYY147 pKa = 8.92QKK149 pKa = 10.04FVKK152 pKa = 9.57WSSQTTTFSGPTTLPNYY169 pKa = 10.22SRR171 pKa = 11.84EE172 pKa = 3.88TSKK175 pKa = 10.65IISINIPLKK184 pKa = 10.53NKK186 pKa = 9.6KK187 pKa = 9.19LQYY190 pKa = 8.2STNSAVVPKK199 pKa = 10.34SSKK202 pKa = 10.92LNWTLYY208 pKa = 6.86VTPYY212 pKa = 10.89DD213 pKa = 3.71AFGTLITDD221 pKa = 4.6NIASYY226 pKa = 10.69AYY228 pKa = 10.12NARR231 pKa = 11.84LYY233 pKa = 10.94FKK235 pKa = 10.78DD236 pKa = 3.82FF237 pKa = 3.62

Molecular weight: 26.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

504

237

267

252.0

28.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.349 ± 0.509

1.19 ± 0.702

5.754 ± 1.153

3.373 ± 0.993

3.373 ± 0.001

5.159 ± 0.554

1.786 ± 0.804

6.548 ± 0.617

7.143 ± 1.759

7.937 ± 0.699

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.587 ± 0.557

5.159 ± 1.436

4.762 ± 0.32

3.373 ± 0.499

7.54 ± 1.211

8.532 ± 2.184

7.738 ± 1.408

5.556 ± 0.788

2.778 ± 0.891

4.365 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski