Wenzhou tombus-like virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGS9|A0A1L3KGS9_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 3 OX=1923673 PE=4 SV=1
MM1 pKa = 7.96CILILLCVAFLALVFKK17 pKa = 10.6LVYY20 pKa = 9.87RR21 pKa = 11.84SRR23 pKa = 11.84EE24 pKa = 3.73SVYY27 pKa = 10.87RR28 pKa = 11.84EE29 pKa = 3.63DD30 pKa = 3.16VDD32 pKa = 4.55RR33 pKa = 11.84FDD35 pKa = 4.08RR36 pKa = 11.84ACEE39 pKa = 3.94GGEE42 pKa = 3.96IPDD45 pKa = 3.25GRR47 pKa = 11.84VFTRR51 pKa = 11.84LAFEE55 pKa = 4.29AACACKK61 pKa = 10.07EE62 pKa = 4.01KK63 pKa = 10.67YY64 pKa = 10.83GEE66 pKa = 3.95MRR68 pKa = 11.84YY69 pKa = 10.53NNANRR74 pKa = 11.84IVVGEE79 pKa = 4.05FVRR82 pKa = 11.84EE83 pKa = 4.09YY84 pKa = 11.0LRR86 pKa = 11.84KK87 pKa = 8.02TYY89 pKa = 10.44PDD91 pKa = 3.43LRR93 pKa = 11.84VVDD96 pKa = 5.36RR97 pKa = 11.84IKK99 pKa = 10.38HH100 pKa = 4.12ATYY103 pKa = 10.42AVEE106 pKa = 4.81LALLPMKK113 pKa = 10.42SAVVAQEE120 pKa = 3.72FAADD124 pKa = 3.67VEE126 pKa = 4.99VVGRR130 pKa = 11.84RR131 pKa = 11.84TAGYY135 pKa = 7.6ATRR138 pKa = 4.62

Molecular weight: 15.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGS3|A0A1L3KGS3_9VIRU Uncharacterized protein OS=Wenzhou tombus-like virus 3 OX=1923673 PE=4 SV=1
MM1 pKa = 7.23TRR3 pKa = 11.84GTPVPGGVRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84VRR17 pKa = 11.84MPMLSGSGTNAIIKK31 pKa = 9.91YY32 pKa = 8.95SALGGSLTTGSGTNQGTNARR52 pKa = 11.84YY53 pKa = 9.2YY54 pKa = 9.32IPGYY58 pKa = 10.96AGDD61 pKa = 3.99LTNAAGAAVVSYY73 pKa = 10.98YY74 pKa = 10.08STAKK78 pKa = 10.07FLPGTRR84 pKa = 11.84VKK86 pKa = 10.12WEE88 pKa = 3.91PSVSFTTAGRR98 pKa = 11.84VIVGFTDD105 pKa = 3.15NPEE108 pKa = 4.07VVTTLNTLATNAPTNYY124 pKa = 9.49SAYY127 pKa = 9.04INAVRR132 pKa = 11.84SLGNVVSFPVWQEE145 pKa = 3.66TEE147 pKa = 3.87IPFPTRR153 pKa = 11.84LRR155 pKa = 11.84RR156 pKa = 11.84KK157 pKa = 9.82RR158 pKa = 11.84FDD160 pKa = 3.4INASPAGSNADD171 pKa = 3.29QMDD174 pKa = 4.0RR175 pKa = 11.84SAQTVMFVAVEE186 pKa = 4.19GAPATTSLGSFWYY199 pKa = 10.05HH200 pKa = 6.57DD201 pKa = 3.85VVDD204 pKa = 4.13VEE206 pKa = 4.7GVTGITTT213 pKa = 3.68

Molecular weight: 22.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

815

138

464

271.7

30.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.712 ± 1.389

2.086 ± 0.743

4.663 ± 0.499

5.031 ± 1.08

4.294 ± 0.24

8.098 ± 0.956

0.859 ± 0.177

3.313 ± 0.235

2.699 ± 0.467

7.73 ± 1.097

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.086 ± 0.118

3.926 ± 0.648

5.031 ± 0.794

2.331 ± 0.603

9.693 ± 0.942

6.258 ± 1.14

6.38 ± 2.062

11.043 ± 0.369

1.718 ± 0.601

4.049 ± 0.58
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski