Faecalibacterium phage FP_Mushu

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Mushuvirus; Faecalibacterium virus Mushu

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9VH12|A0A2K9VH12_9CAUD Uncharacterized protein OS=Faecalibacterium phage FP_Mushu OX=2070185 PE=4 SV=1
MM1 pKa = 7.54TGLNDD6 pKa = 3.29TDD8 pKa = 3.12IRR10 pKa = 11.84LNSEE14 pKa = 3.76WQLTQATDD22 pKa = 3.46GDD24 pKa = 4.3APLCSGLEE32 pKa = 3.8CLYY35 pKa = 11.06QNIVLEE41 pKa = 4.62ALTQPGDD48 pKa = 3.51VFYY51 pKa = 10.83DD52 pKa = 3.71AEE54 pKa = 4.65FGWGLYY60 pKa = 10.66DD61 pKa = 5.32FIQSEE66 pKa = 4.49DD67 pKa = 3.77TEE69 pKa = 4.4LTRR72 pKa = 11.84LEE74 pKa = 3.52ITQRR78 pKa = 11.84VRR80 pKa = 11.84LKK82 pKa = 9.3LQKK85 pKa = 10.28RR86 pKa = 11.84EE87 pKa = 4.16VILPEE92 pKa = 4.36SIEE95 pKa = 3.75ISIAFEE101 pKa = 4.58DD102 pKa = 4.24DD103 pKa = 3.96AVVLHH108 pKa = 6.37CSFRR112 pKa = 11.84FAEE115 pKa = 4.02EE116 pKa = 3.93DD117 pKa = 3.18EE118 pKa = 4.25RR119 pKa = 11.84RR120 pKa = 11.84EE121 pKa = 3.74LDD123 pKa = 3.74VIIGAVSVEE132 pKa = 4.46VVSEE136 pKa = 4.0

Molecular weight: 15.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9VGY9|A0A2K9VGY9_9CAUD Inorganic polyphosphate kinase OS=Faecalibacterium phage FP_Mushu OX=2070185 PE=4 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84GEE4 pKa = 4.12TAGKK8 pKa = 7.61PLKK11 pKa = 10.45YY12 pKa = 10.21AGNGDD17 pKa = 3.65FRR19 pKa = 11.84RR20 pKa = 11.84GTEE23 pKa = 3.53ARR25 pKa = 11.84IINNLEE31 pKa = 3.18MRR33 pKa = 11.84MRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84SALFSRR44 pKa = 11.84PRR46 pKa = 11.84GRR48 pKa = 11.84TTAAARR54 pKa = 11.84RR55 pKa = 11.84GAHH58 pKa = 5.63IRR60 pKa = 11.84RR61 pKa = 11.84IQKK64 pKa = 9.84SPGNARR70 pKa = 11.84ITAFLRR76 pKa = 11.84LLDD79 pKa = 3.95GMSRR83 pKa = 11.84SAPSRR88 pKa = 11.84KK89 pKa = 9.19SQQQTKK95 pKa = 7.63RR96 pKa = 11.84TRR98 pKa = 11.84ASRR101 pKa = 11.84LMLLLL106 pKa = 4.29

Molecular weight: 12.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

11561

46

795

214.1

23.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.809 ± 0.385

0.813 ± 0.103

6.038 ± 0.305

8.036 ± 0.332

3.529 ± 0.206

7.24 ± 0.342

1.272 ± 0.131

5.631 ± 0.232

7.032 ± 0.333

8.459 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.396 ± 0.148

3.59 ± 0.236

3.572 ± 0.217

3.477 ± 0.177

5.89 ± 0.468

5.605 ± 0.358

6.288 ± 0.363

6.842 ± 0.304

1.064 ± 0.102

3.417 ± 0.228
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski