Amino acid dipepetide frequency for Rhodosalinus sp. E84

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.285AlaAla: 22.285 ± 0.245
1.118AlaCys: 1.118 ± 0.032
7.075AlaAsp: 7.075 ± 0.093
11.355AlaGlu: 11.355 ± 0.149
4.471AlaPhe: 4.471 ± 0.06
12.875AlaGly: 12.875 ± 0.15
2.732AlaHis: 2.732 ± 0.052
5.259AlaIle: 5.259 ± 0.07
2.676AlaLys: 2.676 ± 0.05
16.456AlaLeu: 16.456 ± 0.171
3.968AlaMet: 3.968 ± 0.048
2.201AlaAsn: 2.201 ± 0.046
7.692AlaPro: 7.692 ± 0.11
4.596AlaGln: 4.596 ± 0.067
12.723AlaArg: 12.723 ± 0.163
4.968AlaSer: 4.968 ± 0.071
6.031AlaThr: 6.031 ± 0.084
9.431AlaVal: 9.431 ± 0.098
1.724AlaTrp: 1.724 ± 0.04
2.461AlaTyr: 2.461 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
1.144CysAla: 1.144 ± 0.032
0.086CysCys: 0.086 ± 0.009
0.521CysAsp: 0.521 ± 0.021
0.441CysGlu: 0.441 ± 0.022
0.286CysPhe: 0.286 ± 0.017
0.941CysGly: 0.941 ± 0.033
0.245CysHis: 0.245 ± 0.018
0.337CysIle: 0.337 ± 0.018
0.113CysLys: 0.113 ± 0.01
0.787CysLeu: 0.787 ± 0.025
0.122CysMet: 0.122 ± 0.009
0.172CysAsn: 0.172 ± 0.012
0.508CysPro: 0.508 ± 0.019
0.163CysGln: 0.163 ± 0.012
0.631CysArg: 0.631 ± 0.026
0.338CysSer: 0.338 ± 0.016
0.402CysThr: 0.402 ± 0.021
0.563CysVal: 0.563 ± 0.024
0.106CysTrp: 0.106 ± 0.01
0.177CysTyr: 0.177 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
7.715AspAla: 7.715 ± 0.107
0.536AspCys: 0.536 ± 0.023
3.068AspAsp: 3.068 ± 0.072
3.612AspGlu: 3.612 ± 0.06
2.152AspPhe: 2.152 ± 0.052
5.311AspGly: 5.311 ± 0.078
1.241AspHis: 1.241 ± 0.032
2.521AspIle: 2.521 ± 0.058
1.109AspLys: 1.109 ± 0.032
5.993AspLeu: 5.993 ± 0.077
1.478AspMet: 1.478 ± 0.034
0.914AspAsn: 0.914 ± 0.029
3.973AspPro: 3.973 ± 0.061
1.34AspGln: 1.34 ± 0.037
5.074AspArg: 5.074 ± 0.073
1.902AspSer: 1.902 ± 0.047
2.571AspThr: 2.571 ± 0.059
3.748AspVal: 3.748 ± 0.06
1.327AspTrp: 1.327 ± 0.034
1.531AspTyr: 1.531 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
11.001GluAla: 11.001 ± 0.142
0.305GluCys: 0.305 ± 0.018
3.681GluAsp: 3.681 ± 0.066
4.675GluGlu: 4.675 ± 0.095
1.651GluPhe: 1.651 ± 0.041
6.369GluGly: 6.369 ± 0.085
1.23GluHis: 1.23 ± 0.03
3.827GluIle: 3.827 ± 0.058
1.743GluLys: 1.743 ± 0.048
5.666GluLeu: 5.666 ± 0.087
1.995GluMet: 1.995 ± 0.052
1.398GluAsn: 1.398 ± 0.037
3.065GluPro: 3.065 ± 0.063
1.815GluGln: 1.815 ± 0.038
5.911GluArg: 5.911 ± 0.088
2.518GluSer: 2.518 ± 0.05
4.235GluThr: 4.235 ± 0.067
5.126GluVal: 5.126 ± 0.078
0.729GluTrp: 0.729 ± 0.025
0.965GluTyr: 0.965 ± 0.033
0.0GluXaa: 0.0 ± 0.0
Phe
4.71PheAla: 4.71 ± 0.068
0.35PheCys: 0.35 ± 0.017
2.613PheAsp: 2.613 ± 0.05
2.309PheGlu: 2.309 ± 0.048
1.327PhePhe: 1.327 ± 0.041
3.522PheGly: 3.522 ± 0.066
0.717PheHis: 0.717 ± 0.023
1.211PheIle: 1.211 ± 0.037
0.537PheLys: 0.537 ± 0.023
3.364PheLeu: 3.364 ± 0.061
0.721PheMet: 0.721 ± 0.024
0.792PheAsn: 0.792 ± 0.026
1.558PhePro: 1.558 ± 0.036
0.84PheGln: 0.84 ± 0.028
2.45PheArg: 2.45 ± 0.055
1.703PheSer: 1.703 ± 0.038
1.896PheThr: 1.896 ± 0.04
2.611PheVal: 2.611 ± 0.054
0.525PheTrp: 0.525 ± 0.022
0.823PheTyr: 0.823 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
11.98GlyAla: 11.98 ± 0.122
0.864GlyCys: 0.864 ± 0.028
4.652GlyAsp: 4.652 ± 0.062
5.622GlyGlu: 5.622 ± 0.077
3.567GlyPhe: 3.567 ± 0.056
8.155GlyGly: 8.155 ± 0.112
1.986GlyHis: 1.986 ± 0.042
4.002GlyIle: 4.002 ± 0.057
2.245GlyLys: 2.245 ± 0.058
9.749GlyLeu: 9.749 ± 0.107
2.688GlyMet: 2.688 ± 0.054
1.588GlyAsn: 1.588 ± 0.043
4.429GlyPro: 4.429 ± 0.069
2.761GlyGln: 2.761 ± 0.053
7.589GlyArg: 7.589 ± 0.096
3.656GlySer: 3.656 ± 0.064
4.648GlyThr: 4.648 ± 0.065
6.697GlyVal: 6.697 ± 0.087
1.693GlyTrp: 1.693 ± 0.042
2.218GlyTyr: 2.218 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
2.612HisAla: 2.612 ± 0.045
0.215HisCys: 0.215 ± 0.014
1.239HisAsp: 1.239 ± 0.033
1.083HisGlu: 1.083 ± 0.028
0.823HisPhe: 0.823 ± 0.029
2.073HisGly: 2.073 ± 0.043
0.524HisHis: 0.524 ± 0.024
0.749HisIle: 0.749 ± 0.028
0.344HisLys: 0.344 ± 0.019
2.105HisLeu: 2.105 ± 0.041
0.491HisMet: 0.491 ± 0.02
0.36HisAsn: 0.36 ± 0.018
1.5HisPro: 1.5 ± 0.038
0.478HisGln: 0.478 ± 0.019
1.556HisArg: 1.556 ± 0.036
0.737HisSer: 0.737 ± 0.024
0.743HisThr: 0.743 ± 0.027
1.653HisVal: 1.653 ± 0.042
0.355HisTrp: 0.355 ± 0.018
0.582HisTyr: 0.582 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
6.692IleAla: 6.692 ± 0.08
0.48IleCys: 0.48 ± 0.019
3.046IleAsp: 3.046 ± 0.055
3.289IleGlu: 3.289 ± 0.058
1.449IlePhe: 1.449 ± 0.036
4.194IleGly: 4.194 ± 0.064
0.767IleHis: 0.767 ± 0.025
1.285IleIle: 1.285 ± 0.038
0.819IleLys: 0.819 ± 0.029
4.281IleLeu: 4.281 ± 0.069
0.804IleMet: 0.804 ± 0.03
0.89IleAsn: 0.89 ± 0.031
2.1IlePro: 2.1 ± 0.047
0.851IleGln: 0.851 ± 0.027
3.262IleArg: 3.262 ± 0.054
2.075IleSer: 2.075 ± 0.045
2.111IleThr: 2.111 ± 0.048
3.515IleVal: 3.515 ± 0.063
0.605IleTrp: 0.605 ± 0.026
0.959IleTyr: 0.959 ± 0.028
0.0IleXaa: 0.0 ± 0.0
Lys
2.872LysAla: 2.872 ± 0.054
0.139LysCys: 0.139 ± 0.011
1.095LysAsp: 1.095 ± 0.039
1.16LysGlu: 1.16 ± 0.041
0.549LysPhe: 0.549 ± 0.024
1.921LysGly: 1.921 ± 0.048
0.422LysHis: 0.422 ± 0.019
1.021LysIle: 1.021 ± 0.03
0.717LysLys: 0.717 ± 0.026
2.304LysLeu: 2.304 ± 0.047
0.544LysMet: 0.544 ± 0.022
0.469LysAsn: 0.469 ± 0.021
1.357LysPro: 1.357 ± 0.041
0.549LysGln: 0.549 ± 0.024
1.778LysArg: 1.778 ± 0.049
1.164LysSer: 1.164 ± 0.038
1.25LysThr: 1.25 ± 0.037
1.612LysVal: 1.612 ± 0.044
0.277LysTrp: 0.277 ± 0.018
0.472LysTyr: 0.472 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
15.836LeuAla: 15.836 ± 0.152
0.887LeuCys: 0.887 ± 0.028
6.323LeuAsp: 6.323 ± 0.079
6.366LeuGlu: 6.366 ± 0.083
3.441LeuPhe: 3.441 ± 0.072
9.017LeuGly: 9.017 ± 0.096
1.942LeuHis: 1.942 ± 0.038
4.605LeuIle: 4.605 ± 0.074
2.293LeuLys: 2.293 ± 0.054
9.275LeuLeu: 9.275 ± 0.129
2.52LeuMet: 2.52 ± 0.045
1.961LeuAsn: 1.961 ± 0.042
5.973LeuPro: 5.973 ± 0.073
2.301LeuGln: 2.301 ± 0.046
8.292LeuArg: 8.292 ± 0.095
5.876LeuSer: 5.876 ± 0.074
5.48LeuThr: 5.48 ± 0.064
7.2LeuVal: 7.2 ± 0.094
1.523LeuTrp: 1.523 ± 0.041
2.029LeuTyr: 2.029 ± 0.042
0.0LeuXaa: 0.0 ± 0.0
Met
3.534MetAla: 3.534 ± 0.058
0.145MetCys: 0.145 ± 0.01
1.222MetAsp: 1.222 ± 0.033
1.474MetGlu: 1.474 ± 0.035
0.663MetPhe: 0.663 ± 0.026
2.266MetGly: 2.266 ± 0.048
0.461MetHis: 0.461 ± 0.023
1.213MetIle: 1.213 ± 0.039
0.773MetLys: 0.773 ± 0.027
2.509MetLeu: 2.509 ± 0.048
0.627MetMet: 0.627 ± 0.026
0.654MetAsn: 0.654 ± 0.022
1.587MetPro: 1.587 ± 0.035
0.912MetGln: 0.912 ± 0.026
2.15MetArg: 2.15 ± 0.042
1.449MetSer: 1.449 ± 0.036
1.893MetThr: 1.893 ± 0.039
1.641MetVal: 1.641 ± 0.039
0.235MetTrp: 0.235 ± 0.017
0.288MetTyr: 0.288 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
2.577AsnAla: 2.577 ± 0.045
0.197AsnCys: 0.197 ± 0.013
1.039AsnAsp: 1.039 ± 0.032
0.922AsnGlu: 0.922 ± 0.03
0.712AsnPhe: 0.712 ± 0.027
1.693AsnGly: 1.693 ± 0.044
0.376AsnHis: 0.376 ± 0.019
0.902AsnIle: 0.902 ± 0.031
0.37AsnLys: 0.37 ± 0.018
2.013AsnLeu: 2.013 ± 0.043
0.529AsnMet: 0.529 ± 0.024
0.437AsnAsn: 0.437 ± 0.02
1.515AsnPro: 1.515 ± 0.035
0.472AsnGln: 0.472 ± 0.019
1.471AsnArg: 1.471 ± 0.036
0.699AsnSer: 0.699 ± 0.025
0.967AsnThr: 0.967 ± 0.03
1.491AsnVal: 1.491 ± 0.041
0.314AsnTrp: 0.314 ± 0.017
0.51AsnTyr: 0.51 ± 0.024
0.0AsnXaa: 0.0 ± 0.0
Pro
7.376ProAla: 7.376 ± 0.094
0.336ProCys: 0.336 ± 0.018
4.116ProAsp: 4.116 ± 0.072
5.588ProGlu: 5.588 ± 0.076
1.989ProPhe: 1.989 ± 0.044
5.46ProGly: 5.46 ± 0.074
1.127ProHis: 1.127 ± 0.032
1.854ProIle: 1.854 ± 0.042
1.163ProLys: 1.163 ± 0.036
5.149ProLeu: 5.149 ± 0.079
1.256ProMet: 1.256 ± 0.035
0.966ProAsn: 0.966 ± 0.027
3.123ProPro: 3.123 ± 0.065
1.623ProGln: 1.623 ± 0.039
3.839ProArg: 3.839 ± 0.063
2.285ProSer: 2.285 ± 0.04
2.054ProThr: 2.054 ± 0.037
4.671ProVal: 4.671 ± 0.066
0.832ProTrp: 0.832 ± 0.028
1.141ProTyr: 1.141 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
4.084GlnAla: 4.084 ± 0.062
0.148GlnCys: 0.148 ± 0.012
1.474GlnAsp: 1.474 ± 0.033
1.675GlnGlu: 1.675 ± 0.047
0.858GlnPhe: 0.858 ± 0.026
2.572GlnGly: 2.572 ± 0.052
0.463GlnHis: 0.463 ± 0.021
1.447GlnIle: 1.447 ± 0.039
0.73GlnLys: 0.73 ± 0.027
2.509GlnLeu: 2.509 ± 0.051
0.811GlnMet: 0.811 ± 0.029
0.589GlnAsn: 0.589 ± 0.024
1.489GlnPro: 1.489 ± 0.044
0.863GlnGln: 0.863 ± 0.035
2.057GlnArg: 2.057 ± 0.043
1.314GlnSer: 1.314 ± 0.032
1.449GlnThr: 1.449 ± 0.035
2.121GlnVal: 2.121 ± 0.04
0.314GlnTrp: 0.314 ± 0.018
0.447GlnTyr: 0.447 ± 0.021
0.0GlnXaa: 0.0 ± 0.0
Arg
11.694ArgAla: 11.694 ± 0.133
0.54ArgCys: 0.54 ± 0.021
4.714ArgAsp: 4.714 ± 0.064
5.211ArgGlu: 5.211 ± 0.081
2.972ArgPhe: 2.972 ± 0.052
5.787ArgGly: 5.787 ± 0.075
1.836ArgHis: 1.836 ± 0.042
4.155ArgIle: 4.155 ± 0.064
1.796ArgLys: 1.796 ± 0.05
9.53ArgLeu: 9.53 ± 0.129
2.212ArgMet: 2.212 ± 0.04
1.524ArgAsn: 1.524 ± 0.036
4.557ArgPro: 4.557 ± 0.066
2.365ArgGln: 2.365 ± 0.054
7.43ArgArg: 7.43 ± 0.108
3.082ArgSer: 3.082 ± 0.05
3.351ArgThr: 3.351 ± 0.058
5.63ArgVal: 5.63 ± 0.064
1.206ArgTrp: 1.206 ± 0.034
1.663ArgTyr: 1.663 ± 0.048
0.0ArgXaa: 0.0 ± 0.0
Ser
5.425SerAla: 5.425 ± 0.065
0.344SerCys: 0.344 ± 0.019
2.628SerAsp: 2.628 ± 0.047
2.724SerGlu: 2.724 ± 0.048
1.876SerPhe: 1.876 ± 0.042
4.766SerGly: 4.766 ± 0.068
0.884SerHis: 0.884 ± 0.031
1.8SerIle: 1.8 ± 0.042
0.896SerLys: 0.896 ± 0.03
4.27SerLeu: 4.27 ± 0.06
1.076SerMet: 1.076 ± 0.031
0.884SerAsn: 0.884 ± 0.027
2.401SerPro: 2.401 ± 0.041
1.18SerGln: 1.18 ± 0.031
3.117SerArg: 3.117 ± 0.054
1.843SerSer: 1.843 ± 0.05
1.974SerThr: 1.974 ± 0.044
3.265SerVal: 3.265 ± 0.058
0.593SerTrp: 0.593 ± 0.024
1.046SerTyr: 1.046 ± 0.028
0.0SerXaa: 0.0 ± 0.0
Thr
6.108ThrAla: 6.108 ± 0.067
0.404ThrCys: 0.404 ± 0.019
2.769ThrAsp: 2.769 ± 0.049
3.192ThrGlu: 3.192 ± 0.053
1.692ThrPhe: 1.692 ± 0.04
5.385ThrGly: 5.385 ± 0.078
1.037ThrHis: 1.037 ± 0.031
2.139ThrIle: 2.139 ± 0.048
0.935ThrLys: 0.935 ± 0.031
5.729ThrLeu: 5.729 ± 0.06
1.054ThrMet: 1.054 ± 0.028
0.965ThrAsn: 0.965 ± 0.03
3.349ThrPro: 3.349 ± 0.047
1.295ThrGln: 1.295 ± 0.034
3.746ThrArg: 3.746 ± 0.06
2.031ThrSer: 2.031 ± 0.046
2.342ThrThr: 2.342 ± 0.048
4.103ThrVal: 4.103 ± 0.06
0.692ThrTrp: 0.692 ± 0.025
1.043ThrTyr: 1.043 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
10.15ValAla: 10.15 ± 0.106
0.632ValCys: 0.632 ± 0.022
3.669ValAsp: 3.669 ± 0.061
5.137ValGlu: 5.137 ± 0.064
2.767ValPhe: 2.767 ± 0.053
5.282ValGly: 5.282 ± 0.079
1.352ValHis: 1.352 ± 0.037
3.693ValIle: 3.693 ± 0.068
1.641ValLys: 1.641 ± 0.043
7.557ValLeu: 7.557 ± 0.088
1.956ValMet: 1.956 ± 0.044
1.649ValAsn: 1.649 ± 0.044
3.946ValPro: 3.946 ± 0.062
1.921ValGln: 1.921 ± 0.044
5.067ValArg: 5.067 ± 0.074
3.673ValSer: 3.673 ± 0.064
4.854ValThr: 4.854 ± 0.068
5.71ValVal: 5.71 ± 0.086
1.1ValTrp: 1.1 ± 0.031
1.391ValTyr: 1.391 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.609TrpAla: 1.609 ± 0.038
0.148TrpCys: 0.148 ± 0.011
0.747TrpAsp: 0.747 ± 0.026
0.817TrpGlu: 0.817 ± 0.026
0.575TrpPhe: 0.575 ± 0.023
1.109TrpGly: 1.109 ± 0.031
0.382TrpHis: 0.382 ± 0.02
0.714TrpIle: 0.714 ± 0.027
0.369TrpLys: 0.369 ± 0.018
1.76TrpLeu: 1.76 ± 0.041
0.405TrpMet: 0.405 ± 0.019
0.362TrpAsn: 0.362 ± 0.019
0.795TrpPro: 0.795 ± 0.028
0.576TrpGln: 0.576 ± 0.022
1.43TrpArg: 1.43 ± 0.034
0.761TrpSer: 0.761 ± 0.025
0.777TrpThr: 0.777 ± 0.027
0.896TrpVal: 0.896 ± 0.033
0.26TrpTrp: 0.26 ± 0.017
0.263TrpTyr: 0.263 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.692TyrAla: 2.692 ± 0.051
0.208TyrCys: 0.208 ± 0.014
1.423TyrAsp: 1.423 ± 0.035
1.254TyrGlu: 1.254 ± 0.04
0.76TyrPhe: 0.76 ± 0.026
2.089TyrGly: 2.089 ± 0.044
0.498TyrHis: 0.498 ± 0.023
0.74TyrIle: 0.74 ± 0.024
0.407TyrLys: 0.407 ± 0.021
2.137TyrLeu: 2.137 ± 0.05
0.437TyrMet: 0.437 ± 0.022
0.434TyrAsn: 0.434 ± 0.02
1.031TyrPro: 1.031 ± 0.028
0.512TyrGln: 0.512 ± 0.022
1.684TyrArg: 1.684 ± 0.038
0.918TyrSer: 0.918 ± 0.028
0.98TyrThr: 0.98 ± 0.031
1.461TyrVal: 1.461 ± 0.038
0.345TyrTrp: 0.345 ± 0.017
0.501TyrTyr: 0.501 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3680 proteins (1155616 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski