Pectobacterium phage Arno160

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Melnykvirinae; Wanjuvirus; Pectobacterium virus Arno160

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8F216|A0A3G8F216_9CAUD Scaffolding protein OS=Pectobacterium phage Arno160 OX=2488835 GN=Arno160_gp34 PE=4 SV=1
MM1 pKa = 7.47AAIADD6 pKa = 4.07EE7 pKa = 4.56TEE9 pKa = 3.96LVYY12 pKa = 10.78KK13 pKa = 10.81VGDD16 pKa = 3.33IVKK19 pKa = 9.75IVMYY23 pKa = 10.43NYY25 pKa = 10.78GLADD29 pKa = 3.75TLRR32 pKa = 11.84GYY34 pKa = 11.33YY35 pKa = 8.64STEE38 pKa = 3.6EE39 pKa = 4.4DD40 pKa = 3.36NSVSHH45 pKa = 6.58PVGSIHH51 pKa = 7.25PVTATASGPFGIEE64 pKa = 3.79LGSLCFDD71 pKa = 3.3VSEE74 pKa = 4.6LEE76 pKa = 4.06IVKK79 pKa = 10.36YY80 pKa = 10.55AA81 pKa = 3.61

Molecular weight: 8.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8F4J0|A0A3G8F4J0_9CAUD Uncharacterized protein OS=Pectobacterium phage Arno160 OX=2488835 GN=Arno160_gp18 PE=4 SV=1
MM1 pKa = 6.6NQVVFVCDD9 pKa = 3.57GTTKK13 pKa = 10.19TRR15 pKa = 11.84DD16 pKa = 3.53AQLTAILSAVSVAKK30 pKa = 10.01FEE32 pKa = 4.25RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84NVSKK39 pKa = 10.37EE40 pKa = 3.75AHH42 pKa = 5.82AKK44 pKa = 9.88ALEE47 pKa = 4.08GLRR50 pKa = 11.84RR51 pKa = 11.84EE52 pKa = 4.43YY53 pKa = 10.68SRR55 pKa = 11.84KK56 pKa = 9.04AAAVGMSLRR65 pKa = 11.84GYY67 pKa = 8.79CARR70 pKa = 11.84FNVRR74 pKa = 11.84LPCC77 pKa = 4.93

Molecular weight: 8.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

12927

46

1273

269.3

29.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.971 ± 0.494

1.029 ± 0.143

5.833 ± 0.233

5.987 ± 0.299

3.659 ± 0.227

7.736 ± 0.233

1.98 ± 0.18

4.433 ± 0.228

5.284 ± 0.329

8.563 ± 0.283

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.994 ± 0.185

4.077 ± 0.285

3.845 ± 0.236

4.332 ± 0.288

5.717 ± 0.264

6.537 ± 0.333

6.073 ± 0.413

7.055 ± 0.295

1.323 ± 0.116

3.574 ± 0.203
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski